Accuris Instruments

1Kb DNA Ladders

<h2>What is a 1 kb DNA ladder?</h2>
&nbsp;
A 1 kb DNA ladder is a gel electrophoresis size standard containing a defined set of double-stranded DNA fragments spaced at or near 1-kilobase intervals, produced by digesting proprietary plasmids with restriction enzymes to generate typically 10 or more bands spanning 0.5 to 10 kb. One band, commonly at 3.0 kb, is supplied at higher concentration to serve as a bright reference band for rapid gel orientation. The 1 kb ladder is one of the most widely used size standards for routine molecular biology applications, covering the size range commonly encountered in PCR products, restriction digests, and cloned inserts.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">ZR 1 kb DNA Marker for gel electrophoresis analysis and routine DNA fragment sizing&nbsp;</li>
<li dir="ltr" role="presentation">Azura PureView&trade; 1kb DNA Ladder for the detection of DNA bands with high visibility in agarose gel systems</li>
<li dir="ltr" role="presentation">1kb Plus Opti-DNA Marker for cloning verification workflows and a wide range of DNA fragment analysis&nbsp;</li>
<li dir="ltr" role="presentation">1kb Opti-DNA Marker used in nucleic acid analysis and PCR for standard molecular weight reference&nbsp;</li>
<li dir="ltr" role="presentation">Accuris SmartCheck&trade; 1kb DNA Ladder for quality assessment of gel and DNA sizing with high precision</li>
<li dir="ltr" role="presentation">1 KB DNA Marker for routine DNA electrophoresis&nbsp;</li>
</ul>
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<h2>How to choose a 1 kb DNA ladder variant</h2>
&nbsp;
Choose a standard 1 kb ladder for routine PCR and restriction digest analysis
A standard 1 kb ladder with fragments spanning approximately 0.5 to 10 kb is suitable when most expected DNA fragments fall within this range, making it ideal for routine PCR verification and restriction digest analysis.
Choose a 1 kb Plus ladder when samples include smaller fragments below 500 bp
The 1 kb Plus format extends coverage down to 100 bp while maintaining the upper size range, making it useful when both small PCR products and larger DNA fragments need to be analyzed on the same gel.
Choose a 1 kb Extend ladder when samples include very large DNA fragments
The 1 kb Extend ladder expands coverage up to approximately 48.5 kb and is appropriate for large plasmids, BAC clones, and other high-molecular-weight DNA fragments analyzed on low-percentage agarose gels.
Use the recommended loading volume for accurate mass estimation
Loading the ladder according to the manufacturer's recommended amount helps ensure that any provided band mass references can be used for approximate comparison of DNA quantity based on staining intensity.
Confirm the tracking dye is compatible with your staining and imaging setup
Different ladder formulations use different tracking dyes. Some dyes may partially obscure nearby bands under UV visualization, while alternative formulations are designed to minimize interference and improve visibility across the entire ladder range.
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<h2>Specifications context</h2>
&nbsp;
Standard 1 kb DNA ladders are generated from defined DNA templates digested to produce fragments of known sizes. Enhanced-intensity reference bands are included to provide a readily identifiable orientation marker during gel analysis.
Ready-to-load 1 kb ladder formats with reduced-shadow tracking dyes are commonly used in routine agarose gel electrophoresis workflows because they simplify preparation and improve overall band visibility.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> to book high-quality 1Kb DNA Ladders for your lab today.

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 500 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 1000 reactions

Accuris qMAX™ Green One-Step RT-qPCR Kit

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 100 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 500 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 1000 reactions

