qPCR/Real-Time PCR

One-Step RT-qPCR Kits

<h2 dir="ltr"> What Are One-Step RT-qPCR Kits?</h2>
 A one-step RT-qPCR kit combines reverse transcription and real-time quantitative PCR in a single tube and buffer, using a temperature-activated reverse transcriptase paired with a hot-start Taq polymerase and a fluorescent detection chemistry - either an intercalating dye such as SYBR Green or a hydrolysis probe system such as TaqMan. The combined format allows room-temperature reaction setup, with reverse transcription and qPCR proceeding sequentially within the same thermal cycling protocol. Choose a SYBR Green one-step RT-qPCR kit for flexible, cost-effective RNA quantification across many targets; choose a probe-based one-step kit when higher specificity or multiplexing of several targets in one well is required.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="rd7qxi" data-start="780" data-end="912">Single-tube reverse transcription and real-time PCR workflows using one-step RT-qPCR kits from ZymoScript and Accuris qMAX</li>
<li data-section-id="1cvnwuk" data-start="913" data-end="1026">SYBR Green and hydrolysis probe-based detection chemistries for RNA quantification and gene expression analysis</li>
<li data-section-id="15d7q7t" data-start="1027" data-end="1119">One-step RT-qPCR master mix systems that reduce sample handling and streamline assay setup</li>
<li data-section-id="b3haux" data-start="1120" data-end="1195">Probe-based RT-qPCR kits suitable for target-specific detection workflows</li>
<li data-section-id="10mg35u" data-start="1196" data-end="1288">Green dye-based RT-qPCR kits for real-time amplification and quantification of RNA targets</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-qPCR Kit</h2>
 Detection Chemistry SYBR Green-based one-step RT-qPCR kits use an intercalating dye for broad, cost-effective quantification, with specificity driven by primer design. Probe-based one-step kits use TaqMan-style or other sequence-specific probes, offering higher specificity and multiplexing of multiple targets -- including via Scorpions primers or other probe formats -- in a single reaction. Reverse Transcriptase and Activation Mechanism Many one-step RT-qPCR kits use a temperature-activated or aptamer-based reverse transcriptase with dual enzyme-activity control, allowing room-temperature reaction setup without premature activity. Upon heating, both the reverse transcriptase and hot-start Taq polymerase reach their working states in a coordinated activation step. Buffer System Additives Specialised buffer additives -- such as ion combinations that promote specific primer annealing, or compounds that reduce annealing and extension times -- can improve specificity, sensitivity, and overall reaction speed, particularly useful for difficult templates or time-sensitive workflows. Internal Control Options Some one-step RT-qPCR kits offer an optional internal control RNA that can be co-amplified to monitor successful reverse transcription and amplification independently of the target signal, useful for troubleshooting and quality control in diagnostic-style workflows. Instrument Compatibility Confirm reference dye requirements (commonly ROX) and detection channel compatibility against your specific real-time PCR instrument before selecting a kit, since some formulations are offered with and without reference dye for compatibility across different instrument platforms. 
<h2 dir="ltr"> Specifications Context</h2>
 The combined one-step RT-qPCR reaction proceeds through reverse transcription at a temperature suited to the included reverse transcriptase (commonly in the 50-55 &deg;C range for engineered, higher-thermostability enzymes), followed by a polymerase activation step (commonly around 95 &deg;C) that simultaneously inactivates the reverse transcriptase and activates the hot-start Taq polymerase before standard qPCR cycling begins. Internal control RNA, when included, is typically detected in a separate fluorescence channel from the target, allowing simultaneous confirmation of reaction success and quantification of the target&nbsp;from the same well. One-step RT-qPCR kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs documenting performance and quality control are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized One-Step RT-PCR kits for your lab.

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 500 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 1000 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 100 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 500 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 1000 reactions

Accuris qMAX™ Green One-Step RT-qPCR Kit

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 100 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 500 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 1000 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 100 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 500 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 1000 reactions

Accuris qMAX™ Probe One-Step RT-qPCR Kit

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 100 reactions

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 500 reactions

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 1000 reactions

Accuris qMAX™ Probe One-Step Multiplex RT-qPCR Kit

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

Accuris Probe One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

Accuris Probe One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

Accuris Probe Multiplex One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

Accuris Probe Multiplex One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

Accuris Bulk Packaging for High Throughput

Accuris qMAX Green One-Step RT-qPCR Kit

Accuris qMAX Green One-Step RT-qPCR Kit (PR2120) by MBP delivers RNA-to-qPCR in a single tube using qMax Green dye and Hot-Start Taq. Available in No ROX, Low ROX, and High ROX formulations in sample, 100-, 500-, and 1,000-reaction packs (12 variants total, PR2120-N-S through PR2120-H-1000). Store at &minus;20&deg;C.

