<h2 dir="ltr">What Are Cre-Inducible DIO Lentiviral Vectors?</h2>
 Conditional gene expression systems restrict transgene activation to defined cell populations &mdash; indispensable when whole-tissue expression would confound experimental interpretation or produce toxicity. DIO (Double-floxed Inverted Open reading frame) architectures place the gene of interest in an inverted, non-coding orientation until Cre recombinase flips it into the correct sense orientation for transcription.&nbsp;Conditional expression studies require precise genetic control. Choose a DIO vector compatible with your experimental model.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Lenti-CMV-DIO-copGFP-SV40-Puro Vectors: Feature copGFP fluorescent tracking and a puromycin resistance marker for the selection of stable cells.</li>
<li>Blank DIO-SV40-copGFP Vectors: Feature a copGFP reporter designed for cell-specific gene insertion and expression.</li>
<li>Lenti-CMV-DIO-Blank-SV40-Puro Vectors: Feature an empty DIO expression plasmid with SV40-driven puromycin resistance for reliable experimental normalization.</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a DIO Lentiviral Vector</h2>
 Insert Size AAV DIO vectors are limited to expression cassettes of approximately 4.2 kb. Lentiviral DIO vectors support cassettes up to ~10 kb, enabling delivery of large genes &mdash; including full-length membrane receptors, ion channels, and fluorescent fusion constructs &mdash; that cannot fit in an AAV genome. Cre Delivery Method Cre can be delivered by co-injection of a Cre-expressing lentiviral or AAV vector, stereotactic injection of DIO LV into a transgenic Cre-driver mouse line, or plasmid co-transfection of Cre in cell culture experiments. Permanence of Activation Once Cre flips the ORF into sense orientation, one site from each loxP pair is excised, leaving heterotypic lox sites that cannot undergo further Cre-mediated recombination &mdash; the transgene is permanently locked ON and heritably expressed in daughter cells. Validation Before In Vivo Use Co-transfection of the DIO vector with a Cre expression plasmid in HEK293T cells confirms efficient Cre-mediated inversion and expected transgene expression. Transfecting the DIO vector alone confirms the absence of leaky expression. Reporter and Application Format DIO lentiviral vectors are available with GFP, mCherry, YFP, and other fluorescent reporters &mdash; as the primary transgene or fused via a 2A self-cleaving peptide for simultaneous labelling and functional expression. Optogenetic actuators (ChR2, eNpHR, Arch) and chemogenetic tools (DREADDs) are common applications. 
<h2 dir="ltr"> Specifications Context</h2>
 DIO (Double-floxed Inverted Orientation) lentiviral vectors enable Cre-dependent gene expression using recombination-based activation of inverted transgene cassettes. These systems are commonly used in conditional expression workflows involving fluorescent reporters, selectable markers, and controlled gene expression models. A known technical consideration is baseline leakiness before Cre recombination, which can vary depending on vector design and experimental conditions. MBP&rsquo;s DIO lentiviral vector offerings include blank, GFP, and selection-marker formats designed to support a range of Cre-dependent experimental setups. Product availability reflects MBP&rsquo;s catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get a reliable Cre-inducible gene expression (DIO) vector for your lab.

Cre-Inducible Gene Expression (DIO) Vectors

Vector Brochure

AAV–General Guideline to Serotype Selection

AAV-Infection Guideline

Lentivirus-Infection Guideline

Lenti-CMV-DIO-copGFP-SV40-Puro Vector

Unit Quantity

Shipping Condition

<h2 dir="ltr"> What Are Lentiviral Cloning Vectors?</h2>
 The transfer plasmid is the heart of every lentiviral construct &mdash; it defines the transgene cargo, promoter driving expression, selection marker, and structural LTR elements that enable stable genomic integration. Selecting the right cloning vector backbone is the first and most consequential decision in any lentiviral project.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif;">Expression vectors featuring CMV, EF1&alpha;, PGK, and UbC promoter configurations for transgene expression studies</li>
<li>Fluorescent reporter vectors incorporating GFP, RFP, and CFP for cell tracking and visualization</li>
<li>Promoterless vectors for promoter characterization and regulatory element analysis</li>
<li>HA-tag and His-tag expression vectors for protein detection and purification workflows</li>
<li>Bicistronic and tricistronic vector systems for multi-gene expression applications</li>
<li>miRNA- and siRNA-compatible cloning vectors for RNA interference studies</li>
<li>Functional vectors including selectable-marker and HSVtk-based constructs for specialized research applications</li>
</ul>
<h2 dir="ltr"> How to Choose a Lentiviral Cloning Vector</h2>
&nbsp;
Vector Design
Expression vectors, reporter vectors, promoterless systems, tagged constructs, and multicistronic backbones are designed for different experimental objectives. Vector selection should be guided by the intended application, transgene design, and downstream analysis requirements.
Promoter Selection
Promoters influence the strength and pattern of transgene expression. Available vector configurations include CMV, EF1&alpha;, PGK, and UbC promoters, as well as promoterless backbones for studies focused on promoter activity and regulatory elements.
Reporter Requirements
Fluorescent reporters such as GFP, RFP, and CFP enable visualization of transduced cells, monitoring of expression, and assessment of transduction efficiency. Reporter selection should align with the imaging platform and experimental workflow.
Expression Configuration
Single-gene, bicistronic, and tricistronic vector designs provide different levels of flexibility for co-expression studies. Multi-cassette systems can simplify experiments requiring coordinated expression of multiple genes or reporter elements.
Insert Size Considerations
Lentiviral vectors can accommodate relatively large expression cassettes compared with several other viral delivery systems. However, larger inserts may reduce viral production efficiency and should be evaluated during vector design and validation.
<h2 dir="ltr"> Specifications Context</h2>
 Modern research lentiviral vectors are commonly designed with self-inactivating (SIN) long terminal repeats (LTRs), an important biosafety feature that reduces promoter activity within the integrated viral LTR following transduction. Many lentiviral vector systems also incorporate regulatory elements that support efficient transgene expression and vector performance. Vector architecture, promoter selection, reporter configuration, and insert size can all influence downstream cloning, viral production, and expression outcomes. Product availability reflects MBP's catalogue as of mid-2026. 
The search for reliable lentiviral cloning vectors is over. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the perfect match today.

