ABM Reagents

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Lenti-UbC-Blank-CBh-RFP-2A-Puro Control Vector

Lenti-UbC-Blank-CBh-RFP-2A-Puro Control Vector Blank0623 (ABM) — 1.0 µg. Blank UbC lentiviral control with CBh-RFP-2A-Puro. Red fluorescence for transduction monitoring + puromycin selection. Use when experimental construct uses GFP to avoid spectral overlap. Store −20 °C. RUO.

Unit Quantity

List of Markers

Shipping Condition

Lenti-UbC-Blank-SV40-Blast Control Vector

Lenti-UbC-Blank-SV40-Blast Control Vector Blank0621 (ABM) — 1.0 µg. Blank UbC promoter lentiviral negative control with SV40-Blasticidin selection. Orthogonal to Hygro (Blank0620) and Neo (Blank0619) for multi-marker experiments. Store −20 °C. RUO.

Lenti-UbC-Blank-SV40-Hygro Control Vector

Lenti-UbC-Blank-SV40-Hygro Control Vector Blank0620 (ABM) — 1.0 µg. Blank UbC promoter lentiviral negative control with SV40-Hygro selection marker. For controlling UbC-driven lentiviral experiments; hygromycin B selection confirms stable transduction. Store −20 °C. RUO.

Lenti-UbC-Blank-SV40-Neo Control Vector

Lenti-UbC-Blank-SV40-Neo Control Vector Blank0619 (ABM) — 1.0 µg. Blank UbC promoter lentiviral negative control with SV40-Neo (G418/neomycin resistance). For UbC-driven lentiviral experiment negative controls with neomycin selection. Store −20 °C. RUO.

<h2 dir="ltr">What Are miRNA Lentiviral Negative Controls?</h2>
 MicroRNAs (miRNAs) are approximately 22-nucleotide non-coding RNAs that regulate gene expression post-transcriptionally by binding to the 3&prime; UTR of target mRNAs, triggering mRNA degradation or translational repression via the RNA-induced silencing complex (RISC). Lentiviral delivery of miRNA expression constructs enables stable, long-term modulation of miRNA activity &mdash; essential where transient transfection approaches are insufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Control Viruses models used in miRNA overexpression studies, for negative control normalization
</li>
<li dir="ltr" aria-level="1">
Validation of 3'UTR target utilizing bioluminescent-based reporter assays.
</li>
<li dir="ltr" aria-level="1">
miRNA-Off Control Lentiviruses used to baseline microRNA knockdown effects.
</li>
<li dir="ltr" aria-level="1">
Blank 5&rsquo; UTR reporter vector optimized for gene expression normalization and translational regulation studies&nbsp;
</li>
<li dir="ltr" aria-level="1">
Reporter Stable Cell Lines models with Luciferase or GFP markers for reliable assay standardization.
</li>
</ul>
<h2 dir="ltr"> How to Choose a miRNA Negative Control</h2>
 Experiment Type Matching miRNA mimic (gain-of-function) experiments require a non-targeting miRNA mimic control. miRNA inhibitor/antagomiR (loss-of-function) experiments require a non-targeting inhibitor control. Lentiviral miRNA expression requires an empty vector or scrambled miRNA sequence. Mimic Control Design A non-targeting miRNA mimic control is a double-stranded RNA molecule resembling a mature miRNA duplex but with no significant homology to any known miRNA target in human, mouse, or rat transcriptomes &mdash; entering RISC the same way as a functional mimic. Inhibitor Control Design Non-targeting miRNA inhibitor (antagomiR) controls are single-stranded antisense sequences that enter the relevant cellular compartment without blocking any known miRNA, establishing the baseline for inhibitor-treated versus non-inhibitor comparisons. Vector Backbone for Lentiviral Delivery Stable lentiviral expression of miRNAs is used for long-term gain-of-function studies; the matched non-targeting lentiviral control must use the same promoter and vector backbone as the experimental construct to isolate sequence-specific effects. Inducible Format Availability Tet-On (doxycycline-responsive) non-targeting miRNA lentiviral controls are available for experiments requiring temporal control, used alongside inducible experimental constructs to control for doxycycline treatment itself. 
<h2 dir="ltr"> Specifications Context</h2>
 miRNA controls are designed to reflect the endogenous processing pathway of microRNAs, including Drosha and Dicer-mediated maturation before RISC loading. Because siRNA and miRNA constructs enter the RNA interference pathway differently, pathway-matched controls are important for accurate experimental interpretation. Non-targeting miRNA controls help distinguish sequence-specific effects from background effects associated with vector delivery and miRNA pathway activation. Available formats include lentiviral vectors, packaged viruses, reporter constructs, and stable cell line controls for miRNA and UTR-based studies. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and request a quote for efficient miRNA control requirements.