Accuris qMAX™ Probe One-Step RT-qPCR Kit

40mM dNTP Mix, ready-to-use, 10mM/20μmol each, 0.5ml

40mM dNTP Mix, ready-to-use, 10mM/80μmol each, 2ml

dNTP Mix

100mM dNTP Set, dATP, dCTP, dGTP, dTTP, 250μl/25μmol each

100mM dNTP Set, dATP, dCTP, dGTP, dTTP, 1ml/100μmol each

dNTP Set

Accuris SmartCheck™ 50bp DNA Ladder, 500µl / 100 lanes

Accuris SmartCheck™ 50bp DNA Ladder, 5 x 500µl / 500 lanes

Accuris SmartCheck™ 100bp DNA Ladder, 500µl / 100 lanes

Accuris SmartCheck™ 100bp DNA Ladder, 5 x 500µl / 500 lanes

Accuris SmartCheck™ 1kb DNA Ladder, 500µl / 100 lanes

Accuris SmartCheck™ 1kb DNA Ladder, 5 x 500µl / 500 lanes

Accuris SmartCheck™ DNA Ladders

Accuris SmartCheck™ 1kb DNA Ladder

Accuris SmartCheck™ 1kb DNA Ladder (PR4100) by MBP covers 13 bands from 250&ndash;10,000 bp. Ready-to-use at 0.1 mg/ml, 5 &mu;l per lane, with loading buffer and tracking dyes included. Available in PR4100-100 (500 &mu;l / 100 lanes) and PR4100-500 (5 &times; 500 &mu;l / 500 lanes). Store at &minus;20&deg;C.

Protocol

Concentration

Tracking dye

Buffer formulation

Shipping and Storage

Production

Quality Control

Manufacturer

Accuris SmartCheck™ 1kb DNA Ladder, 500ul / 100 lanes

Accuris SmartCheck™ 1kb DNA Ladder, 5 x 500ul / 500 lanes

50bp DNA Ladders

<h2>What is a 50 bp DNA ladder?</h2>
&nbsp;
A 50 bp DNA ladder provides reference bands spaced at 50-base-pair intervals, most commonly covering 50 bp to 1000 bp (or up to 1500 bp in some extended formats), making it suitable for gel electrophoresis experiments where samples are very small DNA fragments that a 100 bp ladder cannot resolve accurately because 100 bp band spacing is too coarse. Common applications include microsatellite and STR genotyping, analysis of small restriction digest products, multiplex or nested PCR amplicons, and other short-fragment applications in the 50&ndash;500 bp range where precise size discrimination is needed.
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<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZR 50 bp DNA Marker for precise analysis of smaller DNA fragments&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Azura PureView&trade; 50 bp DNA Ladder for high-resolution gel results in electrophoresis
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Accuris SmartCheck&trade; 50bp DNA Ladder for size determination of PCR products
</li>
</ul>
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<h2>How to choose and use a 50 bp DNA ladder</h2>
&nbsp;
Use a 50 bp ladder when your fragments differ by less than 100 bp and are below 500 bp
The primary reason to use a 50 bp ladder over a 100 bp ladder is when your samples include fragments with size differences of less than 100 bp that need to be resolved, such as microsatellite alleles or closely-sized PCR amplicons from multiplex reactions.
Run small-fragment gels on 2&ndash;4% agarose or polyacrylamide for adequate resolution
High-percentage agarose (2&ndash;4%) or polyacrylamide gels are needed to resolve very small DNA fragments as distinct bands rather than all migrating together near the gel front, since standard 1% agarose does not provide sufficient resolution for fragments below approximately 200 bp.
Use a reference band at a convenient size for quick orientation
Many 50 bp ladders include a brighter reference band at a convenient, easy-to-remember size such as 250 bp or 500 bp, which serves as an anchor for identifying surrounding bands without counting from the well each time.
Confirm the 50 bp ladder's smallest band is detectable with your staining system
Very small DNA fragments, especially below 100 bp, can be difficult to detect or may run off the gel front quickly, and confirming that the ladder's smallest defined bands are visible under your staining and imaging setup helps ensure the full ladder range is usable.
Consider polyacrylamide gels for the highest resolution of fragments below 200 bp
Polyacrylamide gel electrophoresis provides substantially higher resolution than high-percentage agarose for fragments below 100&ndash;200 bp, making it the preferred format when single-base or very fine size discrimination is needed in this range, such as for SSR or STR genotyping.
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<h2>Specifications context</h2>
&nbsp;
Standard 50 bp DNA ladders typically cover from 50 bp to 1000 bp with bands at 50 bp intervals, and many include a brighter reference band at 250 bp or 500 bp for quick orientation. As of 2026, the 50 bp ladder remains the standard small-fragment size reference for microsatellite and STR genotyping workflows, whether on standard agarose or capillary electrophoresis platforms where defined size standards are loaded alongside samples.
<a href="https://mbpinc.net/contact-us">Contact the expert team of Molecular Biology Products Inc. (MBP)</a> and get premium-quality 50bp DNA ladders for your next experiment.

