Immortalized Cells

Thawing Protocol

Cell Line Catalog (Volume 2)

Subculturing Protocol

Freezing Protocol

T2610 Zebrafish Testicular Feeder Cells (ZtA6-11)

T2610 Zebrafish Testicular Feeder Cells (ZtA6-11) are the eleventh clone of the ZtA6 zebrafish testicular feeder cell series, supporting spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2611 Zebrafish Testicular Feeder Cells (ZtA6-12)

T2611 Zebrafish Testicular Feeder Cells (ZtA6-12) are the twelfth clone of the ZtA6 series from albino zebrafish testis, requiring PriGrow XIII complex medium, Accutase passaging, gelatin coating, 38&deg;C/no CO2 incubation, and mitomycin C inactivation for feeder use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

<h2>What Are DNA-Modifying Enzymes?</h2>
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DNA-modifying enzymes catalyze defined chemical changes to DNA molecules: T4 DNA Ligase seals nicks and joins fragments; T4 Polynucleotide Kinase (PNK) transfers a phosphate from ATP to 5'-OH termini; alkaline phosphatase removes 5'-phosphate groups to block self-ligation; Klenow fragment fills in 5' overhangs and removes 3' overhangs to generate blunt ends; DNA methyltransferases add methyl groups to adenine or cytosine residues for epigenetic studies or protection from restriction digestion. These enzymes are distinct from PCR polymerases (which amplify DNA) and restriction enzymes (which cleave at specific sequences). Use DNA-modifying enzymes any time you need to alter DNA end chemistry, join fragments, or protect sequences from downstream enzymatic cleavage.
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<h2 dir="ltr">What you will find:</h2>
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Thermolabile UDG: An essential "cleanup" enzyme that removes carryover contamination in PCR by breaking down uracil-containing DNA, thus guaranteeing your results stay clear of artifact-induced sequence bias.
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T4 DNA Ligase: The crucial molecular adhesive, designed for the swift and efficient connection of blunt or cohesive-end DNA pieces in cloning and NGS adapter ligation.
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T4 Gene 32 Protein: A unique single-stranded DNA binding protein that maintains open templates, greatly enhancing the efficiency of enzymes in challenging amplification and recombination tests.
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<h2>How to Choose DNA-Modifying Enzymes</h2>
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Ligation format
T4 DNA Ligase is the standard choice for cohesive-end and blunt-end ligation in cloning. For high-throughput library prep, choose ligase formulations optimized for 25 degrees C or room-temperature reaction to reduce protocol time. E. coli DNA Ligase uses NAD+ (not ATP) and is preferred for nick sealing in nick-translation reactions.
End repair requirements
Klenow fragment (3' to 5' exo-) fills 5' overhangs and removes 3' extensions to generate blunt ends required for blunt-end cloning or adaptor ligation; its 3'-&gt;5' exonuclease-minus version is used in NGS A-tailing steps to add a single 3'-dA overhang. T4 DNA Polymerase has stronger 3'-&gt;5' exonuclease activity than Klenow and is preferred for end polishing of PCR products with heterogeneous ends.
Phosphorylation/dephosphorylation
Use T4 PNK to add 5'-phosphates to PCR products or synthetic oligonucleotides before blunt-end ligation. Use alkaline phosphatase (CIP or SAP) on linearized vectors to prevent self-ligation; SAP is preferred when heat-inactivation at 65 degrees C is needed before downstream steps. Combine dephosphorylation and ligation in the same tube only after inactivating the phosphatase.
Methylation and protection
CpG methyltransferases and dam/dcm methylases protect recognition sequences from restriction cleavage or enable methylation-sensitive mapping. Dam methylase (methylates GATC sequences) and Dcm methylase (methylates CCWGG) are used to prepare templates resistant to DpnI digestion in cloning workflows where DpnI is used to remove template plasmid.
Purity certification
For RNA co-purification or RNA-sensitive workflows, confirm all DNA-modifying enzymes carry RNase-free certification. ABM lot-release QC includes RNase and DNase contamination tests; certificates of analysis are available on request through MBP.
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<h2>Specifications Context</h2>
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T4 DNA Ligase activity is defined as the amount of enzyme catalyzing 50% ligation of HindIII-cut lambda DNA fragments in 30 minutes at 16 degrees C in a 20 microliter reaction; 1 Weiss unit equals approximately 60 cohesive-end units. Optimal blunt-end ligation uses PEG 4000 at 15% (w/v) as a molecular crowding agent and runs at 25 degrees C to increase effective enzyme concentration. Streamlined NGS library prep workflows combine end repair, A-tailing, and adaptor ligation into a single-tube step, reducing total hands-on time to under 1 hour.&nbsp;
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Seize control of your DNA processes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now for a quote on our premium DNA-altering enzymes.

