Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

User Manual

Product Literature

MSDS

AzuraQuant Probe Fast qPCR Mix HiRox

AZ-2701 by Azura Genomics — AzuraQuant Probe Fast qPCR Mix HiRox; 2× probe-based mix; high ROX passive reference; TaqMan®, Scorpions®, molecular beacon compatible; antibody hot-start; standard and fast cycling; 100–5000 rxn formats.

Manufacturer

AzuraQuant Probe Fast qPCR Mix HiRox 100 reactions

AzuraQuant Probe Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 2,000 reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 5,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox

AZ-2801 by Azura Genomics: AzuraQuant™ Probe Fast qPCR Mix LoRox — 2× master mix for TaqMan, Scorpions, and molecular beacon probe assays on standard and fast cycling instruments. Low ROX passive reference. Antibody hot-start. 100–10,000 rxn packs.

AzuraQuant Probe Fast qPCR Mix LoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 500 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 5,000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox

AZ-2901 by Azura Genomics is a 2x ready-to-use probe-based qPCR master mix formulated without ROX passive reference dye. Compatible with TaqMan®, Scorpions®, and molecular beacon probes. Designed for instruments that do not require ROX normalization, including Bio-Rad CFX, Roche LightCycler, and Qiagen Rotor-Gene. Available in 100 to 10,000 reaction formats.

AzuraQuant Probe Fast qPCR Mix NoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix NoRox 2000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 5000 reactions

AzuraView Probe Fast qPCR Mix Blue - Product Sheet

AzuraView™ Probe Fast qPCR Mix Blue

AZ-2601B by Azura Genomics is an inhibitor-tolerant 2× master mix formulated for probe-based real-time qPCR, including TaqMan® and molecular beacon assays, with a blue inert tracer dye for visual confirmation of pipetting and compatibility with standard and fast-cycling thermal cyclers.