<h2 dir="ltr"> What Are Lentiviral Purification &amp; Packaging Kits?</h2>
 Producing high-quality lentiviral stocks is a prerequisite for reproducible transduction experiments. Low-titer or impure virus preparations reduce transduction efficiency, introduce toxicity from residual cell debris, and compromise experimental reproducibility. Purification and packaging kits address every step from plasmid transfection through virus harvest and concentration. Vector production quality depends on efficient purification and packaging workflows. Choose the kit designed for your system.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Lentivirus Bundles combining transduction enhancers, transfection reagents, packaging mix, titer kits for complete workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Packaging System Mix for standardized viral production workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
DNAfectin&trade; Plus and Packaging Mix systems for enhanced transfection workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Lentivirus Promoter Blast&trade; Kit for lentiviral expression studies driven by promoters
</li>
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SARS-CoV-2 S Protein Pseudotype Lentiviral Packaging Mix for pseudovirus-based research applications
</li>
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Ultra-Pure Lentivirus Purification Kit for viral cleanup and concentration
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
ViralEntry&trade; Transduction Enhancer for improved transduction
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
qPCR Lentivirus Titer Kit &amp; ViralEntry&trade; Bundle for optimized transduction workflows and&nbsp; viral quantification&nbsp;
</li>
</ul>
<h2 dir="ltr"> How to Choose a Purification &amp; Packaging Kit</h2>
 Concentration Method Available PEG precipitation kits work with a standard benchtop centrifuge, producing 10&ndash;100-fold concentration &mdash; the most accessible method for labs without ultracentrifuges. Ultrafiltration (100 kDa cutoff) offers high recovery (&gt;80%) while removing small molecules and unincorporated VSV-G. Ultracentrifugation provides the highest concentration (up to 1000&times;) but requires specialised equipment. Packaging Plasmid Generation 3rd generation systems (pMDLg/pRRE + pRSV-Rev + pVSV-G) split packaging elements across three plasmids and eliminate Tat for biosafety. 2nd generation systems use a single packaging plasmid + pVSV-G; some transfer vectors with RSV promoters produce lower titers with 3rd generation packaging unless using CMV-promoter variants (pLKO.1C). Starting Volume Scale Ultrafiltration units are compatible with both small-scale (&le;20 mL) and large-scale (up to 1 L) starting volumes using appropriately sized membranes. Plasmid Transfection Ratio For 3rd generation packaging, a 4:2:1:1 ratio of transfer vector:pMDLg/pRRE:pVSV-G:pRSV-Rev has been validated to maximise titer, with 20 &micro;g transfer vector per 15 cm dish as a standard starting point. Titer Assessment Method p24 ELISA measures total viral particle content; functional transduction assays (GFP or antibiotic selection) measure infectious titer; qPCR-based RNA titration measures packaged RNA copies. Functional titer is most relevant for transduction experiments. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral concentration workflows commonly combine PEG precipitation, ultrafiltration, or density-gradient methods to increase viral titer without requiring ultracentrifugation. Ultrafiltration systems using 100 kDa membranes efficiently retain lentiviral particles (approximately 80&ndash;120 nm) while removing smaller contaminants and free proteins. Following concentration, viral stocks are typically aliquoted into single-use volumes and stored at &minus;80 &deg;C because infectivity decreases with repeated freeze&ndash;thaw cycles and prolonged storage above freezing temperatures. Modern 2nd- and 3rd-generation packaging systems separate essential viral functions across multiple plasmids, including a dedicated VSV-G envelope plasmid, to support safer lentiviral production workflows. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. and get reliable lentiviral purification and packaging kits for your lab today.

