ABM Reagents

<h2 dir="ltr"> What Are Lentiviral Cloning Vectors?</h2>
 The transfer plasmid is the heart of every lentiviral construct &mdash; it defines the transgene cargo, promoter driving expression, selection marker, and structural LTR elements that enable stable genomic integration. Selecting the right cloning vector backbone is the first and most consequential decision in any lentiviral project.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li style="font-family: arial, helvetica, sans-serif;">Expression vectors featuring CMV, EF1&alpha;, PGK, and UbC promoter configurations for transgene expression studies</li>
<li>Fluorescent reporter vectors incorporating GFP, RFP, and CFP for cell tracking and visualization</li>
<li>Promoterless vectors for promoter characterization and regulatory element analysis</li>
<li>HA-tag and His-tag expression vectors for protein detection and purification workflows</li>
<li>Bicistronic and tricistronic vector systems for multi-gene expression applications</li>
<li>miRNA- and siRNA-compatible cloning vectors for RNA interference studies</li>
<li>Functional vectors including selectable-marker and HSVtk-based constructs for specialized research applications</li>
</ul>
<h2 dir="ltr"> How to Choose a Lentiviral Cloning Vector</h2>
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Vector Design
Expression vectors, reporter vectors, promoterless systems, tagged constructs, and multicistronic backbones are designed for different experimental objectives. Vector selection should be guided by the intended application, transgene design, and downstream analysis requirements.
Promoter Selection
Promoters influence the strength and pattern of transgene expression. Available vector configurations include CMV, EF1&alpha;, PGK, and UbC promoters, as well as promoterless backbones for studies focused on promoter activity and regulatory elements.
Reporter Requirements
Fluorescent reporters such as GFP, RFP, and CFP enable visualization of transduced cells, monitoring of expression, and assessment of transduction efficiency. Reporter selection should align with the imaging platform and experimental workflow.
Expression Configuration
Single-gene, bicistronic, and tricistronic vector designs provide different levels of flexibility for co-expression studies. Multi-cassette systems can simplify experiments requiring coordinated expression of multiple genes or reporter elements.
Insert Size Considerations
Lentiviral vectors can accommodate relatively large expression cassettes compared with several other viral delivery systems. However, larger inserts may reduce viral production efficiency and should be evaluated during vector design and validation.
<h2 dir="ltr"> Specifications Context</h2>
 Modern research lentiviral vectors are commonly designed with self-inactivating (SIN) long terminal repeats (LTRs), an important biosafety feature that reduces promoter activity within the integrated viral LTR following transduction. Many lentiviral vector systems also incorporate regulatory elements that support efficient transgene expression and vector performance. Vector architecture, promoter selection, reporter configuration, and insert size can all influence downstream cloning, viral production, and expression outcomes. Product availability reflects MBP's catalogue as of mid-2026. 
The search for reliable lentiviral cloning vectors is over. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the perfect match today.

Cloning Vectors

Selection-Drug Killing Curve

Lenti-III-RFP-N Vector

LV076 by Applied Biological Materials is a 10 µg Lenti-III-RFP-N lentiviral expression vector with N-terminal RFP fusion tag and Puromycin selection (modified Lenti-III-HA backbone). For stable N-terminal RFP-fusion protein expression, subcellular localization, and anti-RFP IP. C-terminal variant: LV077. Requires packaging. Shipped on ice pack; store at −20°C or below.

Unit Quantity

Vector

Note

Shipping Condition

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Enhanced Lentivirus Safety Features-Replication Incompetency

Suggested MOI for Common Cancer Cell Lines

Lentiviral System FAQ

Lenti-III-UbC Expression Vector

G300 by Applied Biological Materials is a 10 µg Lenti-III-UbC lentiviral expression plasmid with a UbC (ubiquitin C) promoter driving transgene expression from within a multi-cloning site. Silencing-resistant promoter for stable expression in stem cells and CMV-silenced cell types. Modified Lenti-III-HA backbone. Requires lentiviral packaging. Store at −20°C or below.