miRNA Control

Lenti-UTR-GFP-Blank Virus

m015 by Applied Biological Materials is a pre-packaged Lenti-UTR-GFP-Blank control virus with no 3′ UTR insert. Establishes the no-3′UTR baseline GFP signal for lentiviral 3′ UTR miRNA reporter experiments. Ready to transduce. Store at −80°C; shipped on dry ice.

Vector Map

Titer

Storage Condition

Lenti-UTR-Luc-Blank Virus

m013 by Applied Biological Materials is a pre-packaged lentiviral firefly luciferase control virus produced from the pLenti-UTR-Luc-Blank (m012) backbone with an empty MCS — no 3'UTR insert. Supplied as 4 × 500 µl aliquots; no packaging required. Use as a matched transduction baseline control for m012-based miRNA 3'UTR reporter assays. Store at &minus;80°C; do not refreeze thawed aliquots.

<h2 dir="ltr"> What Are Lentiviral Purification &amp; Packaging Kits?</h2>
 Producing high-quality lentiviral stocks is a prerequisite for reproducible transduction experiments. Low-titer or impure virus preparations reduce transduction efficiency, introduce toxicity from residual cell debris, and compromise experimental reproducibility. Purification and packaging kits address every step from plasmid transfection through virus harvest and concentration. Vector production quality depends on efficient purification and packaging workflows. Choose the kit designed for your system.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Lentivirus Bundles combining transduction enhancers, transfection reagents, packaging mix, titer kits for complete workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Packaging System Mix for standardized viral production workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
DNAfectin&trade; Plus and Packaging Mix systems for enhanced transfection workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Lentivirus Promoter Blast&trade; Kit for lentiviral expression studies driven by promoters
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
SARS-CoV-2 S Protein Pseudotype Lentiviral Packaging Mix for pseudovirus-based research applications
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Ultra-Pure Lentivirus Purification Kit for viral cleanup and concentration
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
ViralEntry&trade; Transduction Enhancer for improved transduction
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
qPCR Lentivirus Titer Kit &amp; ViralEntry&trade; Bundle for optimized transduction workflows and&nbsp; viral quantification&nbsp;
</li>
</ul>
<h2 dir="ltr"> How to Choose a Purification &amp; Packaging Kit</h2>
 Concentration Method Available PEG precipitation kits work with a standard benchtop centrifuge, producing 10&ndash;100-fold concentration &mdash; the most accessible method for labs without ultracentrifuges. Ultrafiltration (100 kDa cutoff) offers high recovery (&gt;80%) while removing small molecules and unincorporated VSV-G. Ultracentrifugation provides the highest concentration (up to 1000&times;) but requires specialised equipment. Packaging Plasmid Generation 3rd generation systems (pMDLg/pRRE + pRSV-Rev + pVSV-G) split packaging elements across three plasmids and eliminate Tat for biosafety. 2nd generation systems use a single packaging plasmid + pVSV-G; some transfer vectors with RSV promoters produce lower titers with 3rd generation packaging unless using CMV-promoter variants (pLKO.1C). Starting Volume Scale Ultrafiltration units are compatible with both small-scale (&le;20 mL) and large-scale (up to 1 L) starting volumes using appropriately sized membranes. Plasmid Transfection Ratio For 3rd generation packaging, a 4:2:1:1 ratio of transfer vector:pMDLg/pRRE:pVSV-G:pRSV-Rev has been validated to maximise titer, with 20 &micro;g transfer vector per 15 cm dish as a standard starting point. Titer Assessment Method p24 ELISA measures total viral particle content; functional transduction assays (GFP or antibiotic selection) measure infectious titer; qPCR-based RNA titration measures packaged RNA copies. Functional titer is most relevant for transduction experiments. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral concentration workflows commonly combine PEG precipitation, ultrafiltration, or density-gradient methods to increase viral titer without requiring ultracentrifugation. Ultrafiltration systems using 100 kDa membranes efficiently retain lentiviral particles (approximately 80&ndash;120 nm) while removing smaller contaminants and free proteins. Following concentration, viral stocks are typically aliquoted into single-use volumes and stored at &minus;80 &deg;C because infectivity decreases with repeated freeze&ndash;thaw cycles and prolonged storage above freezing temperatures. Modern 2nd- and 3rd-generation packaging systems separate essential viral functions across multiple plasmids, including a dedicated VSV-G envelope plasmid, to support safer lentiviral production workflows. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. and get reliable lentiviral purification and packaging kits for your lab today.