Purfication & Packaging Kits

DataSheet

MSDS - LV900

MSDS  - G2500

MSDS - G515

2nd Generation Lentivirus Bundle 2 (LV003-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit, G515-Transduction Enhancer)

Unit Quantity

Applications

Shipping Condition

Lentiviral System FAQ

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Enhanced Lentivirus Safety Features-Replication Incompetency

Suggested MOI for Common Cancer Cell Lines

2nd Generation Packaging System Mix

Note

MSDS - G2500

3rd Gen. Packaging Mix & DNAfectin™ Plus Combo Pack

Additional Information

Storage Condition	

3rd Generation Lentivirus Bundle 3 (LV053-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit)

3rd Generation Lentivirus Bundle 4 (LV053-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit, G515-Transduction Enhancer)

Enhanced Lentivirus Safety Features Replication Incompetency

3rd Generation Packaging System Mix

<h2 dir="ltr">What Are Single-Channel Pipettors?</h2>
 A single-channel pipettor is the single most widely used liquid handling instrument in molecular biology, cell biology, and clinical research. It transfers precise volumes between 0.1 &micro;L and 5,000 &micro;L, one well or tube at a time, using an air-displacement piston mechanism. The piston creates a partial vacuum that aspirates liquid into a disposable tip; advancing the piston fully dispenses the liquid. A spring-loaded tip ejector releases the used tip without hand contact for contamination control. Manual models depend on finger force and offer simple, battery-free operation; electronic models add programmable aspiration/dispensing speeds, multi-dispense modes, and up to 5 custom pipetting programs.&nbsp; A set of four manual single-channel pipettors (P10, P20, P200, P1000) covers 95% of molecular biology liquid handling; add electronic models for high-throughput repetitive work.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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Adjustable volume single-channel pipettors for flexible workflows
</li>
<li dir="ltr" aria-level="1">
Fixed volume pipettors for repeatable dispensing tasks
</li>
<li dir="ltr" aria-level="1">
Mechanical pipettors for standard lab use
</li>
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Ultra-low volume micropipettors for precise &micro;L handling
</li>
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Wide-range pipettors covering &micro;L to mL volumes
</li>
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Autoclavable pipettors for sterile environments
</li>
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Lightweight ergonomic pipettors for long sessions
</li>
</ul>
<h2 dir="ltr"> How to Choose a Single-Channel Pipettor</h2>
 Volume Range: Build a Complete Bench Set A standard four-pipettor bench set covers P10 (1&ndash;10 &micro;L), P20 (2&ndash;20 &micro;L), P200 (20&ndash;200 &micro;L), and P1000 (100&ndash;1,000 &micro;L). For sub-microliter work (sequencing, single-cell), add a P2 (0.2&ndash;2 &micro;L). For large-volume dispensing (media prep, column loading), add a P5000 (1,000&ndash;5,000 &micro;L). Avoid selecting a volume range that places your target dispense below 20% of the maximum &mdash; accuracy degrades at the low end of any range. Manual vs Electronic Manual single-channel pipettors (Eppendorf Research Plus, Gilson PIPETMAN Classic, Rainin Pipet-Lite XLS+) are the standard for routine lab work: field-calibratable, autoclavable, and requiring no power source. Electronic single-channel pipettors (Eppendorf Research Pro, Rainin Pipet-Lite XLS+ Electronic) add: programmable aspiration/dispense speed (5 speeds), multi-dispense (repeat pipetting) mode, mix mode, dilution mode, and backlight LCD volume display. Electronic models reduce hand fatigue in workflows exceeding 200&ndash;300 tip changes per day. Tip Compatibility Three tip systems cover the market: standard conical (universal fit &mdash; fits Eppendorf Research Plus, Gilson PIPETMAN, Rainin non-LTS, Thermo, Sartorius/BioHit); Rainin LTS cylindrical (requires LTS-specific tips &mdash; lower force insertion); Eppendorf epT.I.P.S. proprietary (tightest fit for Eppendorf instruments). Select the tip system that matches your pipettor purchase and existing tip inventory before committing to a brand. Autoclavability and Chemical Resistance Manual pipettors from Eppendorf, Gilson, and Rainin have fully autoclavable lower sections (121&deg;C, 15 psi, 15 min). Electronic pipettors have autoclavable nose cones only &mdash; the electronic module cannot be autoclaved. Calibration and Service All single-channel pipettors are factory-calibrated per ISO 8655. Field recalibration tools are available for Eppendorf, Gilson, and Rainin to perform in-lab gravimetric verification without external service. 
<h2 dir="ltr"> Specifications Context </h2>
 ISO 8655 Part 2 accuracy spec for a 200 &micro;L air-displacement pipettor: &plusmn;0.8 &micro;L systematic error, &le;0.3 &micro;L random error (Eppendorf Research Plus specification at 200 &micro;L). Most manual pipettors weigh 70&ndash;120 g; electronic models weigh 150&ndash;220 g. Operating temperature range: typically 15&ndash;40&deg;C. Eppendorf Research Plus, Gilson PIPETMAN Classic, and Rainin Pipet-Lite XLS+ LTS remain the three dominant single-channel pipettor platforms in North American research labs.&nbsp; 
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Ready to find your new favorite lab tool? Don&rsquo;t just settle for any grip&mdash;find the one that fits your hand and your science. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Reach out to the MBP team</a> today for a customized quote.