Accuris SmartCheck™ 50bp DNA Ladder

Accuris SmartCheck™ 50bp DNA Ladder (PR4005) by MBP is a ready-to-use small fragment DNA size standard with higher intensity reference bands and loading buffer included. Load 5 &mu;l per lane (100 lanes per 500 &mu;l vial). Available as PR4005-100 (100 lanes) or PR4005-500 (500 lanes). Ships ambient; store at &minus;20&deg;C.

Accuris SmartCheck™ 50bp DNA Ladder, 500ul / 100 lanes

Accuris SmartCheck™ 50bp DNA Ladder, 5 x 500ul / 500 lanes

Taq PCR Master Mixes

<h2 dir="ltr"> What Is a Taq PCR Master Mix?</h2>
 The 2x Taq PCR master mix format has become the standard for high-throughput molecular biology workflows because it combines reliability, convenience, and reproducibility in a single-tube format. By pre-optimising the concentrations of all PCR components, master mixes reduce pipetting steps and experimental variability compared to assembling reactions from individual components. This simplifies workflow setup and improves reproducibility. Select a formulation designed for your amplification needs.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr">High-Purity Enzymes: Versatile independent polymerases, such as the Azura&trade; and Accuris series, crafted for adaptable reaction adjustments and enhanced processivity with various DNA templates.</li>
<li dir="ltr">Full Reaction Solutions: All-inclusive systems such as the PCR Sure&trade; Kit that feature optimized dNTPs and buffers, offering a ready-to-use option for labs requiring dependable high-throughput performance and consistent outcomes.</li>
</ul>
<h2 dir="ltr"> How to Choose a Taq PCR Master Mix</h2>
 Standard vs. Dye-Containing Standard 2x master mixes require a separate loading dye addition before gel electrophoresis. Dye-containing master mixes include loading dye in the formulation, allowing direct gel loading after amplification &mdash; streamlining routine band-confirmation workflows. Component Pre-Optimisation A 2x Taq PCR master mix is a pre-formulated, double-concentrated solution containing Taq DNA polymerase, dNTPs (typically 200 &micro;M each at 1x), MgCl2, and PCR buffer at optimised concentrations &mdash; requiring only template and primer addition to set up a reaction. Throughput Scale High-throughput genotyping and colony screening benefit most from master mix consistency across multi-well plates, where individual component pipetting would introduce variability and time cost. Storage and Stability Master mixes are typically stable at &minus;20 &deg;C with multiple freeze-thaw cycles, though some dye-containing formulations may have slightly different stability profiles &mdash; check product-specific storage recommendations. Compatibility with Existing Protocols When switching from individual-component reactions to a master mix format, confirm that the pre-set MgCl2 and dNTP concentrations are compatible with your existing primer designs and templates, or adjust accordingly. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x Taq PCR master mix is set up by combining the master mix with template DNA and primers &mdash; typically at a 1:1 ratio of master mix to combined template/primer/water volume, achieving the final 1x working concentration. This format has become the standard for high-throughput molecular biology because it reduces pipetting steps and lot-to-lot variability across multi-well plate setups, important for reproducibility in genotyping and screening pipelines. Master mixes are typically stored at &minus;20 &deg;C with documented multiple freeze-thaw tolerance. When transitioning a protocol from individual-component PCR to a master mix format, running both formulations side-by-side on the same template and primer set is a useful validation step, confirming that the pre-set MgCl2 and dNTP concentrations in the master mix produce comparable yield and specificity to the previously optimised individual-component reaction before fully adopting the new format across a workflow. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
Enhance your amplification outcomes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team </a>now for a quote on our expert Taq Polymerase offerings.

