BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
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BioFactory media transfer caps
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Sterile tubing and connector sets
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Fluid transfer adapters for cell culture vessels
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BioFactory bottle caps and closures
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Sterile quick-connect systems
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Liquid handling accessories
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Vessel handling and support tools
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Closed-system connectors for aseptic processing
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<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Product Literature

NEST BioFactory Adaptor Caps Specifications

Adaptor Caps for BioFactories, Individually Wrapped, Sterile

NEST Scientific 740201 sterile adaptor caps for BioFactory multi-layer cell culture vessels, individually wrapped. Enables closed-system tubing and hose connections to NEST BioFactory ports for media exchange and harvest in large-scale adherent cell culture. Multiple cap types and materials available.

Manufacturer

Adaptor Cap Wide Mouth to Narrow Mouth for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 3/8" ID (9.5 mm) Hose for BioFactories, PP, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 1/4" (5.8 mm) ID Hose for BioFactories, PP, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 3/8" ID (9.5 mm) Hose for BioFactories, HDPE, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Connector to 3/8 Inches(9.5 mm) Hose for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

NEST Scientific 741001 is a sterile individually wrapped adaptor connector for BioFactory multi-layer cell culture vessels, sized for 3/8-inch (9.5 mm) inner-diameter hose lines. Supplies the hose-to-BioFactory port connection in closed-system media transfer and harvest assemblies. 1/pk, 10/cs.

BioFactory Accessory Packages Components

BioFactory Accessory Package

ZME000B by NEST Scientific — BioFactory Accessory Package. Sterile kit includes: hose clamp, 0.22 &mu;m vent filter (62mm), silicone tube (80cm, 1/4&quot; ID &times; 3/8&quot; OD), male CPC connector with sealing cap, and 2 silicone rings. 1/pk, 4/cs. BioFactory vessel sold separately.

BioFactory Accessory Lite Package, Hose Clamp*1+50 mm Vent Filter*1+15 cm SPT-50 Hose*1+Silicone Ring*2, Sterile, 1/pk, 2/cs

BioFactory Accessory Basic Package,Hose Clamp*1+50 mm Vent Filter+15 cm SPT-50 Hose*1+Adaptor Connector*1+Silicone Ring*2, Sterile, 1/pk, 2/cs (Old No.: 745021)

BioFactory Accessory Premiμm Package, 2 different Adaptor Caps*1+Hose Clamp*1+Adaptor Connector*1 +50 mm Vent Filter*1+Hose Clamp*1+15 m SPT-50 Hose*1, Individually Wrapped, Sterile, 1/pk, 1/cs

745001 by NEST Scientific is a sterile BioFactory Accessory Complete Package containing 2 adaptor caps, 2 hose clamps, 1 adaptor connector, 1 × 50 mm vent filter, and 15 m SPT-50 hose — individually wrapped, ISO 8 clean room manufactured, USP Class VI materials, ISO 9001/13485 certified. Provides a complete closed-system fluid-transfer setup for NEST BioFactory cell culture vessels. Available through MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

BioFactory Adaptor Caps and Overcaps Specifications

BioFactory Adaptor Caps and Overcaps

740213 by NEST Scientific is a sterile BioFactory adaptor cap assembly combining the 740201 wide-mouth-to-narrow-mouth HDPE adaptor cap with a vented overcap fitted with a 0.22 μm hydrophobic breathable membrane. It enables aseptic ventilation during liquid transfer on NEST BioFactory 10-chamber and 40-chamber cell culture systems. Individually wrapped, ISO 9001 and ISO 13485 certified. Pack size: 1/pk, 10/cs.