Single Channel Pipettors

4 Pipette Bundle Pack (Set of one each of B250, B251, B252 and B253)

Volume

Shipping condition

Microcentrifuge Accessories

<h2>What Are Microcentrifuge Accessories?</h2>
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Microcentrifuge accessories maintain performance, extend tube-format versatility, and ensure safe operation of benchtop microcentrifuges. These include replacement fixed-angle rotors for 12&ndash;24 &times; 1.5/2.0 mL tube positions, tube adapters for 0.5 mL and 0.2 mL formats, aerosol-tight sealed rotor lids for biocontainment of infectious specimens, balance tubes for partial-load runs, and specialty rotors for PCR strips or 5.0 mL tubes where supported by the centrifuge model. All microcentrifuge accessories are model-specific, as rotor coupling systems and tube bore dimensions differ between manufacturers and even between models within the same brand, making exact model verification essential before ordering.
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<h2>What You Will Find:</h2>
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<ul>
<li>High-quality adapters and rotors for precise and reliable tube fit</li>
<li>Multiple formats including PCR strips, microtubes, plates, and spin columns</li>
<li>Specialized and high-capacity rotors for enhanced compatibility</li>
<li>Swing-out and sealed options for reliable and contamination-free processing</li>
</ul>
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<h2>How to Choose Microcentrifuge Accessories</h2>
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Replacement Rotor Identification
The rotor model number is typically printed on the rotor body or listed in the centrifuge user manual. Cross-reference it with the manufacturer's accessory catalog to confirm that the coupling system matches the centrifuge motor shaft. For standard 12-position rotors, verify compatibility with both 1.5 mL and 2.0 mL microtubes.
Tube Adapter Sizing
For 0.5 mL adapters, ensure the outer diameter closely matches the rotor bore so the tube remains secure during operation. For 0.2 mL adapters, confirm that PCR tubes are positioned correctly to achieve the intended centrifugal force while maintaining stability throughout the run.
Aerosol-Tight Sealed Lids
Choose sealed lids rated for the rotor's maximum operating RCF. O-rings should be inspected regularly for flattening, cracking, or discoloration and replaced immediately if any signs of wear appear. Maintaining replacement O-rings for frequently used lids helps ensure continued safe operation.
Specialty Rotors
Some microcentrifuge models support optional rotors for 5.0 mL tubes, PCR strips, or microplates. These accessories expand workflow flexibility but may operate at lower maximum RCF values than standard 1.5 mL rotors. Always verify the rated speed and force limits before use.
Balance Tubes
Balance tubes help maintain proper rotor balance during partial-load runs. They should closely match the mass and dimensions of the sample tubes being processed. Proper balancing reduces vibration, minimizes bearing wear, and supports long-term centrifuge performance.
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<h2>Specifications Context</h2>
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Many modern fixed-angle rotors and polypropylene adapters are autoclavable, although specific sterilization requirements vary by manufacturer and material. Always follow the OEM maintenance and decontamination guidelines for each accessory. Rotor service life is generally determined by cumulative operating hours and manufacturer recommendations rather than visual appearance alone.
Sealed rotor lid systems with one-touch release mechanisms have become standard in modern molecular diagnostic laboratories, providing improved safety, easier maintenance, and enhanced biocontainment for regulated workflows.
Stop wasting time searching. The best microcentrifuge accessories are right here at MBP Products. <a href="https://mbpinc.net/contact-us">Connect with our expert team</a> to get your equipment accessories today.