DNA-modifying enzymes

DataSheet

MSDS

T4 DNA Ligase

Unit Quantity

Concentration

Format General

Reaction Definition

Storage Condition

Shipping Condition

T4 Gene 32 Protein

Note

Primary Cells

T4000 Human Primary Subcutaneous Adipocytes

T4000 Human Primary Subcutaneous Adipocytes by ABM are mature differentiated adipocytes from normal human adipose tissue (5×10⁵ cells/1.0 ml, BSL-II), confirmed by oil red O and Nile red staining. Seeding density 10,000–15,000 cells/cm²; requires PriCoat™ T25 Flasks and PriGrow TM4000 medium. Research use only; MTA required.

T4002 Human Primary Subcutaneous White Preadipocytes

T4002 Human Primary Subcutaneous White Preadipocytes — seeding 5,000/cm&sup2;; do NOT centrifuge at thawing; TM080 Gentle Dissociation Solution required for subculture (trypsin-sensitive); 220&times;g &times; 3 min. Differentiate with TM005. For adipogenesis, obesity, Type 2 diabetes, hyperlipidemia, and breast cancer adipose TME research. Supplied by ABM as 5&times;105 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T4003 Human Primary Alveolar Cells-Type 1

T4003 Human Primary Alveolar Cells-Type 1 express CK-18 and CK-19, 5,000/cm&sup2;, 36&ndash;48h PDT, 3&ndash;6 passages at 1:2, for alveolar blood-air barrier, VILI/ARDS, respiratory pathogen infection, pulmonary drug delivery, and alveolar injury/repair research. Supplied by ABM as 5&times;105 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T4005 Human Primary Tracheal Fibroblasts

T4005 Human Primary Tracheal Fibroblasts by ABM — cryopreserved, normal airway (trachea), 5×10⁵ cells/1.0 ml, BSL-II. MTA required. Research use only.

T4006 Human Primary Bronchial Epithelial Cells

T4006 Human Primary Bronchial Epithelial Cells express cytokeratin, seeded at 10,000&ndash;15,000/cm&sup2;, for bronchial airway barrier function, SARS-CoV-2/influenza/RSV infection, asthma allergen response, COPD-related injury, and inhaled drug screening. Supplied by ABM as 5&times;105 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T4007 Human Primary Tracheal Epithelial Cells

T4007 Human Primary Tracheal Epithelial Cells by ABM — cryopreserved, normal airway tissue, adherent, epithelial-like, 5×10⁵ cells/1.0 ml, BSL-II. Human Epithelial Cell Growth Medium Kit (TM106). MTA required. Research use only.

T4008 Human Primary Small Airway Epithelial Cells

T4008 Human Primary Small Airway Epithelial Cells by ABM — cryopreserved, normal airway tissue, adherent, polygonal, 5×10⁵ cells/1.0 ml, BSL-II. PriGrow X Series Medium (TM4008). MTA required. Research use only.

T4009 Human Primary Pulmonary Fibroblasts

T4009 Human Primary Pulmonary Fibroblasts are seeded at 3,500&ndash;7,000/cm&sup2; for lung ECM homeostasis, IPF myofibroblast differentiation, anti-fibrotic drug screening (pirfenidone/nintedanib-like), COPD fibroblast remodeling, and TGF-&beta; signaling research. Supplied by ABM as 5&times;105 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.