RNA Extraction Kits

Mag Beads

<h2>What are Magnetic Bead Plant RNA Extraction Kits?</h2>
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Magnetic bead plant RNA extraction kits purify total RNA from plant tissue -- leaves, stems, roots, seeds, and other plant organs -- using mechanical disruption in a specialized plant lysis buffer followed by binding of RNA to paramagnetic silica-coated beads, magnetic separation during successive wash steps to remove polyphenols, polysaccharides, and other plant-specific inhibitors, and elution in RNase-free water without centrifugation for the RNA purification phase. The 96-well format processes all samples simultaneously on liquid-handling robots (KingFisher Flex, Tecan Fluent, Hamilton STAR), enabling the scale needed for plant breeding programs, genome-wide association studies (GWAS) linked to expression, and abiotic stress response screens, processing hundreds of individual plant samples per day. Choose magnetic bead plant RNA extraction when processing more than 48 plant samples per session, when automation infrastructure is available, or when high-polysaccharide lysates cause consistent column clogging in spin-column formats.
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<h2 dir="ltr">What you will find:</h2>
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<li data-section-id="bjjl4q" data-start="25" data-end="128">Magbio HighPrep Plant RNA Plus Kit for rapid isolation of total RNA from complex plant tissues.</li>
<li data-section-id="1r07f8r" data-start="129" data-end="191">Magnetic bead&ndash;based purification for efficient RNA recovery.</li>
<li data-section-id="s295jn" data-start="192" data-end="258">Automation-ready workflow for high-throughput sample processing.</li>
<li data-section-id="6vw6z8" data-start="259" data-end="318">Effective extraction from challenging plant sample types.</li>
<li data-section-id="1u9s43l" data-start="319" data-end="399" data-is-last-node="">High-quality total RNA suitable for downstream molecular biology applications.</li>
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<h2>How to Choose a Magnetic Bead Plant RNA Kit</h2>
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Automation Platform and Script Availability
Confirm the kit manufacturer provides validated automation scripts for your instrument before purchase. Thermo Fisher MagMAX Plant RNA Isolation Kit provides KingFisher Flex and Apex scripts; Zymo Direct-zol-96 MagBead supports Tecan and Hamilton in addition to KingFisher. For other platforms, contact MBP's technical team to confirm script availability or the feasibility of in-house protocol adaptation.
CTAB vs. Guanidinium Lysis
Guanidinium-based lysis buffers are compatible with most magnetic bead RNA kits and are the default for standard plant species. For high-polyphenol species (Vitis, Pinus, Citrus) where guanidinium lysis produces brown, inhibitor-rich lysates, CTAB-based upstream lysis can be adapted for magnetic bead purification by adjusting the lysate-to-ethanol ratio before bead addition; confirm protocol compatibility with the specific kit before attempting CTAB lysis with magnetic bead formats.
Tissue Disruption Before Bead Extraction
Magnetic bead kits do not include mechanical disruption; plant tissue must be ground in liquid nitrogen to a fine powder or bead-beaten in lysis buffer before adding the lysate to the bead extraction plate. For 96-sample throughput, a 96-well bead mill system paired with a 96-deep-well plate is the most efficient tissue disruption setup for plant material at scale.
Small RNA Retention
Confirm the kit retains RNAs &gt;=17 nt from plant tissue, including miRNAs, ta-siRNAs, and siRNAs. Silica bead formats generally retain the full RNA size spectrum; check the kit datasheet for the minimum RNA size retained.
Post-Extraction Quality Assessment
For large batch processing, run a representative subset (1 plate per 5 batches) on a Bioanalyzer RNA Nano chip to monitor RIN values over time. Declining RIN values across batches signal RNase contamination of reagents or incomplete tissue disruption that reduces RNA integrity before bead binding.
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<h2>Specifications Context</h2>
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Quality targets for magnetic bead plant RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, RIN &gt;= 7, and yield of 1-20 ug from 50 mg leaf tissue. For large-scale RNA-seq (&gt;48 samples per run), RNA-seq library preparation kits validated for automated input (Zymo-Seq RiboFree Plant) are required downstream. High-throughput plant RNA-seq for quantitative trait loci (eQTL) studies and genomic selection in crop improvement programs at plant research institutes regularly processes 96-well batches of plant RNA, with magnetic bead extraction enabling the scale these programs require.&nbsp;
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<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP Inc. </a>to get optimized mag bead systems for high-throughput workflows.