50 ml Swing Rotor Buckets

The Q5122 by Applied Biological Materials (ABM) is a replacement set of 2 swing rotor buckets for the ABM Q5120 Benchtop Centrifuge (4,000 rpm / 2,720 × g). Each bucket holds 2 × 50 ml conical tubes (4 total per set). Keeps a spare set on hand to maximize Q5120 throughput or replace worn components.

Manufacture

<h2 class="p2"> What are Glass Bottom Cell Culture Plates?</h2>
 Glass bottom cell culture plates are sterile, single-use multi-well plates combining a polystyrene plate body with a borosilicate coverglass floor in each well, designed for high-resolution live-cell microscopy applications that require the optical clarity and low autofluorescence of glass rather than polystyrene. Glass bottom plates are required for confocal microscopy, TIRF microscopy, spinning disk confocal, super-resolution (STORM, PALM, STED), and any high-magnification live-cell imaging protocol that specifies No. 1.5 coverglass.
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<h2 class="p1">What you will find:</h2>
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&bull; High clarity glass bottoms for crisp, high resolution imaging &bull; Compatible with fluorescence, confocal, and phase contrast microscopy &bull; Treated surfaces to support strong cell attachment &bull; Multi-well formats for parallel experiments and higher throughput &bull; Sterile, ready-to-use plates to keep your workflow clean &bull; Durable construction to handle repeated imaging sessions
<h2 class="p2"> How to Choose Glass Bottom Cell Culture Plates</h2>
 Why Glass Bottom vs. Standard Polystyrene Plates Standard polystyrene cell culture plates have optical properties inadequate for high-resolution microscopy: polystyrene autofluoresces in the 400&ndash;600 nm range, interfering with DAPI, GFP, and Cy3 channels; the relatively thick PS well bottom (0.5&ndash;2.0 mm vs. 0.16&ndash;0.19 mm for No. 1.5 coverglass) places cells outside the working distance of high-NA oil objectives; and PS birefringence creates artefacts in polarized light and DIC microscopy. Borosilicate glass (No. 1.5, 0.16&ndash;0.19 mm) is optically isotropic, produces no autofluorescence in the visible range, and matches the focal correction of oil-immersion objectives. Coverglass Thickness: No. 1.5 Standard No. 1.5 coverglass (0.16&ndash;0.19 mm nominal, ~0.17 mm mean) is the standard for which virtually all high-NA microscope objectives (Nikon Plan Apo, Olympus UPLSAPO, Zeiss Plan-Apochromat) are spherical aberration&ndash;corrected. NEST Scientific glass bottom plates use 0.16&ndash;0.19 mm borosilicate glass per product specification. For objectives with correction collars, the correction collar should be set to match the actual glass thickness measured per lot. Medical-Grade Adhesive and Non-Cytotoxicity The borosilicate glass disk in each well is bonded to the PS plate body using medical-grade adhesive confirmed non-cytotoxic by NEST Scientific lot testing. The adhesive is the most critical quality checkpoint for glass bottom plates: cytotoxic adhesive leaches compounds that affect cell morphology, viability, and signaling within 24&ndash;48 hours of seeding. Always confirm non-cytotoxicity certification on the manufacturer's lot data sheet before use with sensitive primary cells. 6-Well vs. 24-Well Format NEST Scientific 6-well glass bottom plates (glass diameter 20 mm per well) are used for experiments requiring larger cell populations visible in a single field at low magnification (10&times;&ndash;20&times;) &mdash; migration assays, wound-healing scratch assays, live-cell time-lapse at moderate magnification, and spheroid imaging. 24-well glass bottom plates (glass diameter 13.5 mm per well) support medium-throughput confocal imaging with smaller cell populations per well &mdash; transfection imaging, localization studies, co-localization panels, and FISH on adherent cells. TC-Treated Glass Bottom NEST Scientific glass bottom plates have both the polystyrene well walls and the glass floor TC-treated, ensuring that adherent mammalian cells attach and grow directly on the glass surface. Without TC treatment of the glass floor, adherent cells form uneven, loosely attached monolayers that detach during media changes before imaging. Immersion Objective Compatibility For oil-immersion objectives (working distance &lt;0.2 mm at 100&times;), glass bottom plates require the inverted microscope objective to be positioned below the plate base &mdash; the standard configuration for inverted microscopes (Nikon Ti2, Olympus IX83, Zeiss Axio Observer). Confirm that your microscope stage adapter accepts standard multi-well plate footprints and that the plate bottom is accessible to the objective lens. 
<h2 class="p2">&nbsp; Specifications Context</h2>
 NEST Scientific glass-bottom cell culture plates use 100% virgin polystyrene plate bodies and borosilicate glass inserts (0.16&ndash;0.19 mm), bonded with medical-grade, non-cytotoxic adhesive. Both the polystyrene well wall and the glass floor are TC-treated by vacuum plasma. E-beam sterilized to SAL 10⁻⁶; certified DNase/RNase-free, non-pyrogenic per lot. Compatible with confocal microscopy, high-resolution microscopy, DIC, polarized light, phase contrast, TIRF, and live-cell fluorescence imaging. Typical applications include: STEM cell research, cell cycle regulation, GFP localization, laser scanning confocal, two-photon confocal, and infrared imaging per NEST Scientific product documentation. NEST Scientific glass-bottom plates in 6-well and 24-well formats are stocked by MBP. 
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Upgrade your imaging setup with plates built for clarity and consistency. <a href="https://mbpinc.net/contact-us">Contact MBP</a> to get your Glass Bottom Cell Culture Plates today!

