SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
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<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

AzuraQuant Green 1-Step qPCR Mix - Product Sheet

MSDS

User Manual

AzuraQuant™ Green 1-Step qPCR Mix HiRox

The AzuraQuant™ Green 1-Step qPCR Mix HiRox by Azura Genomics is AzuraQuant™ Green 1-Step qPCR Mix HiRox, 2× master mix + 20× AzuraSprint RT for one-step RT-qPCR directly from RNA. SYBR Green channel, HiRox for Applied Biosystems instruments. 20 µL reactions, −20°C storage, 18-month shelf life. Available 100–10,000 reaction packs. Available in 100 reactions (AZ-3501), 500 reactions (AZ-3505), 1,000 reactions (AZ-3510), and 5,000 reactions (AZ-3550).

Manufacturer

AzuraQuant Green 1-Step qRT-PCR Kit Hi-Rox 100 Reactions

AzuraQuant Green 1-Step qPCR Mix HiRox 500 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant™ Green 1-Step qPCR Mix LoRox

The AzuraQuant™ Green 1-Step qPCR Mix LoRox by Azura Genomics is a 2× one-step RT-qPCR master mix (low ROX) with a companion 20× thermostable AzuraSprint reverse transcriptase and non-inhibitory intercalating dye, enabling single-tube RNA quantification with post-amplification melt curve analysis on low-ROX compatible instruments. Available in 100 (AZ-3701), 500 (AZ-3705), 1,000 (AZ-3710), and 5,000 reactions (AZ-3750).

AzuraQuant Green 1-Step qRT-PCR Kit LoRox 100 reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 500 Reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 1000 Reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 5000 Reactions

AzuraQuant Green Fast qPCR Mix HiRox

AzuraQuant Green Fast qPCR Mix

AzuraQuant™ Green Fast qPCR Mix HiRox

AzuraQuant™ Green Fast qPCR Mix HiRox is available in the following variants: AZ-2001, AZ-2005, AZ-2020, AZ-2050.AzuraQuant™ Green Fast qPCR Mix HiRox (AZ-2001) by Azura Genomics is a 2× hot-start SYBR-channel qPCR master mix with a high-ROX passive reference dye, optimised for Applied Biosystems and QuantStudio platforms. Compatible with standard and fast cycling protocols. Available in 100, 500, 2,000, and 5,000 reaction sizes from MBP Inc.

AzuraQuant Green Fast qPCR Mix HiRox, 100 reactions

AzuraQuant Green Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Green Fast qPCR Mix HiRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix HiRox 5,000 reactions

AzuraQuant Green Fast qPCR Mix - Product Sheet

qPCR Reagent Compatibility Tool

AzuraQuant™ Green Fast qPCR Mix LoRox

The AzuraQuant™ Green Fast qPCR Mix LoRox by Azura Genomics is AzuraQuant™ Green Fast qPCR Mix LoRox, 2× master mix with Azura HS Taq, SYBR Green channel, low ROX. 20 µL standard reactions, fast-cycling protocol (95°C 2 min / 5 sec denaturation / 20–30 sec anneal-extension). Stored at −20°C, 18-month shelf life. Available in 100 reactions (AZ-2101), 500 reactions (AZ-2105), 2,000 reactions (AZ-2120), and 5,000 reactions (AZ-2150).

AzuraQuant Green Fast qPCR Mix LoRox 100 reactions

AzuraQuant Green Fast qPCR Mix LoRox 500 Reactions

AzuraQuant Green Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix LoRox 5000 reactions

Product Literature

AzuraView™ GreenFast qPCR Blue Mix HR

AZ-2401 by Azura Genomics — AzuraView GreenFast qPCR Blue Mix HR; 2X SYBR Green master mix; Azura HS Taq; inert blue tracer dye; increased resistance to freeze-thaw; compatible with all standard qPCR instruments; 100 reactions (1×1 mL).

AzuraView GreenFast qPCR Blue Mix HR 100 Reactions

AzuraView GreenFast qPCR Blue Mix HR 500 Reactions

AzuraView GreenFast qPCR Blue Mix HR 2,000 Reactions

AzuraView GreenFast qPCR Blue Mix HR 5,000 Reactions

AzuraView GreenFast qPCR Blue Mix - Product Sheet

AzuraView™ GreenFast qPCR Blue Mix LR

Azura Genomics AzuraView™ GreenFast qPCR Blue Mix LR: 2× intercalating dye mix with Azura HS Taq, blue tracer dye, low ROX. Standard and fast cycling. AZ-2301 (100), AZ-2305 (500), AZ-2320 (2,000), AZ-2350 (5,000 rxns).