Accuris High Fidelity, Hot Start Master Mix

Accuris High Fidelity DNA Polymerase & Master Mix

Accuris Taq Master Mix

Accuris Taq Master Mix (PR1001) by MBP is a ready-to-use Taq PCR master mix. Available in sample (PR1001-S), 200-reaction (PR1001-200), and 1,000-reaction (PR1001-1000) pack sizes. Ships on ice.

Accuris Taq Master Mix, 2X conc., sample, 5 reactions

Accuris Taq Master Mix, 2X conc., 200 reactions

Accuris Taq Master Mix, 2X conc., 1000 reactions

Accuris Taq DNA Polymerase 1000u (5u/µl)

Accuris Taq DNA Polymerase 6000u (5u/µl)

Accuris 2x Taq Master Mix 200 x 50µl Reactions

Accuris 2x Taq Master Mix 1000 x 50µl Reactions

Accuris 2x Taq Red Dye Master Mix 200 x 50µl Reactions

Accuris 2x Taq Red Dye Master Mix 1000 x 50µl Reactions

Accuris Taq DNA Polymerase & Master Mix

Accuris Taq Master Mix Red Dye

Accuris Taq Master Mix Red Dye (PR1001-R) by MBP is a ready-to-use 2X PCR master mix with enhanced Taq and a red loading dye for direct gel loading — no additional loading buffer needed. Ideal for routine PCR, genotyping, and colony PCR. Available in sample, 200-, and 1,000-reaction packs. Store at &minus;20&deg;C.

Accuris Taq Master Mix Red Dye, 2X conc., sample, 5 reactions

Accuris Taq Master Mix Red Dye, 2X conc., 200 reactions

Accuris Taq Master Mix Red Dye, 2X conc., 1000 reactions

Taq DNA Polymerase

<h2 dir="ltr">What Is Taq DNA Polymerase?</h2>
 Taq DNA polymerase, isolated from the thermophilic bacterium Thermus aquaticus, was the enzyme that made modern PCR possible. Its thermostability &mdash; retaining activity after repeated denaturation cycles at 94-95&deg;C &mdash; eliminated the need to add fresh enzyme after each cycle, a limitation of earlier PCR approaches using non-thermostable polymerases.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
High-Purity Enzymes: Versatile standalone polymerases, including Azura&trade; and Accuris series, available in flexible formats from 500 to 6000 units to suit both small-scale projects and high-volume industrial applications.
</li>
<li dir="ltr" aria-level="1">
Optimized Performance: Specialized formulations designed for superior processivity and flexible reaction tuning, allowing for high-yield synthesis across diverse and challenging DNA templates.
</li>
</ul>
<h2 dir="ltr"> How to Choose Taq DNA Polymerase</h2>
 Standard vs. Hot-Start Standard Taq is active immediately at room temperature, suitable for most routine genotyping and screening applications. Hot-start Taq formulations remain reversibly inactive until a high-temperature activation step, improving specificity for multiplex or low-abundance template reactions. Buffer System Compatibility Optimised 10x PCR buffers are formulated to complement the enzyme's activity profile &mdash; using mismatched buffer systems from other suppliers can affect yield and specificity, so matched buffer-enzyme pairs are recommended. MgCl2 Titration Needs MgCl2 concentration is one of the most influential variables in PCR specificity and yield. Separate MgCl2 solutions allow titration for templates or primer sets requiring optimisation beyond standard buffer concentrations. Concentration and Unit Format Enzyme is typically supplied in units (U) reflecting incorporation activity per unit time &mdash; confirm the unit concentration matches your typical reaction scale to avoid excessive dilution or waste. Application Scope Genotyping, colony screening after transformation, diagnostic band confirmation, and general routine amplification are the primary applications for standard and hot-start Taq DNA polymerase. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Taq DNA polymerase&rsquo;s thermostability &mdash; the ability to withstand repeated 94&ndash;95 &deg;C denaturation cycles without loss of activity &mdash; enabled practical PCR workflows based on a single thermostable enzyme. The enzyme lacks 3&prime;&rarr;5&prime; proofreading exonuclease activity, resulting in a higher error rate than high-fidelity polymerases (e.g., Phusion, Q5, KOD, Pfu-based systems), making it suitable for genotyping and screening but less appropriate for cloning- or sequencing-grade amplification.
Standard storage is at &minus;20 &deg;C, and many formulations are generally tolerant to multiple freeze&ndash;thaw cycles depending on buffer composition; lot-specific CoAs are available on request. In laboratories using both standard and hot-start Taq formulations, clear labelling and separation of storage conditions help prevent cross-use, particularly in assays where hot-start specificity is critical. A side-by-side validation on a known template when adopting a new Taq formulation can help establish expected amplification efficiency and specificity before routine use. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
Refine your amplification results&mdash;<a href="https://mbpinc.net/contact-us" title="contact the MBP team" style="color: #236fa1;">contact the MBP team</a> today for a quote on our professional Taq Polymerase solutions.

