PCR & qPCR Film

4" Soft Rubber Roller

4&quot; Soft Rubber Roller (RL-PLT-01) by MBP is a reusable hand-held sealing tool with a 4-inch (10.16 cm) wide soft rubber surface that applies even, consistent pressure across a full microplate in one pass. Ensures complete adhesion of any MBP plate sealing film — no bubbles or lifted edges. Single unit. Ships ambient.

Application

Material

Manufacturer

AeraSeal™ SealMate™ Rolls

AeraSeal™ SealMate™ Rolls (RSMB-2) by MBP provide gas-permeable, hydrophobic AeraSeal film in a roll format for faster plate sealing. Each roll contains 50 sheets separated by adhesive-free, perforated, color-coded red bands. 2 rolls/pk (100 sheets total). Non-sterile (RSMB-2) or sterile (RSMB-2-S). Rated &minus;20&deg;C to +80&deg;C. Ships ambient.

Temperature Range

AeraSeal™ SealMate™ Rolls, 2 Rolls/Pk, 50 Sheets/Roll

AeraSeal™ SealMate™ Rolls, 2 Rolls/Pk, 50 Sheets/Roll, Sterile

<h2 dir="ltr">What are PCR and qPCR sealing films?</h2>
 PCR and qPCR sealing films are optically clear microplate seals applied to 96- and 384-well PCR plates to create an airtight barrier through every thermal cycle, preventing evaporation, condensation, and cross-well contamination while still letting fluorescence pass through to the qPCR detector. Most are pressure-sensitive adhesive (PSA) or heat-activated films built on polypropylene or polyester (PET) with low-autofluorescence chemistry. Standard formats fit SBS sheets of approximately 137 &times; 80 mm. Buy from this category whenever the seal sits on a plate that will be thermally cycled or read in real time, not stored or incubated under simple lids. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Optical-grade qPCR sealing films for fluorescence-based detection</li>
<li dir="ltr" role="presentation">Standard adhesive PCR films for routine thermal cycling</li>
<li dir="ltr" role="presentation">96-well microplate sealing films for standard PCR workflows</li>
<li dir="ltr" role="presentation">384-well compatible sealing films for high-throughput assays</li>
<li dir="ltr" role="presentation">High-temperature resistant films for -40&deg;C to 120&deg;C cycling</li>
<li dir="ltr" role="presentation">Low-evaporation sealing films for reaction stability</li>
<li dir="ltr" role="presentation">DNase/RNase-free films for contamination-sensitive assays</li>
</ul>
&nbsp;
<h2 dir="ltr">How to choose PCR or qPCR sealing film</h2>
 Confirm true optical clarity if you read fluorescence Not every "clear" sealing film has enough optical clarity and low enough autofluorescence for real-time PCR; choose a film specifically rated for qPCR with high light transmittance and no autofluorescence so excitation and emission pass cleanly. Standard storage films can suppress the signal. Decide between adhesive (PSA) and heat-seal Pressure-sensitive adhesive films are applied with a roller or paddle at the bench and peel off; heat-seal films bond under a plate sealer at controlled temperature and pressure for the strongest barrier in high-throughput and automated qPCR. PSA suits flexible workflows; heat-seal suits volume. Match the plate, thermal cycler, and skirt Confirm the film fits 96- or 384-well plates from your supplier and seats properly under the heated lid of your thermal cycler &mdash; full-skirt, semi-skirt, and non-skirt plates have different sealing surfaces. Sheet size near 137 &times; 80 mm is the SBS norm. Check chemical and temperature resistance PCR plate sealers and thermal cyclers reach 105 &deg;C routinely, and some films are rated to 150 &deg;C; check that the film and adhesive withstand the cycler protocol and the master mix without softening, lifting at corners, or leaving residue after peeling.
&nbsp;
<h2 dir="ltr">Specifications context</h2>
 Quality qPCR films are defined by light transmittance (typically &ge; 90 %), absence of autofluorescence, evaporation rate (commonly &le; 3 %), thermal tolerance to about 105 &deg;C with high-temperature variants to 150 &deg;C, and a DNase/RNase- and human-DNA-free certification appropriate for amplification. Most are supplied as flat SBS-format sheets around 137 &times; 80 mm, packed 100 per box; some have perforated end tabs for one-handed peeling. As of 2026, they sit in a low-to-mid price tier, with heat-seal films at the higher end.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Connect with the MBP team today</a>&nbsp;to find the perfect optical or standard seal for your amplification workflow. Browse the wider&nbsp;<a href="https://mbpinc.net/consumables/sealing-films">sealing films range </a>for post-PCR archiving and reorder today via <a href="https://mbpinc.net/quickorder">Quick Order</a>.

DataSheet

AlumaSeal II™ PCR Plate Seals

AlumaSeal II™ (AF-100) by MBP stays flat during application with no roll-back. DNase/RNase-free, chemically resistant including DMSO, easily pierceable. Non-sterile AF-100 (100 seals/case) and sterile AFS-25 (&minus;80&deg;C to +120&deg;C, 50 films/pack). Ships ambient.

