Cell Culture Dishes

<h2 dir="ltr"> What are Cell Culture Dishes?</h2>
 Cell culture dishes are sterile, shallow, circular polystyrene containers with a lid for growing adherent or suspension mammalian cells, bacteria, yeast, and microorganisms in a CO₂ incubator or ambient environment. TC-treated (tissue culture&ndash;treated) dishes support adherent mammalian cell monolayer growth; non-treated dishes suit suspension cells, colony counting, and bacteriological applications. Choose the dish diameter based on required cell number and downstream assay volume: 35 mm for small-scale primary culture and microscopy; 60 mm for transfection and mid-scale assays; 100 mm for routine large-scale expansion, protein isolation, and virus production; 150 mm for maximum single-vessel cell yield.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="zaop1j" data-start="0" data-end="134">TC-treated and sterile cell culture dishes in standard, polystyrene (PS), Diamond SureGro, gripping ring, and external grip formats.</li>
<li data-section-id="1tw8fwo" data-start="136" data-end="215">Glass bottom cell culture dishes for microscopy-based cell culture workflows.</li>
<li data-section-id="sr7dgd" data-start="217" data-end="298">Spheroid culture products, including Spheroid Dishes and Spheroid Forming Units.</li>
<li data-section-id="s6nepr" data-start="300" data-end="358">3D Cell Floater Dishes for 3D cell culture applications.</li>
<li data-section-id="4r7o73" data-start="360" data-end="422" data-is-last-node="">Multi-Insert Dishes for multi-insert culture configurations.</li>
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<h2 dir="ltr"> How to Choose Cell Culture Dishes</h2>
 Diameter and Growth Area by Application 35 mm dishes (growth area approximately 8.8 cm&sup2;, working volume 2 mL) are the standard for primary cell culture, colony counting, clonal selection, and high-resolution phase-contrast microscopy from adherent monolayers, where the small diameter matches the field of view of low-magnification objectives. 60 mm dishes (growth area approximately 21 cm&sup2;, working volume 5 mL) suit transfection experiments (calcium phosphate, lipofection, PEI) and mid-scale protein or RNA harvest. 100 mm dishes (growth area approximately 55 cm&sup2;, working volume 10 mL) are the routine format for cell expansion, large-scale transfection (AAV, lentivirus production), and whole-cell protein or nucleic acid extraction. 150 mm dishes (growth area approximately 148 cm&sup2;, working volume 20&ndash;30 mL) provide maximum cell yield from a single vessel for high-yield protein production, ChIP-seq input material, and large-scale virus production. TC-Treated vs. Non-Treated Dishes TC-treated NEST dishes have vacuum plasma&ndash;modified surfaces (contact angle &lt;10&deg;) supporting adherent mammalian cell attachment, equivalent to TC-treated T-flask surfaces. Non-treated dishes (when available) maintain the hydrophobic PS surface for suspension cell maintenance, organoid culture, and bacteriological applications. Confirm surface treatment from the product label before seeding; TC-treated and non-treated dishes are visually identical. Gripping Ring Option for 100 mm Dishes NEST Scientific 100 mm dishes are available with and without an Easy-Grip gripping loop molded into the dish wall. The gripping ring provides a secure handhold through latex or nitrile gloves in a biosafety cabinet, reducing slip-and-drop contamination events during media changes and trypsin addition for large-batch 100 mm dish workflows. For labs handling &gt;20 dishes per day, gripping ring dishes are a practical quality-of-life improvement. Optical Clarity and Microscopy NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene with a flat, distortion-free growth surface, allowing phase-contrast microscopy and standard fluorescence widefield microscopy directly in the dish without cell transfer. Numeric position marks on the dish bottom allow users to re-locate specific fields between imaging sessions. For confocal, TIRF, or super-resolution imaging requiring No. 1.5 coverglass, use glass-bottom dishes or [glass-bottom cell culture plates](mbpinc.net/consumables/cell-culture/cell-culture-plates/glass-bottom-cell-culture-plates) instead. Stackability and Incubator Efficiency NEST Scientific cell culture dishes feature a raised rim geometry and a snap-fit lid that allow stable stacking in a CO₂ incubator. Stacking feet on the lid prevent the lid from sliding and ensure the inner lid face does not contact the dish rim of the dish below &mdash; maintaining sterility of the lid's inner surface during stacking. For high-throughput cell culture labs running &gt;50 dishes simultaneously, dish stackability significantly increases effective incubator shelf utilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per lot (criterion: contact angle &lt;10&deg;). E-beam sterilized to SAL 10⁻⁶; certified DNase/RNase-free and non-pyrogenic per lot. Flat bottom for distortion-free phase-contrast microscopy; numeric position marks on dish floor. NEST Scientific is ISO 9001:2015, ISO 13485, and ISO 11137 certified with FDA registration and CE marking. As of 2026, NEST Scientific dishes in all four sizes are stocked by MBP. 
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Don&rsquo;t compromise on cultural integrity. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for&nbsp;high-performance labware. Request a quote today to streamline your laboratory workflow.

DataSheet

Product Literature

Cell Culture Dish Specifications

Cell Culture Dish, TC Treated, Sterile

706001 by NEST Scientific — 35 mm TC-treated polystyrene cell culture dish, 9 cm&sup2; growth area, 1.8&ndash;2.7 mL working volume, vented lid, sterile, with numeric position indicators. 20/pk, 500/cs.

Manufacturer

35mm Cell Culture Dish, TC, sterile 20/pk, 500/cs

60mm Cell Culture Dish, TC, sterile 20/bag, 500/cs

100mm Cell Culture Dish, TC, sterile 5/bag, 300/cs

100mm Cell Culture Dish, TC, sterile 20/bag, 300/cs

150mm Cell Culture Dish, TC, sterile 5/pk,100/cs

Cell Culture Dish, with Gripping Ring Specifications

Cell Culture Dish, with Gripping Ring, TC Treated, Sterile

The 706201 by NEST Scientific is a 35 mm TC-treated sterile cell culture dish manufactured from USP Grade VI virgin polystyrene in an ISO 7 cleanroom. It delivers 8.5 cm² of growth area, plasma-etched for uniform cell attachment, and features numeric position indicators on the dish bottom for microscopic field relocation. Certified pyrogen-free (USP 85), cytotoxin-free (USP 87/88), DNase/RNase-free, and BSE/TSE-free; sterilized by e-beam to SAL 10⁻⁶. Packed 20 per sleeve, 500 per case.

35mm Cell Culture Dish, with Gripping Ring, TC, Sterile, 20/pk, 500/cs

60mm Cell Culture Dish, with Gripping Ring, TC, Sterile, 20/pk, 500/cs

100mm Cell Culture Dish, with Gripping Ring, TC, Sterile 20/bag, 300/cs

Datsheet

Glass Bottom Culture Dishes Specifications

Glass Bottom Cell Culture Dish

801002 by NEST Scientific is a 35 mm glass-bottom cell culture dish with a 15 mm optical-grade glass imaging window (~0.16–0.19 mm thick, #1.5 cover glass equivalent). TC treated, USP Class VI, E-beam sterilized to SAL 10⁻⁶. Designed for confocal, TIRF, super-resolution, DIC, and phase-contrast microscopy. Non-pyrogenic, DNase/RNase/DNA-free. 10/pk, 200/cs.