qPCR/Real-Time PCR

SYBR/Primer-Probe

<h2 dir="ltr"> What Is a SYBR/Primer-Probe Master Mix?</h2>
 A SYBR/Primer-Probe master mix is a 2x formulation positioned to support both dye-based (SYBR Green) and primer-probe (hydrolysis probe, TaqMan-style) detection chemistries, typically built on a common hot-start Taq, dNTP, MgCl2, and buffer backbone with formulation adjustments that accommodate either an intercalating dye or a labelled probe, depending on the assay. Choose this combined format when your lab runs both SYBR Green and probe-based assays and wants to standardise on fewer master mix products across the qPCR workflow.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="tosb50" data-start="110" data-end="247">One-step RT-qPCR kits that combine reverse transcription and real-time PCR in a single tube to streamline RNA quantification workflows.</li>
<li data-section-id="11s385w" data-start="248" data-end="363">Hot-start enzyme systems are designed to reduce non-specific amplification during reaction setup and thermal cycling.</li>
<li data-section-id="1sl7e5s" data-start="364" data-end="494">Probe-based detection chemistries for specific RNA target quantification in gene expression and pathogen detection applications.</li>
<li data-section-id="7bdacc" data-start="495" data-end="613">HiROX, LoROX, and NoROX formulation options to support compatibility with a wide range of real-time PCR instruments.</li>
<li data-section-id="19ytf2e" data-start="614" data-end="737">Optimized one-step workflows that reduce sample handling and help minimize contamination risk in high-throughput studies.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR/Primer-Probe Master Mix</h2>
 Assay Portfolio Mix Labs running a mix of SYBR Green gene expression assays and probe-based genotyping or pathogen-detection assays can benefit from standardising on a combined-compatible master mix line, reducing the number of distinct products to validate, store, and track across the facility. Reference Dye Configuration Confirm whether the specific product includes ROX or other passive reference dyes as standard, since probe-based assays in particular often rely on ROX for instrument normalisation, while some SYBR-only workflows may use ROX-free instruments. Validation Across Chemistries When adopting a combined-compatible master mix for the first time, validating performance separately for both a representative SYBR Green assay and a representative probe-based assay is recommended, since buffer optimisations that favour one chemistry slightly can sometimes trade off against the other. Primer and Probe Concentration Guidelines Typical primer-probe qPCR setups use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M; confirm the master mix manufacturer's recommended concentration ranges for both components when switching from a single-chemistry product. Throughput and Inventory Simplification For core facilities supporting multiple research groups with different assay types, a combined-compatible master mix can simplify inventory management and reduce the risk of using the wrong master mix for a given assay type. 
<h2 dir="ltr"> Specifications Context</h2>
 Standard real-time PCR master mixes are 2x concentrated and contain dNTPs, MgCl2, and DNA polymerase as their common backbone; a SYBR/primer-probe combined format extends this backbone with formulation choices intended to perform acceptably whether the researcher adds SYBR Green dye for dye-based detection or a labelled probe for probe-based detection. In probe-based setups, primer pairs are commonly used at a final concentration around 0.5 &micro;M with probes at 0.25-0.5 &micro;M, while SYBR-based protocols may additionally include a small amount of random hexamer primer in RT-qPCR contexts. Real-time PCR master mixes of this type are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-yield&nbsp;SYBR/Primer-Probe Master Mixes for your lab.

AzuraQuant Probe 1-Step qPCR Mix - Product Sheet

MSDS

User Manual

AzuraQuant Probe 1-Step qPCR Mix HiRox

AZ-3901 AzuraQuant Probe 1-Step qPCR Mix HiRox by Azura Genomics is a 2× RT-qPCR master mix with high ROX reference dye, compatible with TaqMan® and Scorpions® probes, 1 pg–1 µg RNA input, 20 µl reactions, stored at −20°C.

Manufacturer

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 500 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant Probe 1-Step qPCR Mix LoRox

AZ-4101 by Azura Genomics is the AzuraQuant Probe 1-Step qPCR Mix LoRox — a 2× hot-start RT-qPCR master mix with 20× AzuraSprint Reverse Transcriptase for one-tube RT-qPCR using TaqMan, Scorpions, or molecular beacon probes. Available from 100 to 10,000 reactions. LoRox formulation for low passive reference instruments.

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 500 reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 1,000 Reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 5,000 Reactions

AzuraQuant Probe 1-Step qPCR Mix NoRox

AZ-4301 by Azura Genomics is AzuraQuant™ Probe 1-Step qPCR Mix NoRox — a 2× RT-qPCR master mix containing Azura HS Taq and AzuraSprint thermostable RT. Compatible with TaqMan®, Scorpions®, and molecular beacon probes. Available in 100 to 10,000 reaction formats. No ROX passive reference dye.

AzuraQuant Probe 1-Step qPCR Mix No-Rox 100 Reactions

AzuraQuant Probe 1-Step qPCR Mix No-Rox 500 Reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 5,000 reactions

Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

Product Literature

AzuraQuant Probe Fast qPCR Mix HiRox

AZ-2701 by Azura Genomics — AzuraQuant Probe Fast qPCR Mix HiRox; 2× probe-based mix; high ROX passive reference; TaqMan®, Scorpions®, molecular beacon compatible; antibody hot-start; standard and fast cycling; 100–5000 rxn formats.