Cloning Vectors

Selection-Drug Killing Curve

QC-LV073 (Lot No. SY7947)

Lenti-CMV-GFP-2A-Puro Vector

Vector

Note

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Lenti-CMV-GFP-2A-Puro-Blank Lentivirus

Vector Map

Titer

Caution

Lenti-CMV-RFP-2A-Puro-Blank Lentivirus

Lenti-EF1a-Blank Lentivirus

Lenti-EF1a-Blank-SV40-Hygro Control Vector

List of Markers

Lenti-EF1a-Blank-SV40-Neo Control Vector

Lenti-EF1a-GFP-2A-Puro Vector

<h2 data-section-id="1vscfio" data-start="222" data-end="262">What are TERT overexpression viruses?</h2>
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TERT overexpression viruses are recombinant lentiviral (or in some cases retroviral) particles carrying the human telomerase reverse transcriptase (hTERT) gene under a constitutive promoter, designed to extend telomere length and bypass replicative senescence in primary cells. Related entities include telomere maintenance, replicative senescence, population doubling limits, EF1-alpha and CMV promoters, and antibiotic/fluorescent selection markers (puromycin, hygromycin, GFP-Puro).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Adeno-hTERT Antisense used in cancer research for inhibition of telomerase activity&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-hTERT Virus (High Titer) used as a standard tool for efficient immortalization of primary cells&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-EF1a-hTERT Virus uses the EF1a promoter for reliable, long-term expression.</li>
<li dir="ltr" role="presentation">Adeno-hTERT is used in transient TERT expression for high-efficiency adenoviral delivery</li>
<li dir="ltr" role="presentation">Lenti-hTERT is used as a streamlined vector for specialized genetic engineering.</li>
<li dir="ltr" role="presentation">Lenti-hTERT-2A-CDK4 Virus co-expression for complex cell types like myogenic cells.</li>
<li dir="ltr" role="presentation">Lenti-EF1a-hTERT-Hygro Virus for precise metabolic selection featuring hygromycin resistance&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-hTERT-Neo Virus for stable generation of cell lines, includes neomycin/G418 resistance&nbsp;</li>
</ul>
&nbsp;
<h2 data-section-id="t86qiz" data-start="932" data-end="983">How to choose TERT overexpression virus products</h2>
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Promoter selection
EF1-alpha promoter constructs are often preferred for primary cells where CMV promoter silencing over extended culture is a concern, though CMV-driven constructs offer strong initial expression; the choice may depend on the expected culture duration and cell type-specific promoter activity.
Selection marker type
Antibiotic resistance markers (puromycin, hygromycin, neomycin/G418, blasticidin) allow selection of stably transduced populations by drug treatment, while fluorescent-antibiotic fusion markers (e.g., GFP-Puro) additionally allow visual confirmation of expression and flow sorting; confirm the target cell line's existing markers don't conflict.
Titer and transduction efficiency
hTERT lentivirus is typically supplied at titers around 1x10^8 TU/mL; transduction efficiency depends on the target cell type's susceptibility to lentiviral infection, and some difficult-to-transduce primary cells may require optimization of multiplicity of infection (MOI) or use of transduction enhancers.
Species considerations
hTERT is human-specific; for non-human primary cells, confirm whether a species-matched TERT ortholog is available or whether SV40 large T antigen (with broader documented cross-species activity) is a more appropriate alternative for the target species.
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<h2 data-section-id="1fljvas" data-start="3151" data-end="3176">Specifications context</h2>
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TERT overexpression lentivirus is typically supplied at approximately 1x10^8 TU/mL in small-volume vials (e.g., 25 microliters per vial), shipped on dry ice and stored at -80&deg;C, with constructs validated by full-length sequencing and confirmed free of mycoplasma and common contaminants. Stable transduction and selection typically take 1-2 weeks before characterization, with full confirmation of telomere extension and extended proliferative capacity assessed over subsequent passages. As of 2026, hTERT lentivirus remains a standard tool for generating immortalized cell lines that retain much of the original primary cell's phenotype, distinguishing it from the more transformation-prone SV40 large T antigen approach.
This sub-category sits within MBP's cell immortalization catalog alongside SV40T antigen overexpression viruses and additional immortalization agents. Labs building custom expression constructs can also review MBP's cloning reagents for vector and competent cell options. MBP's specialist team can help confirm promoter and selection marker choices for a specific primary cell type before order placement.
Browse our collection and <a href="https://mbpinc.net/contact-us">contact the expert team at MBP</a> today to find premium-quality TERT viral vectors for your lab.&nbsp;