AF-100 Aluminum Foil Sealing Film for PCR Plates and Cold Storage 100 Seals/Case

AlumaSeal II™, Sterile, PCR, long-term storage, light sensitive assays and robotics, -80°C to +120°C, 50 Sealing Films/Pack

EZcap™ FoilStrips™, 6 Strips per Sheet, 1X8 Rows per Strip

EZcap™ FoilStrips™ (F2CS1X8300) by MBP are precision-cut aluminum foil strip seals for PCR 8-tube strips or single rows of 96-well PCR or deep-well storage plates. 6 strips/sheet, 1&times;8 rows/strip. Remove easily with no splash back. Single SKU. Ships ambient.

EZcap™ PCR FilmStrips™, 8 Strips per Sheet, 1X8 Rows per Strip, For sealing a PCR 8-tube strip or a single row of a 96-well plate, 400/Pack

EZcap™ PCR FilmStrips™ (US-CS1X8-400) by MBP are adhesive peel-and-stick alternatives to PCR strip-caps — no sore thumbs. 8 strips/sheet, 1&times;8 rows/strip; compatible with PCR 8-tube strips and single 96-well plate rows. 400 strips/pack. Ships ambient; single SKU.

FoilSeal™ RollMate™, Dispenser +1 Roll of FoilSeal™ Film, 500 Pre-Scored Sheets,

FoilSeal™ RollMate™ (F2-RL-500) by MBP bundles a reusable RollMate dispenser with one roll of FoilSeal™ aluminum foil plate sealing film pre-scored into 500 sheets. Faster and more economical than single-sheet film packs. For high-throughput PCR, qPCR, and light-sensitive assay plate sealing. Ships ambient.

SealPlate® ColorTab™

SealPlate® ColorTab™ (SP-IDA-10) by MBP adds color-coded plate identification to standard SealPlate seals. Each film has a colored end-tab with a colored dot and white writing area. Available in Assorted (SP-IDA-10, 50/case) and individual Blue, Green, Lavender, Orange, or Red colors (SP-IDB/G/L/O/R-100, 100/case each). Ships ambient.

SealPlate® ColorTab™, Assorted, 50/Cs

SealPlate® ColorTab™, Blue, 100/Cs

SealPlate® ColorTab™, Green, 100/Cs

SealPlate® ColorTab™, Lavender, 100/Cs

SealPlate® ColorTab™, Orange, 100/Cs

SealPlate® ColorTab™, Red, 100/Cs

SealPlate® MiniStrips™, 4 Strips per Sheet, 2X8 Rows per Strip, Sterile

SealPlate® MiniStrips™ (SPS-2X8-50) by MBP are sterile adhesive plate sealing strips for partial-plate sealing: 4 strips per sheet, each covering 2&times;8 rows (16 wells). Ideal for assays using partial microplates or requiring selective row sealing. Ships ambient; store at room temperature.

SealPlate® RollMate™, Dispenser +1 Roll of SealPlate Film, 500 Pre-Scored Sheets

SealPlate® RollMate™ (SP2-RL-500) by MBP includes a dispenser and 1 roll of pre-scored SealPlate film (500 sheets). Pre-scored roll film is a more economical and efficient alternative to single sheeted film for rapidly sealing microplates or racked test tubes. Single SKU. Ships ambient.

SealPlate® for General laboratory use

SealPlate® (100-SEAL-PLT) by MBP is a general-purpose adhesive plate sealing film that minimizes evaporation, prevents contamination, and eliminates the edge effect of plate lids. Rated &minus;40&deg;C to +120&deg;C. Non-sterile (100-SEAL-PLT) or sterile (STR-SEAL-PLT), 100 films/pack each. Ships ambient.

SealPlate®, General laboratory use, short-term storage, incubation and ELISA, -40°C to +120°C, 100 Sealing Films/Pack

SealPlate®, Sterile, General laboratory use, short-term storage, incubation and ELISA,Temperature Range: -40°C to +120°C, 100 Sealing Films/Pack

ThermalSeal RTS Optically Clear, Silicone Adhesive

ThermalSeal RTS™ (TSS-RTQ-100) by MBP is an optically clear, silicone-adhesive plate seal for qPCR, long-term storage, protein crystallography, and HPLC. Direct alternative to Thermofisher 4311971 MicroAmp Optical Adhesive Film. Available in 100-seal (TSS-RTQ-100), 25-film (TSS-RTQ-25), and sterile 50-film (TSS-RTQS-50, &minus;80&deg;C to +105&deg;C) formats. Ships ambient.

Optical Adhesive Films For PCR, Storage & Crystallization - Replaces 4311971 by Thermofisher 100 Seals/Case

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, 25 Sealing Films/Pack

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, Sterile, qPCR, long-term storage, protein crystallography & HPLC, -80°C to +105°C, 50 Sealing Films/Pack

ThermalSeal® 2™ and ThermalSeal® A™

ThermalSeal® 2™ and ThermalSeal® A™ (TS2-100 / TSA-100) by MBP feature a soft, extra thick, conformable adhesive for a very strong seal around raised wells. Listed for PCR, &minus;40&deg;C to +120&deg;C, 100 films/pack each. Ships ambient.