Accuris Taq Polymerase

Accuris Taq Polymerase (PR1000) by MBP is a modified standalone Taq enzyme with improved DNA-binding affinity, higher solubility, and greater template affinity for routine PCR. Available in sample, 500-, 1,000-, and 6,000-unit packs (PR1000-S / -500 / -1000 / -6000). Store at &minus;20&deg;C.

Accuris Taq Polymerase, sample, 50 units

Accuris Taq Polymerase, 500 units

Accuris Taq Polymerase, 1000 units

Accuris Taq Polymerase, 6000 units

cDNA Synthesis Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

Accuris qMAX Probe, No Rox qPCR Mix, Sample

Accuris qMAX Probe, No Rox qPCR Mix, 100 rxns (1 ml)

Accuris qMAX Probe, No Rox qPCR Mix, 500 rxns (5 ml)

Accuris qMAX Probe, No Rox qPCR Mix, 1000 rxns (10 ml)

Accuris qMAX Probe, Low Rox qPCR Mix, Sample

Accuris qMAX Probe, Low Rox qPCR Mix, 100 rxns (1 ml)

Accuris qMAX Probe, Low Rox qPCR Mix, 500 rxns (500 ml)

Accuris qMAX Probe, Low Rox qPCR Mix, 1000 rxns (10 ml)

Accuris qMAX Probe, High Rox qPCR Mix, Sample

Accuris qMAX Probe, High Rox qPCR Mix, 100 rxns (1 ml)

Accuris qMAX Probe, High Rox qPCR Mix, 500 rxns (5 ml)

Accuris qMAX Probe, High Rox qPCR Mix, 1000 rxns (10 ml)

Accuris qMAX™ Probe qPCR Mix

Accuris qMax cDNA Synthesis Kit

Accuris qMAX First Strand cDNA Synthesis Flex Kit, sample

Accuris qMAX First Strand cDNA Synthesis Flex Kit, 50 reactions

Accuris qMAX First Strand cDNA Synthesis Flex Kit, 200 reactions

Accuris qMAX™ First Strand cDNA Synthesis Flex Kit

Accuris qMAX First Strand cDNA Synthesis Flex Kit

Accuris qMAX First Strand cDNA Synthesis Flex Kit (PR2110) by MBP is a 4-component kit (Reverse Transcriptase, Buffer, oligo(dT), and random hexamer primers) for cDNA synthesis from 10 pg &ndash; 2.0 &mu;g RNA. Optimized for downstream qPCR. Available in sample, 50-reaction, and 200-reaction packs. Store at &minus;20&deg;C.

