Sealing Films

PCR & qPCR Film

Sealing Mats - Accessories

DataSheet

96 Well Square well Silicone Sealing Mat, can be punctured

384 Well Square well Silicone Sealing Mat

Square Well Silicone Sealing Mats

96 Well Square well Silicone Sealing Mat, Non-Sterile

506003 by NEST Scientific is a reusable 96-well square-well silicone sealing mat for deep well plates, non-sterile, 50/pack. Silicone construction tolerates -80°C to +220°C, resists solvents and bases, and leaves no adhesive residue. Designed for compound storage, HTS plates, and sample incubation requiring repeated plate access.

Manufacturer

96 Well Square well Silicone Sealing Mat, non-sterile, 10/pk, 50/cs

96 Well Square well Silicone Sealing Mat, can been punctured, non-sterile, 10/pk, 50/cs

24 Well Square well Silicone Sealing Mat, non-sterile, 10/pk, 50/cs

24 Well Square well Silicone Sealing Mat, Sterile, 10/pk, 50/cs

48 Well Square well Silicone Sealing Mat, non-sterile, 10/pk, 50/cs

48 Well Square well Silicone Sealing Mat, Sterile, 10/pk, 50/cs

384 Well Square well Silicone Sealing Mat, non-sterile, 10/pk, 50/cs

384 Well Square well Silicone Sealing Mat, Sterile, 10/pk, 50/cs

96 Well Square well Silicone Sealing Mat, Sterile, 10/pk, 50/cs

96 Well Square well Silicone Sealing Mat, can been punctured, Sterile, 10/pk, 50/cs

Adhesive aluminum Sheet for deep well plates and PCR plates, 130mm x 80mm, 100/pk

520003 by NEST Scientific is a 130 × 80 mm adhesive aluminum sealing sheet for all SBS-format deep well and PCR plates. Acrylamide adhesive, 0.18 mm thick, pierceable, light-blocking, and VOC-resistant. Lot-tested free of pyrogens, DNase/RNase, and BSE/TSE. 100/pk.

Adhesive sealing film for deep well plates, 100/pk

520001 by NEST is a transparent peel-and-stick adhesive sealing film for 96-well deep well plates, supplied 100 sheets per pack. The film seals all wells simultaneously to prevent evaporation and contamination during storage, incubation, and centrifugation, while remaining transparent for visual well inspection without breaking the seal.

Aluminum Sheet for deep well plates and PCR plates, 100/pk

520002 by NEST Scientific is a pressure-sensitive adhesive aluminum sealing sheet for 96-well PCR plates and deep well plates, 130 mm × 80 mm, 100 sheets/pack. No heat sealer required. Impermeable barrier against evaporation, cross-contamination, and light — suitable for compound storage, HTS sample plates, and PCR plate archiving.

Datsheet

NEST PCR Plates

PCR Sealing Film Specifications

PCR Sealing Film

410001 by NEST Scientific is an adhesive PCR sealing film (146 × 81 mm) for standard 96-well PCR and ELISA plates, providing an evaporation-resistant seal through high-temperature PCR cycles without a heat sealer instrument, supplied 100/pk and 500/cs.

PCR sealing film, Adhesive, 146 x 81 mm, 100/pk, 500/cs

PCR sealing film, heat sealing, 141 x 78 mm, 100/pk, 500/cs

PCR film scraper

411001 by NEST Scientific is a reusable polypropylene PCR film scraper/applicator (70 × 78 mm) that presses adhesive PCR sealing films uniformly onto 96-well PCR and ELISA plates, ensuring complete adhesion across all well rims and preventing evaporation or contamination during thermal cycling. Sold individually.

96 Well Round well Silicone Sealing Mat, can be punctured, <=1.0 ml

96 Well Round well Silicone Sealing Mat, 2.0 ml

96 Well Round well Silicone Sealing Mat, pre-slit, 2.0 ml

Round well Silicone Sealing Mat

Nest Scientific 506005 — 96-well round-well pre-slit silicone sealing mat, 7 mm well diameter, fits 0.35–1.35 mL deep well plates, non-sterile, 10 per pack, 50 per case.