Manufacturer

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, sample 20 reactions

Accuris qMAX Green One-Step RT-qPCR Kit,No Rox, 100 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, 500 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, 1000 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, sample 20 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, sample 20 reactions

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

Accuris qMAX™ Green qPCR mix with blue tracking dye, No ROX, sample

Accuris qMAX™ Green qPCR mix with blue tracking dye, No ROX

Accuris qMAX™ Green qPCR mix with blue tracking dye, Low ROX, sample

Accuris qMAX™ Green qPCR mix with blue tracking dye, Low ROX

Accuris qMAX™ Green qPCR mix with blue tracking dye, High ROX, sample

Accuris qMAX™ Green qPCR mix with blue tracking dye, High ROX

Accuris qMAX™ Green qPCR Mix with Blue Tracking Dye

Accuris qMAX Green, with blue tracking dye qPCR Mix

Accuris qMAX Green qPCR Mix (PR2000) by MBP is a ready-to-use 2X master mix with Hot Start Taq, qMax Green dye, and blue tracking dye. Available in No ROX, Low ROX, and High ROX formulations in sample, 100-, 500-, and 1,000-reaction packs. 12 variants total (PR2000-N-S through PR2000-H-1000).

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, 100 reactions

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, 500 reactions

Accuris qMAX Green, with blue tracking dye, No Rox qPCR Mix, 1000 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, 100 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, 500 reactions

Accuris qMAX Green, with blue tracking dye, Low Rox qPCR Mix, 1000 reactions

Accuris qMAX Green, with blue tracking dye, High Rox qPCR Mix, sample, 20 reactions

Accuris qMAX Green, with blue tracking dye, High Rox qPCR Mix, 100 reactions

Accuris qMAX Green, with blue tracking dye,High Rox qPCR Mix, 500 reactions

Accuris qMAX Green, with blue tracking dye, High Rox qPCR Mix, 1000 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit

Accuris qMAX Probe One-Step RT-qPCR Kit (PR2121) by MBP delivers RNA-to-probe-based-qPCR in a single tube with Hot-Start Taq and blue tracking dye. Compatible with TaqMan™, Scorpions®, and molecular beacon probes. No ROX / Low ROX / High ROX formulations in sample, 100-, 500-, and 1,000-reaction packs (12 variants, PR2121-N-S through PR2121-H-1000). Store at &minus;20&deg;C.

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, sample 20 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 100 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 500 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 1000 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, sample 20 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, sample 20 reactions

Accuris qMAX Probe, No Rox qPCR Mix, Sample

Accuris qMAX Probe, No Rox qPCR Mix, 100 rxns (1 ml)

Accuris qMAX Probe, No Rox qPCR Mix, 500 rxns (5 ml)

Accuris qMAX Probe, No Rox qPCR Mix, 1000 rxns (10 ml)

Accuris qMAX Probe, Low Rox qPCR Mix, Sample

Accuris qMAX Probe, Low Rox qPCR Mix, 100 rxns (1 ml)

Accuris qMAX Probe, Low Rox qPCR Mix, 500 rxns (500 ml)

Accuris qMAX Probe, Low Rox qPCR Mix, 1000 rxns (10 ml)

Accuris qMAX Probe, High Rox qPCR Mix, Sample

Accuris qMAX Probe, High Rox qPCR Mix, 100 rxns (1 ml)

Accuris qMAX Probe, High Rox qPCR Mix, 500 rxns (5 ml)

Accuris qMAX Probe, High Rox qPCR Mix, 1000 rxns (10 ml)

Accuris qMAX™ Probe qPCR Mix

Accuris qMAX Probe qPCR Mix

Accuris qMAX Probe qPCR Mix (PR2001) by MBP is a ready-to-use 2X probe-based qPCR master mix compatible with TaqMan™, Scorpions®, and molecular beacon probes. Hot-Start Taq, early Ct values, singleplex/multiplex compatible. No/Low/High ROX in sample, 100-, 500-, and 1,000-reaction packs (12 variants, PR2001-N-S through PR2001-H-1000). Store at &minus;20&deg;C.