Glass Bottom Cell Culture Plates

6-Well Multiwell Cell Plates

Recommended Medium Volume

Cell Growth Area

Sterility

<h2 dir="ltr">What Are AAV Control &amp; Reporter Constructs?</h2>
 Establishing reliable experimental controls is critical in AAV research because transduction efficiency varies substantially by serotype, cell type, multiplicity of infection (MOI), and batch-to-batch variation in viral preparation quality. Matched AAV control and reporter constructs provide the reference point for interpreting experimental results.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Promoter-Specific Blank Vectors used for baseline control, utilize neuron-specific hSyn promoters and high-purity plasmids&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Serotype-Matched Blank Viruses for precise tissue-targeting validation. Available pre-packaged in serotypes 1 through 11
</li>
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Bioluminescent Reporter Systems for sensitive measurement of viral delivery.&nbsp; And gene expression featuring luciferase and SV40-luc markers
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Fluorescent Control Constructs carry circ-GFP, GFP, and CBh-gcGFP, for real-time transduction tracking.
</li>
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Circular RNA Control Plasmids for advanced non-coding RNA research.&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Cloning-Ready Expression Backbones for selected gene insertion and viral packaging workflows using ampicillin-resistant blank vectors
</li>
</ul>
<h2 dir="ltr"> How to Choose AAV Control &amp; Reporter Constructs </h2>
 Serotype and Backbone Matching The control construct should use the same AAV serotype, ITR configuration, and promoter as the experimental construct, ensuring transduction efficiency differences reflect the transgene rather than vector or serotype variation. Fluorescent Reporter Choice GFP and mCherry reporters allow confirmation of transduction by fluorescence microscopy or flow cytometry, with mCherry offering a red-channel option for multiplexed experiments. Luciferase and Dual-Reporter Options Firefly luciferase provides quantitative bioluminescent transduction confirmation; dual reporter systems (e.g., fluorescent + luciferase) allow both visual and quantitative readouts from the same construct. Empty Vector Baseline An empty AAV vector &mdash; same ITRs, serotype, and promoter, no transgene &mdash; establishes the baseline effect of AAV capsid delivery and innate immune activation, independent of the gene of interest. Batch-to-Batch Variation Controls Because AAV preparation quality can vary between batches, including a transduction-efficiency control (e.g., GFP reporter) alongside each new batch of experimental AAV helps confirm comparable functional titer. 
<h2 dir="ltr"> Specifications Context</h2>
 AAV transduction can activate innate immune sensing pathways and, at high vector doses, induce cellular stress responses independently of the transgene. A matched empty vector or reporter control sharing the same serotype and promoter as the experimental construct helps distinguish vector-related effects from genuine transgene-specific phenotypes. Because AAV infectivity can vary between preparations, reporter-based transduction assessments are commonly used to verify functional performance before experimental studies. For in vivo applications, pilot reporter studies can help confirm tissue targeting and delivery efficiency before larger cohorts are committed. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to get high-quality control/reporter vectors for your lab.