Purfication & Packaging Kits

Enhanced Lentivirus Safety Features-Replication Incompetency

Suggested MOI for Common Cancer Cell Lines

Lentiviral System FAQ

Lentivirus Promoter Blast™ Kit

LV950 by Applied Biological Materials is the Lentivirus Promoter Blast™ Kit: 4 × 1 mL at &gt;1×106 IU/mL GFP lentiviruses comparing CMV (LV008), PGK (LV028), EF1α (LV046), and UbC (LV027) promoters. All with Puromycin selection. For promoter selection and MOI optimization before stable cell line generation. Store at −80°C; shipped on dry ice.

Note

<h2 dir="ltr"> What Are Lentiviral Cloning Vectors?</h2>
 The transfer plasmid is the heart of every lentiviral construct &mdash; it defines the transgene cargo, promoter driving expression, selection marker, and structural LTR elements that enable stable genomic integration. Selecting the right cloning vector backbone is the first and most consequential decision in any lentiviral project.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif;">Expression vectors featuring CMV, EF1&alpha;, PGK, and UbC promoter configurations for transgene expression studies</li>
<li>Fluorescent reporter vectors incorporating GFP, RFP, and CFP for cell tracking and visualization</li>
<li>Promoterless vectors for promoter characterization and regulatory element analysis</li>
<li>HA-tag and His-tag expression vectors for protein detection and purification workflows</li>
<li>Bicistronic and tricistronic vector systems for multi-gene expression applications</li>
<li>miRNA- and siRNA-compatible cloning vectors for RNA interference studies</li>
<li>Functional vectors including selectable-marker and HSVtk-based constructs for specialized research applications</li>
</ul>
<h2 dir="ltr"> How to Choose a Lentiviral Cloning Vector</h2>
&nbsp;
Vector Design
Expression vectors, reporter vectors, promoterless systems, tagged constructs, and multicistronic backbones are designed for different experimental objectives. Vector selection should be guided by the intended application, transgene design, and downstream analysis requirements.
Promoter Selection
Promoters influence the strength and pattern of transgene expression. Available vector configurations include CMV, EF1&alpha;, PGK, and UbC promoters, as well as promoterless backbones for studies focused on promoter activity and regulatory elements.
Reporter Requirements
Fluorescent reporters such as GFP, RFP, and CFP enable visualization of transduced cells, monitoring of expression, and assessment of transduction efficiency. Reporter selection should align with the imaging platform and experimental workflow.
Expression Configuration
Single-gene, bicistronic, and tricistronic vector designs provide different levels of flexibility for co-expression studies. Multi-cassette systems can simplify experiments requiring coordinated expression of multiple genes or reporter elements.
Insert Size Considerations
Lentiviral vectors can accommodate relatively large expression cassettes compared with several other viral delivery systems. However, larger inserts may reduce viral production efficiency and should be evaluated during vector design and validation.
<h2 dir="ltr"> Specifications Context</h2>
 Modern research lentiviral vectors are commonly designed with self-inactivating (SIN) long terminal repeats (LTRs), an important biosafety feature that reduces promoter activity within the integrated viral LTR following transduction. Many lentiviral vector systems also incorporate regulatory elements that support efficient transgene expression and vector performance. Vector architecture, promoter selection, reporter configuration, and insert size can all influence downstream cloning, viral production, and expression outcomes. Product availability reflects MBP's catalogue as of mid-2026. 
The search for reliable lentiviral cloning vectors is over. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the perfect match today.