HighPrep Plant RNA Plus Kit - Protocol

Magbio HighPrep Plant RNA Plus Kit

The HighPrep Plant RNA Plus Kit by MagBio Genomics purifies RNA from 10–50 mg of plant tissue or 10–20 mg of seeds using MAG-R5 magnetic beads. A260/A280 ≥1.8; integrated DNase I digestion (37°C, 15 min); 50–100 µL elution volume. Removes polysaccharides and phenolic compounds. Compatible with leaves, roots, seeds, flowers, and fungal samples. KingFisher™ Flex automation ready.

Features

Main functions

Starting material (Sample matrix)

Sample amount (Input)

Sample Source

Process method

Instrument/Equipment

Purification method

Elution Volume

Purity

Manufacturer

HighPrep Plant RNA Plus Kit (10 Preps)

HighPrep Plant RNA Plus Kit (96 Preps)

HighPrep Plant RNA Plus Kit (384 Preps)

<h2>What are Magnetic Bead Total RNA Extraction Kits?</h2>
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Magnetic bead total RNA extraction kits isolate total RNA by adsorbing nucleic acids onto paramagnetic silica-coated beads in a high-chaotropic-salt lysis buffer, then using a magnetic field to capture and wash beads without centrifugation. Unlike standard spin-column kits, the bead format removes centrifuge dependency, enabling scalable manual processing in 96-well plates or full automation on liquid-handling robots.&nbsp;Choose a magnetic bead format when your lab processes more than 48 samples per day or when a Tecan, Hamilton, or Beckman Biomek platform is available.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Kits for liquid biopsy collection systems and non-invasive swabbing using rapid magnetic bead isolation&nbsp;
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Zymo-Seq RiboFree&trade; Kits to eliminate ribosomal RNA carryover during library synthesis
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Direct-zol-96 MagBead RNA Modules facilitate complete avoidance of manual phase-separation steps while enabling automated transcript extraction directly from TRIzol&reg; lysates&nbsp;
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Total RNA Plus Kit extracts pure, inhibitor-free RNA from complex samples with maximum binding capacity&nbsp;
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA MagBead Packs deliver high-integrity total transcripts without dependence on centrifuge spins or vacuum manifold.
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<h2>How to Choose a Magnetic Bead Total RNA Extraction Kit</h2>
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Automation Compatibility
Confirm that the kit supplier provides automation scripts for your specific platform. The Zymo Research Quick-DNA/RNA Viral MagBead kit and Thermo Fisher MagMAX-96 both offer downloadable instrument scripts for Tecan and Hamilton systems. Generic magnetic bead kits without published automation protocols require in-house script development, adding significant setup time.
Small RNA Retention
Bead kits that bind RNA &gt;=17 nt co-purify microRNAs alongside mRNA and rRNA from the same input. Kits specifying &gt;=200 nt retention exclude small RNAs and are not appropriate for miRNA expression panels. Check the size cutoff stated in the kit datasheet before selecting for non-coding RNA work.
Input Sample Type
Magnetic bead kits for total RNA support cell pellets, tissue homogenates, blood, bacteria, yeast, and fungi. For cell-free matrices (plasma, serum, nasal wash), switch to a viral-RNA or liquid-biopsy magnetic bead kit with included carrier RNA to maximize recovery from low-input, cell-free samples.
Elution Volume and Concentration
Standard magnetic bead kits elute into 50-100 ul; some protocols allow concentration into 20-50 ul, increasing RNA concentration for sensitive downstream applications. If downstream libraries require &gt;=200 ng/ul input, reduce elution volume accordingly and verify the kit supports this protocol variant.
gDNA Removal
Confirm whether the kit includes on-bead DNase I or requires a separate gDNA digestion step. For RT-qPCR and RNA-seq, omitting DNase treatment introduces a genomic DNA signal that inflates expression measurements.
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<h2>Specifications Context</h2>
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RNA purity targets from magnetic bead extractions match spin-column benchmarks: A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5. RNA integrity number (RIN) &gt;= 7 is recommended for RNA-seq library preparation; magnetic bead kits produce RIN values in the 8-10 range for fresh samples when lysis is performed immediately on ice. Bead-based workflows are cited in published automation protocols from genomics core facilities and are increasingly specified by NIH-funded projects requiring standardized sample processing across multi-site studies.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. to get automation-compatible mag bead selections today.