Accuris qMAX First Strand cDNA Synthesis Flex Kit, sample, 5 reactions

One-Step RT-qPCR Kits

<h2 dir="ltr"> What Are One-Step RT-qPCR Kits?</h2>
 A one-step RT-qPCR kit combines reverse transcription and real-time quantitative PCR in a single tube and buffer, using a temperature-activated reverse transcriptase paired with a hot-start Taq polymerase and a fluorescent detection chemistry - either an intercalating dye such as SYBR Green or a hydrolysis probe system such as TaqMan. The combined format allows room-temperature reaction setup, with reverse transcription and qPCR proceeding sequentially within the same thermal cycling protocol. Choose a SYBR Green one-step RT-qPCR kit for flexible, cost-effective RNA quantification across many targets; choose a probe-based one-step kit when higher specificity or multiplexing of several targets in one well is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="rd7qxi" data-start="780" data-end="912">Single-tube reverse transcription and real-time PCR workflows using one-step RT-qPCR kits from ZymoScript and Accuris qMAX</li>
<li data-section-id="1cvnwuk" data-start="913" data-end="1026">SYBR Green and hydrolysis probe-based detection chemistries for RNA quantification and gene expression analysis</li>
<li data-section-id="15d7q7t" data-start="1027" data-end="1119">One-step RT-qPCR master mix systems that reduce sample handling and streamline assay setup</li>
<li data-section-id="b3haux" data-start="1120" data-end="1195">Probe-based RT-qPCR kits suitable for target-specific detection workflows</li>
<li data-section-id="10mg35u" data-start="1196" data-end="1288">Green dye-based RT-qPCR kits for real-time amplification and quantification of RNA targets</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-qPCR Kit</h2>
 Detection Chemistry SYBR Green-based one-step RT-qPCR kits use an intercalating dye for broad, cost-effective quantification, with specificity driven by primer design. Probe-based one-step kits use TaqMan-style or other sequence-specific probes, offering higher specificity and multiplexing of multiple targets -- including via Scorpions primers or other probe formats -- in a single reaction. Reverse Transcriptase and Activation Mechanism Many one-step RT-qPCR kits use a temperature-activated or aptamer-based reverse transcriptase with dual enzyme-activity control, allowing room-temperature reaction setup without premature activity. Upon heating, both the reverse transcriptase and hot-start Taq polymerase reach their working states in a coordinated activation step. Buffer System Additives Specialised buffer additives -- such as ion combinations that promote specific primer annealing, or compounds that reduce annealing and extension times -- can improve specificity, sensitivity, and overall reaction speed, particularly useful for difficult templates or time-sensitive workflows. Internal Control Options Some one-step RT-qPCR kits offer an optional internal control RNA that can be co-amplified to monitor successful reverse transcription and amplification independently of the target signal, useful for troubleshooting and quality control in diagnostic-style workflows. Instrument Compatibility Confirm reference dye requirements (commonly ROX) and detection channel compatibility against your specific real-time PCR instrument before selecting a kit, since some formulations are offered with and without reference dye for compatibility across different instrument platforms. 
<h2 dir="ltr"> Specifications Context</h2>
 The combined one-step RT-qPCR reaction proceeds through reverse transcription at a temperature suited to the included reverse transcriptase (commonly in the 50-55 &deg;C range for engineered, higher-thermostability enzymes), followed by a polymerase activation step (commonly around 95 &deg;C) that simultaneously inactivates the reverse transcriptase and activates the hot-start Taq polymerase before standard qPCR cycling begins. Internal control RNA, when included, is typically detected in a separate fluorescence channel from the target, allowing simultaneous confirmation of reaction success and quantification of the target&nbsp;from the same well. One-step RT-qPCR kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs documenting performance and quality control are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized One-Step RT-PCR kits for your lab.

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 100 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 500 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 1000 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 100 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 500 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 1000 reactions

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 100 reactions

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 500 reactions

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 1000 reactions

Accuris qMAX™ Probe One-Step Multiplex RT-qPCR Kit

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

Accuris Probe One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

Accuris Probe One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

Accuris Probe Multiplex One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

Accuris Probe Multiplex One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

Accuris Bulk Packaging for High Throughput

Accuris qMAX Green One-Step RT-qPCR Kit

Accuris qMAX Green One-Step RT-qPCR Kit (PR2120) by MBP delivers RNA-to-qPCR in a single tube using qMax Green dye and Hot-Start Taq. Available in No ROX, Low ROX, and High ROX formulations in sample, 100-, 500-, and 1,000-reaction packs (12 variants total, PR2120-N-S through PR2120-H-1000). Store at &minus;20&deg;C.