96 Well Round well Silicone Sealing Mat, pre-slit, <=1.0 ml, non-sterile, 10/pk, 50/cs

96 Well Round well Silicone Sealing Mat, 2.0 ml, non-sterile, 10/pk, 50/cs

96 Well Round well Silicone Sealing Mat, pre-slit, 2.0 ml, non-sterile, 10/pk, 50/cs

96 Well Round well Silicone Sealing Mat, pre-slit, 1.0 ml, Sterile, 10/pk, 50/cs

96 Well Round well Silicone Sealing Mat, 2.0 ml, Sterile, 10/pk, 50/cs

<h2 dir="ltr">What are PCR and qPCR sealing films?</h2>
 PCR and qPCR sealing films are optically clear microplate seals applied to 96- and 384-well PCR plates to create an airtight barrier through every thermal cycle, preventing evaporation, condensation, and cross-well contamination while still letting fluorescence pass through to the qPCR detector. Most are pressure-sensitive adhesive (PSA) or heat-activated films built on polypropylene or polyester (PET) with low-autofluorescence chemistry. Standard formats fit SBS sheets of approximately 137 &times; 80 mm. Buy from this category whenever the seal sits on a plate that will be thermally cycled or read in real time, not stored or incubated under simple lids. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Optical-grade qPCR sealing films for fluorescence-based detection</li>
<li dir="ltr" role="presentation">Standard adhesive PCR films for routine thermal cycling</li>
<li dir="ltr" role="presentation">96-well microplate sealing films for standard PCR workflows</li>
<li dir="ltr" role="presentation">384-well compatible sealing films for high-throughput assays</li>
<li dir="ltr" role="presentation">High-temperature resistant films for -40&deg;C to 120&deg;C cycling</li>
<li dir="ltr" role="presentation">Low-evaporation sealing films for reaction stability</li>
<li dir="ltr" role="presentation">DNase/RNase-free films for contamination-sensitive assays</li>
</ul>
&nbsp;
<h2 dir="ltr">How to choose PCR or qPCR sealing film</h2>
 Confirm true optical clarity if you read fluorescence Not every "clear" sealing film has enough optical clarity and low enough autofluorescence for real-time PCR; choose a film specifically rated for qPCR with high light transmittance and no autofluorescence so excitation and emission pass cleanly. Standard storage films can suppress the signal. Decide between adhesive (PSA) and heat-seal Pressure-sensitive adhesive films are applied with a roller or paddle at the bench and peel off; heat-seal films bond under a plate sealer at controlled temperature and pressure for the strongest barrier in high-throughput and automated qPCR. PSA suits flexible workflows; heat-seal suits volume. Match the plate, thermal cycler, and skirt Confirm the film fits 96- or 384-well plates from your supplier and seats properly under the heated lid of your thermal cycler &mdash; full-skirt, semi-skirt, and non-skirt plates have different sealing surfaces. Sheet size near 137 &times; 80 mm is the SBS norm. Check chemical and temperature resistance PCR plate sealers and thermal cyclers reach 105 &deg;C routinely, and some films are rated to 150 &deg;C; check that the film and adhesive withstand the cycler protocol and the master mix without softening, lifting at corners, or leaving residue after peeling.
&nbsp;
<h2 dir="ltr">Specifications context</h2>
 Quality qPCR films are defined by light transmittance (typically &ge; 90 %), absence of autofluorescence, evaporation rate (commonly &le; 3 %), thermal tolerance to about 105 &deg;C with high-temperature variants to 150 &deg;C, and a DNase/RNase- and human-DNA-free certification appropriate for amplification. Most are supplied as flat SBS-format sheets around 137 &times; 80 mm, packed 100 per box; some have perforated end tabs for one-handed peeling. As of 2026, they sit in a low-to-mid price tier, with heat-seal films at the higher end.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Connect with the MBP team today</a>&nbsp;to find the perfect optical or standard seal for your amplification workflow. Browse the wider&nbsp;<a href="https://mbpinc.net/consumables/sealing-films">sealing films range </a>for post-PCR archiving and reorder today via <a href="https://mbpinc.net/quickorder">Quick Order</a>.

ThermalSeal RTS Optically Clear, Silicone Adhesive

ThermalSeal RTS™ (TSS-RTQ-100) by MBP is an optically clear, silicone-adhesive plate seal for qPCR, long-term storage, protein crystallography, and HPLC. Direct alternative to Thermofisher 4311971 MicroAmp Optical Adhesive Film. Available in 100-seal (TSS-RTQ-100), 25-film (TSS-RTQ-25), and sterile 50-film (TSS-RTQS-50, &minus;80&deg;C to +105&deg;C) formats. Ships ambient.

Application

Temperature Range

Optical Adhesive Films For PCR, Storage & Crystallization - Replaces 4311971 by Thermofisher 100 Seals/Case

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, 25 Sealing Films/Pack

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, Sterile, qPCR, long-term storage, protein crystallography & HPLC, -80°C to +105°C, 50 Sealing Films/Pack