Protocol - HighPrep™ Total RNA Isolation Kit - iSWAB™

Magbio HighPrep Total RNA Isolation Kit-iSWAB™

HighPrep Total RNA Isolation Kit-iSWAB (ISTOR-R0) by MagBio Genomics — magnetic bead-based RNA extraction from 600 µL buccal swab lysate (MAWI iSWAB-RNA V2). Elutes 30–50 µL, OD260/280 1.8–2.0, DNase I included. RT-qPCR, RNA-seq, cDNA synthesis compatible.

Instrument

RNA Purity

HighPrep Total RNA Isolation Kit - iSWAB™ (5 preps)

HighPrep Total RNA Isolation Kit - iSWAB™ (50 preps)

HighPrep Total RNA  Isolation Kit - iSWAB™ (100 preps)

HighPrep Total RNA  Isolation Kit - iSWAB™  (400 preps)

HighPrep™ Total RNA Plus Kit - Protocol

MSDS

DataSheet

Magbio HighPrep Total RNA Plus Kit

HighPrep Total RNA Plus Kit by MagBio Genomics is a magnetic bead-based total RNA extraction kit for swab lysates, tissue (~30 mg), whole blood (200 µL), and cultured cells (1×10⁶) — 7-step protocol with included DNase treatment, OD260/280 purity 1.9–2.0, ≥30 µL elution, KingFisher Flex compatible. Available in 50-prep, 100-prep, and 400-prep formats.

Downstream application

HighPrep Total RNA Plus Kit (5 preps)

HighPrepTotal RNA Plus Kit (50 preps)

HighPrep Total RNA Plus Kit (100 preps)

HighPrep Total RNA Plus Kit (400 preps)