Cloning Vectors

Lenti-β-Gal Vector

LV011-b by Applied Biological Materials is a third-generation lentiviral transfer vector (10 µg) expressing beta-galactosidase (&beta;-gal) for colorimetric X-gal transduction efficiency assays and stable &beta;-gal reporter cell line generation in mammalian cells. Orthogonal to GFP for dual-label experiments. Shipped on Ice Pack; store at &minus;20°C.

<h2>What are L-glutamine and amino acid supplements?</h2>
&nbsp;
L-glutamine and amino acid supplements are cell culture additives used to support cellular metabolism, growth, viability, and biosynthesis. They provide nutrients that may become depleted during culture or are required in greater quantities than those supplied by basal media alone. These supplements are commonly added to media such as DMEM, RPMI 1640, MEM, and DMEM/F-12 to optimize cell growth and maintain healthy cultures.
L-glutamine is one of the most important supplements in mammalian cell culture because it serves as a major energy source and is required for protein synthesis, nucleotide production, and glutathione synthesis. However, because free L-glutamine is unstable in solution and gradually breaks down into ammonia and pyroglutamate, many laboratories use stabilized alternatives such as GlutaMAX (L-alanyl-L-glutamine) for extended culture applications.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>L-Glutamine stable formulations for sourcing of essential nitrogen</li>
<li>Non-Essential Amino Acids (NEAA) for growth media enhancement</li>
<li>Synthetic Polymer Coatings Poly-L-Ornithine and Poly-L-Lysine solutions for better cell attachment.</li>
<li>Extracellular Matrix Proteins used for cell adhesion and include high-purity Fibronectin and Vitronectin coating reagents</li>
<li>Adhesion Promotion Reagents for improving the surface properties of culture vessels.</li>
</ul>
&nbsp;
<h2>How to choose L-glutamine and amino acid supplements</h2>
&nbsp;
L-glutamine concentration and format
Most mammalian cell culture systems use L-glutamine concentrations between 2&ndash;6 mM, although requirements vary depending on the medium and cell type. It is commonly supplied as:
<ul>
<li>USP-grade powder for custom media preparation</li>
<li>200 mM liquid stock solutions for direct supplementation</li>
</ul>
Liquid stocks should be aliquoted and stored frozen to minimize repeated freeze-thaw cycles and preserve stability.
Understanding glutamine degradation
Free L-glutamine gradually breaks down in aqueous solution through a non-enzymatic process that produces ammonia and pyroglutamate. This degradation accelerates with increasing temperature, storage time, and exposure to common cell culture buffer systems.
Ammonia accumulation can negatively affect cell growth, viability, productivity, and experimental reproducibility, particularly during long culture periods without media replacement.
GlutaMAX and stable glutamine alternatives
GlutaMAX (L-alanyl-L-glutamine) is a stabilized dipeptide form of glutamine designed to resist degradation in culture media. Once taken up by cells, it is converted into usable glutamine while maintaining greater stability during incubation.
<ul>
<li>Reduced ammonia accumulation</li>
<li>Improved media stability</li>
<li>Better performance in long-term cultures</li>
<li>Less frequent media replacement requirements</li>
</ul>
For most applications, GlutaMAX can directly replace standard L-glutamine without protocol modifications.
MEM non-essential amino acids (NEAA)
NEAA supplements provide amino acids that cells can synthesize themselves but may benefit from receiving directly from the culture medium. Supplementation reduces the metabolic burden of amino acid production and often improves growth rates and cell viability.
NEAA solutions are typically supplied as 100X concentrates and diluted to 1X working concentration.
HT supplement
HT supplement contains hypoxanthine and thymidine and is commonly used following HAT selection during hybridoma production. It supports nucleotide synthesis through salvage pathways while cells recover from selection pressure.
MEM vitamin solution
Vitamin supplements provide additional growth-supporting micronutrients that may become limiting during prolonged culture or in demanding applications. These supplements are often used alongside amino acid supplementation to enhance culture performance.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The standard L-glutamine concentration used in many mammalian culture systems is approximately 2 mM, though actual requirements vary by medium formulation and cell type. Free L-glutamine remains stable as a dry powder and frozen stock solution but degrades progressively once added to liquid media.
Stabilized glutamine products such as GlutaMAX have become increasingly common because they maintain glutamine availability while minimizing ammonia accumulation during incubation. These formulations are particularly valuable in long-duration experiments and high-density culture systems.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get research-grade supplements for your lab.