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, sample 20 reactions

Accuris qMAX Green One-Step RT-qPCR Kit,No Rox, 100 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, 500 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, 1000 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, sample 20 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, sample 20 reactions

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

Accuris qMAX™ Green qPCR mix with blue tracking dye, No ROX, sample

Accuris qMAX™ Green qPCR mix with blue tracking dye, No ROX

Accuris qMAX™ Green qPCR mix with blue tracking dye, Low ROX, sample

Accuris qMAX™ Green qPCR mix with blue tracking dye, Low ROX

Accuris qMAX™ Green qPCR mix with blue tracking dye, High ROX, sample

Accuris qMAX™ Green qPCR mix with blue tracking dye, High ROX

Accuris qMAX™ Green qPCR Mix with Blue Tracking Dye

Accuris qMAX Green, with blue tracking dye qPCR Mix

Accuris qMAX Green qPCR Mix (PR2000) by MBP is a ready-to-use 2X master mix with Hot Start Taq, qMax Green dye, and blue tracking dye. Available in No ROX, Low ROX, and High ROX formulations in sample, 100-, 500-, and 1,000-reaction packs. 12 variants total (PR2000-N-S through PR2000-H-1000).

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, 100 reactions

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, 500 reactions

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, 1000 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, 100 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, 500 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, 1000 reactions

Accuris qMAX Green, with blue tracking dye, High Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Green, with blue tracking dye, High Rox qPCR Mix, 100 reactions

Accuris qMAX Green, with blue tracking dye,High Rox qPCR Mix, 500 reactions

Accuris qMAX Green, with blue tracking dye, High Rox qPCR Mix, 1000 reactions

One-Step RT-PCR Kits

<h2 dir="ltr">What Are One-Step RT-PCR Kits?</h2>
 A one-step RT-PCR kit combines reverse transcription and PCR amplification in a single reaction tube and buffer, using gene-specific primers throughout. The kit's enzyme mix typically pairs a reverse transcriptase -- commonly an M-MuLV-derived enzyme such as ProtoScript II or PrimeScript RT -- with a hot-start Taq DNA polymerase, sometimes including a proofreading component for higher-yield amplification.&nbsp; Choose a one-step kit for routine, single- or few-target RNA detection where simplicity and contamination control matter most; choose a two-step approach instead when one RNA sample needs to be tested against many different gene targets.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif; color: #000000;">One-step RT-PCR kits combining reverse transcription and PCR amplification in a single reaction workflow, including&nbsp;Azura 1-Step Ultra RT-PCR Kit</li>
<li style="color: #000000;">Standard one-step RT-PCR systems for RNA detection and target amplification, including One-Step RT-PCR Kit (RT0201-00) and Accuris One-Step RT-PCR Kit</li>
<li style="color: #000000;">Multiplex and probe-based one-step RT-qPCR-compatible systems, including Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit (No ROX)</li>
<li style="color: #000000;">High-performance one-step RT-PCR chemistry designed for robust RNA amplification, including the MegaFi One-Step RT-PCR Kit</li>
<li style="color: #000000;">Ready-to-use enzyme and buffer systems optimized for streamlined RT-PCR setup and reproducible results across RNA workflows</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-PCR Kit</h2>
 Enzyme Mix Composition Kits commonly combine an M-MuLV-derived reverse transcriptase with reduced RNase H activity and increased thermostability (e.g., ProtoScript II-type or PrimeScript-type enzymes) with a hot-start Taq polymerase. Some formulations add a proofreading polymerase component for higher-yield, more accurate end-point amplification. Hot-Start Activation Mechanism Most one-step kits use an antibody-modified or aptamer-based hot-start Taq that remains inactive at room temperature, dissociating during an initial high-temperature step (commonly around 94-95 &deg;C for several minutes) to simultaneously activate the polymerase and inactivate the reverse transcriptase before PCR cycling begins. Template Difficulty and Amplicon Length Reverse transcriptases with reduced RNase H activity and enhanced thermostability are better suited to GC-rich or highly structured RNA templates. Some one-step kits are validated for amplifying long transcripts -- up to approximately 9 kb in certain formulations -- relevant for full-length transcript or large-gene RT-PCR. Single-Tube Enzyme Compatibility Combining reverse transcriptase and Taq polymerase in one buffer historically risked mutual inhibition between the two enzyme classes; modern one-step formulations use optimised buffer systems and enzyme ratios specifically designed to avoid this interference, a key differentiator between basic and well-optimised kits. Detection Format End-point one-step RT-PCR kits (gel-based detection) suit qualitative presence/absence screening. For quantitative results, a one-step RT-qPCR kit with a fluorescent dye or probe system is the appropriate choice instead. 
<h2 dir="ltr"> Specifications Context</h2>
 The one-step format integrates the three RT-PCR steps -- reverse transcription, polymerase activation, and amplification -- into a single continuous reaction without opening the tube between steps, directly reducing contamination risk relative to workflows that transfer reaction products between vessels. Historically, combining reverse transcriptase and Taq polymerase in a shared buffer was reported to cause mutual enzyme inhibition and reduced sensitivity; current one-step kits address this through optimised buffer chemistry and enzyme selection, such as RNase H-minus reverse transcriptases paired with hot-start Taq formulations. One-step kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light, where fluorescent dyes are pre-included; lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-precision One-Step RT-PCR Kits for your lab.