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

pLenti-UbC-GFP Control Virus, LV027

Lenti-III-UBC-GFP Control Virus

LV027 by Applied Biological Materials is a pre-packaged UbC-promoter GFP lentiviral control virus supplied as 4 × 500 µL. Matched positive transduction control for UbC-driven lentiviral expression experiments. Ready to transduce. Note: 4–5 week production lead time. Research Use Only.

Vector Map

Titer

System

List of Markers

Promoter

Lead Time

<h2 data-section-id="xs82dy" data-start="121" data-end="167">What are additional immortalization agents?</h2>
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Additional immortalization agents cover recombinant lentiviral constructs delivering genes other than TERT or SV40 large T antigen used to bypass replicative senescence in primary cells, most notably HPV-16 E6/E7 (which inactivate p53 and Rb similarly to SV40T but are derived from human papillomavirus), Bmi1 (a polycomb group protein that represses the p16/CDKN2A senescence pathway), cMyc, CDK4, and HOXA/HOXB family genes. Related entities include p16/CDKN2A pathway repression (Bmi1), keratinocyte and epithelial cell immortalization (HPV E6/E7), polycomb group signaling, and combination immortalization strategies.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Lenti-HPV-16 E6/E7 degrades Rb and p53 for enhanced cell immortalization.</li>
<li dir="ltr" role="presentation">Lenti-Myc T58A &amp; Ras V12 enhance transformation and proliferation assays with high-titer mutant oncogenes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-p53 &amp; Rb siRNA Virus extends cellular longevity and targets the knockdown of tumor suppressors</li>
<li dir="ltr" role="presentation">Lenti-HOX Series (RFP Tagged) for lineage-specific expansion and includes high-titer vectors like HOXB8, HOXA9, and HOXA10 vectors.</li>
<li dir="ltr" role="presentation">EBV Virus (Wild-Type) for immortalization workflows of B-lymphocytes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-CDK4 &amp; Bmi1 Virus helps maintain proliferative capacity using cell mediators</li>
</ul>
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<h2 data-section-id="1acs4rs" data-start="1029" data-end="1087">How to choose additional immortalization agent products</h2>
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HPV E6/E7 for epithelial and keratinocyte immortalization
HPV-16 E6 and E7 proteins inactivate p53 and Rb respectively, similar in net effect to SV40 large T antigen but derived from a different viral source; E6/E7-based immortalization is particularly well-documented for keratinocytes and other epithelial cell types, and is sometimes preferred over SV40T for these lineages based on published precedent.
Bmi1 for stem and progenitor cell types
Bmi1 represses the p16/CDKN2A senescence pathway through its role in the polycomb repressive complex, and has been used to extend the proliferative capacity of hematopoietic and neural stem/progenitor cells in research settings; confirm the specific cell type has documented precedent for Bmi1-based extension before relying on it as a primary immortalization strategy.
cMyc and CDK4 as proliferation-driving factors
cMyc and CDK4 overexpression can drive proliferation and contribute to immortalization, often used in combination rather than as standalone immortalization agents; review the specific combination strategy in the source literature before ordering individual components.
E6/E7 separate vs. fused constructs
HPV E6/E7 may be delivered as separate genes or as a fused open reading frame; confirm which configuration a published protocol specifies, since this can affect relative expression levels of the two proteins.
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<h2 data-section-id="1fljvas" data-start="3287" data-end="3312">Specifications context</h2>
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Additional immortalization agent lentiviruses are typically supplied at titers around 1x10^8 TU/mL, shipped on dry ice and stored at -80&deg;C, with antibiotic or fluorescent-antibiotic fusion selection markers consistent with other immortalization lentivirus products. Pack sizes for academic labs commonly consist of small-volume aliquots sufficient for multiple transduction attempts, with constructs validated by sequencing and tested free of common contaminants.&nbsp;
This sub-category sits within MBP's cell immortalization catalog alongside TERT overexpression viruses and SV40T antigen overexpression viruses. Labs constructing custom combination vectors can also review MBP's cloning reagents. MBP's specialist team can help confirm which immortalization agent has documented precedent for a specific cell type before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to book high-quality immortalization vectors for your lab.