L-Glutamine & Amino Acids

L-Glutamine Solution (200 mM)

G275 by Applied Biological Materials (ABM) is a 200 mM L-Glutamine solution (29.2 g/L) in normal saline, 0.2 µm sterile-filtered. Supplied in 100 mL. Passes sterility testing. Store at −20°C; use within 2 weeks of thawing. For use as a cell culture media supplement.

Usage

Sterility

<h2>What are Bioluminescence Assays?</h2>
&nbsp;
Bioluminescence assays use luciferase enzymes to convert specific substrates into light, producing a highly sensitive readout for gene expression studies or cell viability measurements. In ATP-based systems, light output correlates with cellular ATP levels and therefore cell viability.
Firefly luciferase uses luciferin along with ATP, magnesium, and oxygen to generate a green/yellow signal with a decay time of roughly 12 minutes. Renilla luciferase uses coelenterazine and oxygen, producing a faster-decaying blue signal and not requiring ATP.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Luciferase Assay Systems: High-performance kits like the Luciferase Assay Kit (G287), designed for rapid, flash-type bioluminescence detection that provides a direct and sensitive measure of gene induction.
</li>
<li dir="ltr" aria-level="1">
Fluorescent Control Proteins: Premium GFP Purified Protein (000033P) available as a reliable internal standard or calibration marker to validate transfection efficiency and protein localization across diverse imaging platforms.
</li>
</ul>
&nbsp;
<h2>How to Choose a Bioluminescence Assay System</h2>
&nbsp;
Decide Between Single and Dual-Reporter Designs
Dual-reporter systems combine a test luciferase with a constitutively expressed control luciferase, allowing normalization for variability in transfection efficiency, cell number, and viability.
Choose NanoLuc for Maximum Sensitivity
NanoLuc is an engineered 19 kDa luciferase that produces approximately 100&ndash;150&times; brighter signal than firefly or Renilla systems and uses furimazine as its substrate.
Match Substrate and Cofactor Requirements
Firefly luciferase requires luciferin, ATP, Mg2+, and oxygen, while Renilla luciferase requires coelenterazine and oxygen only, which is important when ATP levels are part of the experimental variable.
Consider Destabilized Reporters for Faster Response
Destabilized luciferase variants have shorter intracellular half-lives, allowing more rapid detection of transcriptional changes with reduced signal lag.
Use ATP-Based Assays for Viability
ATP-based bioluminescence assays measure cellular viability rather than gene expression, as light output reflects ATP levels in metabolically active cells.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Dual-Luciferase Reporter system combines firefly and Renilla luciferase to allow sequential measurement using a luciferase inhibitor for normalization of experimental variability.
As of 2026, NanoLuc-based systems are increasingly used beyond traditional reporter gene assays, including protein interaction and target engagement studies due to their high brightness and low background.
Enhance your signal detection&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional bioluminescence solutions.

