Chemicals/Reagents

<h2 data-section-id="v1drft" data-start="0" data-end="43">What are cloning chemicals and reagents?</h2>
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Cloning chemicals and reagents cover the enzymes, buffers, and molecular biology-grade chemicals used in DNA fragment preparation, modification, and purification within a cloning workflow, including restriction endonucleases, T4 DNA ligase and other DNA-modifying enzymes (phosphatases, polymerases), reaction buffers, and plasmid extraction reagents. Related entities include restriction enzyme digestion, DNA dephosphorylation/phosphorylation, plasmid DNA purification (column-based and magnetic bead-based), and enzyme unit definitions.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Targeted XJa and XJb strains for faster recovery of proteins.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mix &amp; Go! solutions for optimized and dependable bacterial transformation.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Culture Media &amp; Supplements used for yeast research, and include YPD Plus and mating pheromones.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Extraction and Lysis Reagents include reliable genomic preparation and yeast protein kits.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Chromogenic Substrates and Enzymatic Inducers with highly pure X-GAL and IPTG for cloning and blue-white screening.</li>
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<h2 data-section-id="1daajgc" data-start="866" data-end="913">How to choose cloning chemicals and reagents</h2>
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Restriction enzyme selection and buffer compatibility
Restriction enzymes require specific reaction buffers, and many modern enzyme lines use a single universal buffer compatible across multiple enzymes to simplify double-digest reactions; confirm buffer compatibility when combining enzymes from different suppliers or using older single-buffer-per-enzyme formulations.
Enzyme unit definitions and reaction scale
Restriction enzyme and ligase activity is defined in units per microliter, with one unit typically defined as the amount needed to digest or ligate a defined quantity of standard substrate DNA under specified conditions in a set time; scale enzyme quantity to the amount of DNA being processed rather than assuming a fixed volume works for all reaction sizes.
Plasmid purification grade
Plasmid DNA extraction reagents range from molecular biology grade, suitable for general cloning and sequencing, to transfection-grade or endotoxin-free formulations required for applications such as lentiviral packaging or mammalian cell transfection, where endotoxin contamination can reduce transfection efficiency or cause cell stress.
Dephosphorylation for preventing vector self-ligation
Alkaline phosphatase treatment of a linearized vector before ligation removes 5' phosphate groups, preventing vector self-ligation and improving the ratio of recombinant clones; this step is particularly useful when the vector and insert share compatible sticky ends that could otherwise religate without insert incorporation.
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<h2 data-section-id="1fljvas" data-start="3459" data-end="3484">Specifications context</h2>
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Restriction enzymes and ligases are supplied at defined unit concentrations (commonly 10&ndash;20 units per microliter for restriction enzymes), with reaction buffers formulated for specific pH and salt conditions optimized for enzyme activity. Plasmid purification kits are differentiated by yield (micrograms of DNA recovered), purity (A260/A280 ratio), and endotoxin level (endotoxin units per microgram), with endotoxin-free kits specifying levels below a defined threshold suitable for transfection applications.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and transformation kits and competent cells, used downstream of enzyme-based fragment preparation. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm enzyme buffer compatibility and plasmid purification grade for a specific downstream application before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote for high-purity chemicals and reagents.

MSDS

Protocol

Alpha-Factor Mating Pheromone (240 µl)

Convenient aqueous solution for arresting yeast in G1 of the cell cycle.

Amino Acid Sequence

Concentration

Molecular Weight

Product Storage

Purity

Usage

Manufacturer

Shipping Condition

Storage Condition

DataSheet

IPTG (Isopropyl-β-D-thiogalactopyranoside)

I1001-25 is IPTG (Isopropyl-β-D-thiogalactopyranoside), CAS 367-93-1, MW 238.3 g/mol — supplied as a premade 0.5 M sterile solution (25 mL). The standard non-metabolizable lac operon inducer for recombinant protein expression in E. coli. Typical working concentration: 0.1–1 mM. Store at −20°C.

Isopropyl-β-D-thiogalactopyranoside (IPTG) (5 x 5 ml)

Isopropyl-β-D-thiogalactopyranoside (IPTG) (5 ml)

Mix & Go! 2X Stock Competent Buffer

Mix & Go! 2X Stock Competent Buffer is designed to be used with our Mix & Go! E. coli Transformation Kit and Buffer Set, which is used to prepare highly competent E. coli cells for simple 20 second DNA transformations.

Mix & Go! 2X Stock Competent Buffer (10 ml)

Mix & Go! 2X Stock Competent Buffer (30 ml)

Mix & Go! 2X Stock Wash Buffer

Mix & Go! 2X Wash Buffer is designed to be used with our Mix & Go! E. coli Transformation Kit and Buffer Set, which is used to prepare highly competent E. coli cells for simple 20 second DNA transformations.

Mix & Go! 2X Stock Wash Buffer (10 ml)

Mix & Go! 2X Stock Wash Buffer (30 ml)

Mix & Go! Dilution Buffer

Mix & Go! Dilution Buffer is designed to be used with our Mix & Go! E. coli Transformation Kit and Buffer Set, which is used to prepare highly competent E. coli cells for simple 20 second DNA transformations.

Mix & Go! Dilution Buffer (20 ml)

Mix & Go! Dilution Buffer (60 ml)

Rattler Plating Beads

4.5 mm glass plating beads for spreading inocula

Material

Packaging

Rattler™ Plating Beads (230 g Bottle)

Rattler™ Plating Beads (5 x 230 g Bottles)

Rattler™ Plating Beads, Bulk (25 kg Bag)

X-GAL (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside)

Ready-to-use sterile solution for detecting Œ≤-galactosidase activity.

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) (5 x 5 ml)

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) (5 ml)

XJa (DE3) Autolysis Glycerol Stock (1 Tube)

XJa (DE3) Autolysis Glycerol Stock T5031 by Zymo Research — 1 mL glycerol stock with 500× L-arabinose — provides 80–90% cell lysis after a single freeze-thaw cycle (~10 min post-harvest). The DE3 lysogen supports T7 promoter–based recombinant protein expression; growth requires 1 mM Mg²⁺ supplementation. Storage at –70°C to –80°C. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Autolysis

Cell Growth

DNA Extraction

DNA Stability

Genotype

Processing Time

Protein Expression

XJa Autolysis Glycerol Stock (1 Tube)

Simple, fast, and controlled autolysis of E. coli for protein expression and purification.

XJb (DE3) Autolysis Glycerol Stock (1 Tube)

XJb Autolysis Glycerol Stock (1 Tube)

Y-Lysis Buffer

Y-Lysis Buffer is designed to be used with our Yeast Protein Kit, which provides a rapid method for efficient lysis of a broad spectrum of fungus species using Zymolyase for downstream protein and DNA analysis.