Additional Immortalization Agents

MSDS

Lentiviral Vector Amplification - Protocol

Lentivector Packaging - Protocol

Lentivirus Infection - Protocol

Cell Immortalization - Handbook

Cell Immortalization Reagents - FAQ

Lenti-Myc T58A Virus, High Titer

Lenti-Myc T58A Virus, High Titer (LV618) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing stabilized human Myc T58A mutant, supplied as 2 × 100 µl for immortalization of neural stem cells, primary kidney cells, erythroblasts, and epithelial cells. Shipped on dry ice; store at –80°C.

Application

Expression System Type

Caution

Lenti-p53 siRNA Virus, High Titer

Lenti-p53 siRNA Virus, High Titer (LV619) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing p53 siRNA for stable knockdown of the p53 tumor suppressor, supplied as 2 × 100 µl for cell immortalization and p53/TP53 pathway research. Shipped on dry ice; store at –80°C.

Lenti-PGK-Blank-CBh-GFP-2A-Puro Control Vector

Lenti-PGK-Blank-CBh-GFP-2A-Puro Control Vector Blank0616 (ABM) — 1.0 µg. Blank PGK lentiviral negative control with CBh-EmGFP-2A-Puro. GFP fluorescence for real-time transduction monitoring and FACS quantification; puromycin selection for stable cell line generation. Store −20 °C. RUO.

Lenti-PGK-Blank-CBh-RFP-2A-Puro Control Vector

Lenti-PGK-Blank-CBh-RFP-2A-Puro Control Vector Blank0617 (ABM) — 1.0 µg. Blank PGK promoter lentiviral negative control with CBh-driven RFP-2A-Puro selection marker. For controlling PGK-driven lentiviral experiments; puromycin selection confirms transduction, RFP visualizes transduced cells. Store −20 °C. RUO.

Lenti-PGK-Blank-SV40-Blast Control Vector

Lenti-PGK-Blank-SV40-Blast Control Vector (Blank0615) by Applied Biological Materials is a 1.0 µg insert-empty lentiviral plasmid with PGK promoter backbone and SV40-Blast (blasticidin) selection cassette, for use as a matched negative control in PGK-promoter lentiviral gene expression experiments. Store at –20°C or below.

Lenti-PGK-Blank-SV40-Hygro Control Vector

Blank0614 by Applied Biological Materials is a 1.0 µg blank lentiviral control vector with a PGK promoter (no insert) and SV40-driven Hygromycin resistance. Designed as the matched negative control for PGK-promoter lentiviral expression constructs using Hygromycin selection.

Lenti-PGK-Blank-SV40-Neo Control Vector

Lenti-PGK-Blank-SV40-Neo Control Vector (Blank0613) by Applied Biological Materials is a 1.0 µg insert-empty lentiviral plasmid with PGK promoter backbone and SV40-Neo selection cassette, for use as a matched negative control in PGK-promoter lentiviral gene expression experiments. Store at –20°C or below.

Lenti-Promoterless Vector

LV013 by Applied Biological Materials is a third-generation promoterless lentiviral transfer vector (10 µg). The MCS contains no upstream internal promoter, giving researchers full control to insert their own promoter, regulatory element, or inducible system for cell-type-specific or tightly regulated transgene expression from a stable lentiviral provirus. VSV-G compatible.

Applications

Lenti-Promoterless-Luc

LV092 by Applied Biological Materials is Lenti-Promoterless-Luc — lentiviral promoterless luciferase vector for quantitative BLI promoter activity assays. No upstream promoter; clone any candidate promoter upstream of luciferase. No-promoter BLI baseline control. GFP version: LV059. Requires packaging. Store at −20°C or below.