Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
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Sterile Bottle-Top Filters for secure aseptic transfer
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<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Adaptor for Vaccum Filtration System, 45mm to 38mm, 1/pk, 10/cs

334591 by NEST Scientific is a polypropylene adaptor that converts a 45 mm vacuum filtration port to a 38 mm connection, compatible with NEST disposable vacuum filtration systems in 250 mL, 500 mL, and 1000 mL formats. Supplied 1/pk, 10/cs for lab and bioprocessing filtration setups.

Manufacturer

Datsheet

Vacuum Filtration Systems  (PES Membrane)

Bottle Top Vacuum Filter, PES, Sterile

347012 by NEST is a 50 mL bottle top vacuum filter with a 0.1 micrometer hydrophilic PES membrane (50mm diameter), E-beam sterilized to SAL 10^-6, with low protein binding and high flow rate. PS cup and cap; ABS neck; max 45 degrees C working temperature. Not for chloroform, esters, or strong acids/bases. Supplied 1/sterile bag, 24/case.

50 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

50 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

50 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

Vacuum Filtration Systems (PVDF Membrane)

Bottle Top Vacuum Filter, PVDF, Sterile

347112 by NEST Scientific is a sterile 50 mL bottle top vacuum filter with a 0.10 µm PVDF membrane, E-beam sterilized to SAL 10⁻⁶, manufactured in an ISO 7 cleanroom, certified DNase/RNase-free and non-pyrogenic, for protein solution filtration, buffer sterilization, and media preparation.

50 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

50 mL Bottle Top Vacuum Filter, 0.22μm, PVDF, Sterile,1/pk, 24/cs

50 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuum Filter, 0.22μm, PVDF, Sterile,1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.45μm, PVDF, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

Vacuum Filtration Systems, PES, Sterile

347002 by NEST is a sterile, disposable vacuum filtration system with a PES membrane. Available in five volumes (50–1000 mL) and three pore sizes (0.10 µm, 0.22 µm, 0.45 µm) as selectable variants. Polystyrene USP Grade IV cup with ABS neck, E-beam sterilized to SAL 10⁻⁶. Non-pyrogenic, DNase/RNase-free, animal product-free. 12/case.

50 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

50 mL Vacuum Filtration, 0.22 μm, PES, Sterile,1/pk, 12/cs

50 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

150 mL Vacuum Filtration, 0.22 μm, PES, Sterile,1/pk, 12/cs

150 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

250 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

500 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.10 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.22 μm, PES, Sterile, 1/pk, 12/cs

1000 mL Vacuum Filtration, 0.45 μm, PES, Sterile, 1/pk, 12/cs

Vacuum Filtration Systems, PVDF, Sterile

347102 by NEST Scientific is a sterile disposable vacuum filtration system with a PVDF membrane, E-beam sterilized (SAL 10⁻⁶), DNase/RNase-free, and endotoxin-free. The PVDF membrane offers low protein binding and high flow rates for cell culture media sterilization, buffer filtration, and protein solution processing. PS filter top and PS receiving bottle with ABS neck; separate sterile PE cap included. Multiple volume options available.