Bioluminescence Assays and Reagents

Luc Gene Test with G287 Report-2

Luciferase Assay Kit

Luciferase Assay Kit (G287) by ABM delivers 500 reactions for firefly luciferase reporter gene quantification in mammalian and bacterial cell cultures, with a two-reagent workflow and luminescence readout within 30 minutes.

<h2 dir="ltr">What Are Luciferase Reporter Lentiviral Vectors?</h2>
 Luciferase reporters have been foundational to gene expression research since their first use as reporter genes in 1985. The bioluminescent signal produced when luciferase oxidises its substrate provides a sensitive, quantitative, non-radioactive readout measurable over a wide dynamic range with standard plate reader luminometers. Lentiviral delivery produces stable, integrated reporter cell lines that maintain consistent signal over weeks to months. Accurate reporter assays depend on appropriate detection systems. Select a luciferase construct that fits your research needs.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Luciferase Reporters: Reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background controls with <a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">luciferase reporter systems</a>.</li>
<li>Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li>Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li>Promoter &amp; UTR Reporter Systems: Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li>Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose a Luciferase Reporter</h2>
 Reporter Role Firefly luciferase is typically driven by the promoter or response element under investigation as the experimental reporter; Renilla luciferase &mdash; driven by a constitutive promoter (SV40 or CMV) &mdash; provides within-sample normalisation correcting for cell number, transfection efficiency, and lysis variability. Substrate and Emission Firefly luciferase uses D-luciferin and emits at ~550&ndash;570 nm (green/yellow); Renilla uses coelenterazine and emits at ~480 nm (blue-green) &mdash; both can be measured sequentially from the same lysate in a dual-luciferase assay. Stable vs. Transient Use Lentiviral delivery produces stable reporter cell lines in 1&ndash;2 weeks via antibiotic selection &mdash; essential for high-throughput compound screening, long-term pathway activity monitoring, and in vivo bioluminescence imaging. Pathway-Specific Reporters Promoter or transcriptional response element (TRE) inserts &mdash; such as Wnt/TCF-LEF, NF-&kappa;B, NFAT, or VEGF promoter &mdash; provide a quantitative readout of pathway activity when the cognate transcription factor is activated. In Vivo Imaging Needs CMV-GFP-T2A-Luciferase dual-reporter lentivectors co-express GFP and luciferase for combined fluorescence and bioluminescence tracking of implanted cells in live animals. 
&nbsp;
<h2 dir="ltr">Specifications Context</h2>
 Cells are lysed and mixed with the appropriate substrate in a plate reader or tube luminometer; light emission is measured in relative light units (RLU), proportional to luciferase activity and reflecting promoter activity or transgene expression level. A promoter-less dual luciferase vector establishes the minimum signal level for the assay system, identifying the true zero-activity baseline. Stable lentiviral luciferase reporter cell lines are essential tools for tracking xenograft tumour growth, metastasis, and therapy response in preclinical mouse models. EF1&alpha;-driven constructs maintain high expression for 30+ days without selection, while CMV-driven constructs may undergo progressive silencing in some cell types. Product availability reflects MBP's catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get optimized luciferase reporters for advanced cloning and CRISPR-related workflows.

Luciferase Reporter

LV010086 Vector Map and Sequence

Luciferase (Luciola italica) Lentiviral Vector (CMV)(IRES-GFP)

LV010086 by Applied Biological Materials is a 1.0 µg lentiviral vector carrying Luciola italica red-shifted luciferase (∼612 nm, ABA03040) under a CMV promoter with IRES-GFP co-expression. Improved tissue penetration for in vivo BLI. D-luciferin substrate. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.

Accession Number

Species

LV010137 Sequence

Luciferase (Luciola italica) Lentiviral Vector (minCMV)(Bicistronic)

LV010137 by Applied Biological Materials is a 1.0 µg bicistronic lentiviral vector with Luciola italica red-shifted luciferase (∼612 nm, ABA03040) under minCMV and a PGK-driven MCS in opposing orientation. For in vivo red-shifted BLI and dual-expression studies. D-luciferin substrate. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.