Agarose

<h2>What is agarose for gel electrophoresis?</h2>
&nbsp;
Agarose is a linear polysaccharide derived from agar that, when dissolved in hot buffer and allowed to cool, forms a porous gel matrix whose pore size is determined by the agarose concentration. DNA and RNA molecules migrate through this matrix under an electric field at a rate inversely related to their size, allowing fragments of different lengths to be separated and visualized as distinct bands.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Azura Agarose for routine electrophoresis of nucleic acids with low electroendosmosis (EEO) performance</li>
<li dir="ltr" role="presentation">Benchmark Agarose for PCR product analysis and high-quality nucleic acid separation</li>
<li dir="ltr" role="presentation">Benchmark Specialty Agarose for specialized gel applications requiring enhanced resolution and performance control</li>
<li dir="ltr" role="presentation">Standard Agarose for routine use in gel electrophoresis workflows</li>
<li dir="ltr" role="presentation">EZ Pack&trade; Agarose Tablets for agarose concentration control and convenient gel preparation&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose agarose type and concentration</h2>
&nbsp;
Use standard agarose at 0.7&ndash;1% for routine PCR and restriction digest checks on larger fragments
A 0.7&ndash;1% standard agarose gel is commonly used for routine PCR product verification and restriction digest analysis when fragments are generally in the 1&ndash;10 kb size range.
Use 1.5&ndash;2% standard agarose for resolving smaller fragments in the 200 bp&ndash;2 kb range
Higher agarose concentrations from 1.5% to 2% are appropriate for resolving smaller PCR products, SNP genotyping amplicons, or restriction digests where size differences below 500 bp need to be clearly distinguished.
Use high-resolution agarose at 2&ndash;4% for very small fragments or close sizing
High-resolution agarose formulations can differentiate closely sized DNA fragments in the 50&ndash;500 bp range more effectively than standard agarose, making them useful for applications such as microsatellite or STR analysis.
Choose low-melting-point agarose when you need to recover DNA from gel slices
Low-melting-point (LMP) agarose can be melted at approximately 65&deg;C after electrophoresis, allowing DNA-containing gel slices to be processed more easily for downstream enzymatic reactions or purification workflows.
Confirm agarose purity (EEO grade) for sensitive applications
Electro-endosmosis (EEO) reflects agarose purity. Low-EEO agarose supports more consistent DNA migration and helps minimize contaminants that may interfere with downstream PCR amplification, restriction digestion, or other enzymatic applications.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Standard agarose gels are typically prepared by dissolving agarose powder in TAE or TBE buffer through heating, followed by cooling to approximately 55&deg;C before pouring into casting trays. Higher-concentration gels solidify more rapidly and generally benefit from prompt casting.
As of 2026, low-EEO agarose grades remain the preferred choice for sensitive applications such as Southern blotting, gel extraction followed by PCR, and restriction enzyme digestion workflows where contaminants can inhibit downstream reactions.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> and get premium agarose gel for your research today.

Benchmark Agarose LE (Molecular Biology Grade)

Benchmark Agarose LE (A1705) by MBP is a low EEO molecular biology-grade agarose powder for standard DNA and RNA gel electrophoresis. Compatible with TAE/TBE buffers and all common gel stains. Available in three pack sizes: A1705, A1701, and A1700. Stores at room temperature. Ships ambient.

Manufacturer

Benchmark Agarose LE, 500g

Benchmark Agarose LE, 100g

Benchmark Agarose LE, 25g

Benchmark Specialty Agarose

Benchmark Specialty Agarose (A1801-LM) by MBP is a specialty agarose available in three variants: A1801-LM, A1801-31, and A1801-HR. Contact MBP for variant-specific information.

Benchmark Agarose LM, Low Melt, 100g

Benchmark Agarose 3:1, 100g

Benchmark Agarose HR, PCR Grade for DNA fragments between 20 to 800bp, 100g

EZ Pack™ Agarose Tablets

EZ Pack™ Agarose Tablets (A2505) by MBP provide pre-measured molecular biology-grade agarose in tablet form for fast, reproducible, spill-free gel preparation — no weighing needed. Simply dissolve in TAE or TBE buffer by microwave. Available in A2505 and A2501 pack sizes. Stores at room temperature.