AzuraQuant Probe Fast qPCR Mix HiRox 100 reactions

AzuraQuant Probe Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 2,000 reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 5,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox

AZ-2801 by Azura Genomics: AzuraQuant™ Probe Fast qPCR Mix LoRox — 2× master mix for TaqMan, Scorpions, and molecular beacon probe assays on standard and fast cycling instruments. Low ROX passive reference. Antibody hot-start. 100–10,000 rxn packs.

AzuraQuant Probe Fast qPCR Mix LoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 500 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 5,000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox

AZ-2901 by Azura Genomics is a 2x ready-to-use probe-based qPCR master mix formulated without ROX passive reference dye. Compatible with TaqMan®, Scorpions®, and molecular beacon probes. Designed for instruments that do not require ROX normalization, including Bio-Rad CFX, Roche LightCycler, and Qiagen Rotor-Gene. Available in 100 to 10,000 reaction formats.

AzuraQuant Probe Fast qPCR Mix NoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix NoRox 2000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 5000 reactions

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

AzuraQuant Green 1-Step qPCR Mix - Product Sheet

AzuraQuant™ Green 1-Step qPCR Mix HiRox

AZ-3501 by Azura Genomics — AzuraQuant™ Green 1-Step qPCR Mix HiRox, 2× master mix + 20× AzuraSprint RT for one-step RT-qPCR directly from RNA. SYBR Green channel, HiRox for Applied Biosystems instruments. 20 µL reactions, −20°C storage, 18-month shelf life. Available 100–10,000 reaction packs.

AzuraQuant Green 1-Step qRT-PCR Kit Hi-Rox 100 Reactions

AzuraQuant Green 1-Step qPCR Mix HiRox 500 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant™ Green 1-Step qPCR Mix LoRox

AZ-3701 by Azura Genomics is a 2× one-step RT-qPCR master mix (low ROX) with a companion 20× thermostable AzuraSprint reverse transcriptase and non-inhibitory intercalating dye, enabling single-tube RNA quantification with post-amplification melt curve analysis on low-ROX compatible instruments.

AzuraQuant Green 1-Step qRT-PCR Kit LoRox 100 reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 500 Reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 1000 Reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 5000 Reactions

AzuraQuant Green Fast qPCR Mix HiRox

AzuraQuant Green Fast qPCR Mix

AzuraQuant™ Green Fast qPCR Mix HiRox

AzuraQuant™ Green Fast qPCR Mix HiRox (AZ-2001) by Azura Genomics is a 2× hot-start SYBR-channel qPCR master mix with a high-ROX passive reference dye, optimised for Applied Biosystems and QuantStudio platforms. Compatible with standard and fast cycling protocols. Available in 100, 500, 2,000, and 5,000 reaction sizes from MBP Inc.

AzuraQuant Green Fast qPCR Mix HiRox, 100 reactions

AzuraQuant Green Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Green Fast qPCR Mix HiRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix HiRox 5,000 reactions

AzuraQuant Green Fast qPCR Mix - Product Sheet

qPCR Reagent Compatibility Tool

AzuraQuant™ Green Fast qPCR Mix LoRox

AZ-2101 by Azura Genomics — AzuraQuant™ Green Fast qPCR Mix LoRox, 2× master mix with Azura HS Taq, SYBR Green channel, low ROX. 20 µL standard reactions, fast-cycling protocol (95°C 2 min / 5 sec denaturation / 20–30 sec anneal-extension). Stored at −20°C, 18-month shelf life. Available in 100–5,000 reaction packs.

AzuraQuant Green Fast qPCR Mix LoRox 100 reactions

AzuraQuant Green Fast qPCR Mix LoRox 500 Reactions

AzuraQuant Green Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix LoRox 5000 reactions

AzuraView™ GreenFast qPCR Blue Mix HR

AZ-2401 by Azura Genomics — AzuraView GreenFast qPCR Blue Mix HR; 2X SYBR Green master mix; Azura HS Taq; inert blue tracer dye; increased resistance to freeze-thaw; compatible with all standard qPCR instruments; 100 reactions (1×1 mL).

AzuraView GreenFast qPCR Blue Mix HR 100 Reactions

AzuraView GreenFast qPCR Blue Mix HR 500 Reactions

AzuraView GreenFast qPCR Blue Mix HR 2,000 Reactions

AzuraView GreenFast qPCR Blue Mix HR 5,000 Reactions

AzuraView GreenFast qPCR Blue Mix - Product Sheet

AzuraView™ GreenFast qPCR Blue Mix LR

AzuraView™ GreenFast qPCR Blue Mix LR (AZ-2301) by Azura Genomics — 2× ultra-sensitive intercalating dye master mix with Azura HS Taq, blue tracer dye, low ROX. Standard and fast cycling compatible, stable through 40 freeze-thaw cycles. Available from 100 to 10,000 reactions.