Accuris qMAX Probe One-Step Multiplex  RT-qPCR Kit, No Rox

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit (PR2122-N) by MBP is specifically formulated for multiplex probe-based one-step RT-qPCR — simultaneous multi-target RNA quantification in a single tube. Hot-Start Taq, blue tracking dye, No ROX. Sample, 100-, 500-, and 1,000-reaction packs. Store at &minus;20&deg;C.

Accuris qMAX Probe One-Step Multiplex  RT-qPCR Kit, No Rox, sample 20 reactions

Accuris qMAX Probe One-Step Multiplex  RT-qPCR Kit, No Rox, 100 reactions

Accuris qMAX Probe One-Step Multiplex  RT-qPCR Kit, No Rox, 500 reactions

Accuris qMAX Probe One-Step Multiplex  RT-qPCR Kit, No Rox, 1000 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit

Accuris qMAX Probe One-Step RT-qPCR Kit (PR2121) by MBP delivers RNA-to-probe-based-qPCR in a single tube with Hot-Start Taq and blue tracking dye. Compatible with TaqMan™, Scorpions®, and molecular beacon probes. No ROX / Low ROX / High ROX formulations in sample, 100-, 500-, and 1,000-reaction packs (12 variants, PR2121-N-S through PR2121-H-1000). Store at &minus;20&deg;C.

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, sample 20 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 100 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 500 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 1000 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, sample 20 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, sample 20 reactions

Accuris qMAX Probe qPCR Mix

Accuris qMAX Probe qPCR Mix (PR2001) by MBP is a ready-to-use 2X probe-based qPCR master mix compatible with TaqMan™, Scorpions®, and molecular beacon probes. Hot-Start Taq, early Ct values, singleplex/multiplex compatible. No/Low/High ROX in sample, 100-, 500-, and 1,000-reaction packs (12 variants, PR2001-N-S through PR2001-H-1000). Store at &minus;20&deg;C.

Accuris qMAX Probe, No Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Probe, No Rox qPCR Mix, 100 reactions

Accuris qMAX Probe, No Rox qPCR Mix,  500 reactions

Accuris qMAX Probe No Rox qPCR Mix, 1000 reactions

Accuris qMAX Probe, Low Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Probe, Low Rox qPCR Mix, 100 reactions

Accuris qMAX Probe, Low Rox qPCR Mix, 500 reactions

Accuris qMAX Probe, Low Rox qPCR Mix, 1000 reactions

Accuris qMAX Probe, High Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Probe, High Rox qPCR Mix, 100 reactions

Accuris qMAX Probe, High Rox qPCR Mix, 500 reactions

Accuris qMAX Probe, High Rox qPCR Mix, 1000 reactions

Accuris qMAX cDNA Synthesis Kit

Accuris qMAX cDNA Synthesis Kit (PR2100-C) by MBP is a 2-tube format cDNA synthesis kit for 4 pg to 0.5 &mu;g input RNA. Available in sample (PR2100-C-S), 25-, 100-, and 250-reaction packs. A First Strand cDNA Synthesis Flex Kit (10 pg to 2.0 &mu;g) is also available on the same page.