<h2>What are Magnetic Bead Viral RNA Extraction Kits?</h2>
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Magnetic bead viral RNA extraction kits purify viral RNA from cell-free biological matrices - plasma, serum, nasopharyngeal swabs, urine, saliva, blood, and cell culture supernatant - by binding viral nucleic acids to paramagnetic silica-coated beads during chaotropic lysis. A magnetic field captures bead-RNA complexes during wash steps, eliminating centrifugation, and purified viral RNA elutes in 20-50 ul of RNase-free water or elution buffer suitable for direct use in RT-qPCR, RT-LAMP, and next-generation sequencing. The 96-well plate format processes all samples in a single run on a compatible liquid-handling robot, making this the standard choice for surveillance programs, diagnostic batch processing, and clinical biobank workflows. Choose a magnetic bead viral RNA kit when daily sample volumes exceed 24 extractions per session, when automation infrastructure is available, or when viral loads are low and maximum sensitivity from a small elution volume is required.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Magbio HighPrep Viral RNA Kit KingFisher for seamless, automated viral RNA extraction using specialized superparamagnetic bead technology.</li>
<li>KingFisher-compatible magnetic bead workflows designed for high-throughput pathogen processing.</li>
<li>Automation-ready viral RNA purification solutions that reduce manual handling and improve laboratory efficiency.</li>
<li>Magnetic bead-based extraction systems for reliable recovery of viral nucleic acids from diverse sample types.</li>
<li>High-quality viral RNA isolation products suitable for RT-PCR, qPCR, sequencing, and molecular diagnostics applications.</li>
<li>Scalable pathogen extraction kits optimized for routine testing, surveillance, and research workflows.</li>
</ul>
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<h2>How to Choose a Magnetic Bead Viral RNA Extraction Kit</h2>
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Automation Platform Compatibility
Confirm the kit provides validated automation scripts for your instrument before purchase. Zymo Research Quick-DNA/RNA Viral MagBead kits support Tecan Fluent, Hamilton STAR, and Beckman Biomek; Thermo Fisher MagMAX Viral/Pathogen kits are validated on the KingFisher Flex and Duo systems. Generic bead kits without platform-specific scripts require in-house protocol development that adds weeks of validation time.
RNA-Only vs. DNA/RNA Co-Extraction
For pan-pathogen surveillance panels covering both RNA viruses (influenza, SARS-CoV-2, RSV) and DNA viruses (EBV, CMV, adenovirus, HPV), select a kit explicitly validated for DNA/RNA co-extraction. RNA-only kits use binding conditions that discriminate against dsDNA and will underperform on DNA viral targets.
Sample Input Volume and Matrix
Standard magnetic bead viral RNA kits support 100-200 ul of plasma, serum, or swab eluate per well. For low-viral-load samples where concentrating a larger starting volume is critical, verify the kit protocol for 500 ul input per well and confirm bead volume scales proportionally. Samples stabilized in Zymo DNA/RNA Shield can be directly loaded without an additional inactivation step.
Elution Volume
A 20-50 ul elution concentrates viral RNA from a standard 200 ul input by 4-10-fold relative to the original matrix, improving RT-qPCR sensitivity by 0.5-1.5 Ct. For sequencing applications, a 50-100 ul elution recovers more total RNA mass, which is more important than concentration for library input requirements.
Carrier RNA
Confirm the kit includes carrier RNA in the lysis buffer for low-titer specimens. For samples with expected viral copies &lt; 1x10^3/ml, carrier RNA supplementation is not optional - it is the difference between detectable and non-detectable results.
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<h2>Specifications Context</h2>
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Performance benchmarks: &gt;=70% RNA recovery measured by spike-in controls, eluate A260/A280 &gt;= 1.7, and Ct within 2 cycles of reference extraction. Magnetic bead-based extraction has been benchmarked against the QIAGEN QIAcube commercial platform and shown comparable RT-qPCR sensitivity for SARS-CoV-2 N-gene and E-gene targets, as reported in published peer-reviewed diagnostic studies. Zymo Research Quick-DNA/RNA Viral MagBead kits (R2140-E, R2141-E) hold Emergency Use Authorization and CE-IVD designation for diagnostic use, and are used in SARS-CoV-2 clinical workflows. Store extracted viral RNA at -70C to -80C; pre-extraction sample stabilization with DNA/RNA Shield enables ambient-temperature transport for up to 30 days without loss of RNA integrity.
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MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
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HighPrep Viral RNA Kit (KF Format)

Product Fact Sheet - HighPrep Viral RNA Kit

Magbio HighPrep Viral RNA Kit KingFisher

The HighPrep Viral RNA Kit KingFisher Format by MagBio Genomics extracts viral RNA from 200 µL of nasopharyngeal swabs, whole blood, serum, plasma, and saliva in 23 minutes on KingFisher™ Flex (MVP_2Wash_200_Flex program). No organic solvents required. Optimized for SARS-CoV-2 RNA isolation. Compatible with RT-qPCR, PCR, NGS, and viral load monitoring workflows.