Adaptor Cap(740201) Pre Installed with a Vented Overcap for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Solid Overcap for BioFactories, Adaptable for 740201, Individually Wrapped, Sterile, 1/pk, 20/cs

Vented Overcap for BioFactories, Adaptable for 740201, Individually Wrapped, Sterile, 1/pk, 20/cs

BioFactory Holder, Individually Wrapped, 1/pk, 5/cs

751101 by NEST Scientific is a BioFactory holder designed to support and stabilize NEST BioFactory multi-chamber cell culture systems in the incubator, during media exchange, and in transport. Individually wrapped to maintain surface cleanliness before use. Pack size: 1/pk, 5 packs per case.

C-Flex Welding Hose, Inner dia# 3/8 Inches(9.5 mm)，Out dia# 5/8 Inches(15.9 mm), 50 Inches/ 15 meters, Individually Wrapped, Sterile, 1/pk, 1/cs

746001 by NEST Scientific is a sterile C-Flex-type TPE welding hose with 3/8″ ID (9.5 mm), 5/8″ OD (15.9 mm), and 15-meter length, individually wrapped for BioFactory bioprocess tubing connections. Designed for sterile tube welding and closed-system fluid transfer in biopharmaceutical and cell culture production workflows. Supplied 1/pk, 1/cs.

CPC Connector(Inner Dia #3/8 ) for Hose to Hose Connecting, Individually Wrapped, Sterile, 1/pk, 5/cs

747001 by NEST Scientific — straight hose-to-hose CPC connector, 3/8&quot; inner diameter, individually wrapped, sterile. For single-use bioprocess tubing assemblies. 1/pk, 5/cs.

CPC Sealing Plug, Individually Wrapped, Sterile, 1/pk, 1/cs

747011 by NEST Scientific is a sterile, individually wrapped CPC sealing plug for NEST BioFactory cell culture vessels, sized for #3/8 inch ports (9.5 mm ID / 15.9 mm OD). Manufactured in ISO 8 clean rooms, USP Class VI materials, ISO 9001/13485 certified. 10 pcs/case. Available through MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

BioFactory Cap with Silicon Tube (80cm 1/4" ID3/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile

BioFactory Cap with Silicon Tube (80cm 3/8" ID5/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile

BioFactory Cap with PTFE Vent Filter (0.22 μm Φ42mm), without BioFactory, Sterile

BioFactory Cap with PTFE Vent Filter (0.22 μm Φ50mm), without BioFactory

Filter/inlet & outlet tubing for BioFactory

C71254 by NEST Scientific provides filter, inlet, and outlet tubing accessories for the NEST BioFactory&#8482; multi-layer cell culture system, enabling closed aseptic liquid transfer during vaccine, mAb, and large-scale adherent cell culture workflows. The vent cap incorporates a 0.22&#160;µm PTFE hydrophobic filter; the system is E-beam sterilized to SAL&#160;=&#160;10&#8315;&#8310; with no endotoxin and no animal-origin components.

BioFactory Cap with Silicon Tube (80cm 1/4 " ID3/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile, 1/pk, 4/cs

BioFactory Cap with Silicon Tube (80cm 3/8 " ID5/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile, 1/pk, 4/cs

BioFactory Cap with PTFE Vent Filter(0.22 μm Φ42mm), without BioFactory , Sterile, 1/pk, 4/cs

BioFactory Cap with PTFE Vent Filter(0.22 μm Φ50mm), without BioFactory , Sterile, 1/pk, 4/cs

Funnel for Media Transfer, 60 mm Mouth Diameter, 24mm Tube Diameter, Individually Wrapped, Sterile, 1/pk, 10/cs

NEST Scientific 750001: sterile individually wrapped media transfer funnel with 60 mm mouth diameter and 24 mm tube diameter. Single-use; for aseptic liquid transfer into standard laboratory bottles and vessels. 1/pk, 10/cs. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

Hose Clamp for 12-18 mm diameter hoses, each, Sterile, 1/pk, 10/cs

Sterile hose clamp (743001) for 12–18 mm diameter flexible tubing, compatible with BioFactory and bioprocess tubing assemblies. Supplied 1 per sterile pack, 10 per case — for secure, contamination-free hose sealing in closed cell culture systems.