ABM Reagents

<h2 data-section-id="xs82dy" data-start="121" data-end="167">What are additional immortalization agents?</h2>
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Additional immortalization agents cover recombinant lentiviral constructs delivering genes other than TERT or SV40 large T antigen used to bypass replicative senescence in primary cells, most notably HPV-16 E6/E7 (which inactivate p53 and Rb similarly to SV40T but are derived from human papillomavirus), Bmi1 (a polycomb group protein that represses the p16/CDKN2A senescence pathway), cMyc, CDK4, and HOXA/HOXB family genes. Related entities include p16/CDKN2A pathway repression (Bmi1), keratinocyte and epithelial cell immortalization (HPV E6/E7), polycomb group signaling, and combination immortalization strategies.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Lenti-HPV-16 E6/E7 degrades Rb and p53 for enhanced cell immortalization.</li>
<li dir="ltr" role="presentation">Lenti-Myc T58A &amp; Ras V12 enhance transformation and proliferation assays with high-titer mutant oncogenes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-p53 &amp; Rb siRNA Virus extends cellular longevity and targets the knockdown of tumor suppressors</li>
<li dir="ltr" role="presentation">Lenti-HOX Series (RFP Tagged) for lineage-specific expansion and includes high-titer vectors like HOXB8, HOXA9, and HOXA10 vectors.</li>
<li dir="ltr" role="presentation">EBV Virus (Wild-Type) for immortalization workflows of B-lymphocytes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-CDK4 &amp; Bmi1 Virus helps maintain proliferative capacity using cell mediators</li>
</ul>
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<h2 data-section-id="1acs4rs" data-start="1029" data-end="1087">How to choose additional immortalization agent products</h2>
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HPV E6/E7 for epithelial and keratinocyte immortalization
HPV-16 E6 and E7 proteins inactivate p53 and Rb respectively, similar in net effect to SV40 large T antigen but derived from a different viral source; E6/E7-based immortalization is particularly well-documented for keratinocytes and other epithelial cell types, and is sometimes preferred over SV40T for these lineages based on published precedent.
Bmi1 for stem and progenitor cell types
Bmi1 represses the p16/CDKN2A senescence pathway through its role in the polycomb repressive complex, and has been used to extend the proliferative capacity of hematopoietic and neural stem/progenitor cells in research settings; confirm the specific cell type has documented precedent for Bmi1-based extension before relying on it as a primary immortalization strategy.
cMyc and CDK4 as proliferation-driving factors
cMyc and CDK4 overexpression can drive proliferation and contribute to immortalization, often used in combination rather than as standalone immortalization agents; review the specific combination strategy in the source literature before ordering individual components.
E6/E7 separate vs. fused constructs
HPV E6/E7 may be delivered as separate genes or as a fused open reading frame; confirm which configuration a published protocol specifies, since this can affect relative expression levels of the two proteins.
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<h2 data-section-id="1fljvas" data-start="3287" data-end="3312">Specifications context</h2>
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Additional immortalization agent lentiviruses are typically supplied at titers around 1x10^8 TU/mL, shipped on dry ice and stored at -80&deg;C, with antibiotic or fluorescent-antibiotic fusion selection markers consistent with other immortalization lentivirus products. Pack sizes for academic labs commonly consist of small-volume aliquots sufficient for multiple transduction attempts, with constructs validated by sequencing and tested free of common contaminants.&nbsp;
This sub-category sits within MBP's cell immortalization catalog alongside TERT overexpression viruses and SV40T antigen overexpression viruses. Labs constructing custom combination vectors can also review MBP's cloning reagents. MBP's specialist team can help confirm which immortalization agent has documented precedent for a specific cell type before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to book high-quality immortalization vectors for your lab.

Additional Immortalization Agents

MSDS

Lentiviral Vector Amplification - Protocol

Lentivector Packaging - Protocol

Lentivirus Infection - Protocol

Cell Immortalization - Handbook

Cell Immortalization Reagents - FAQ

Lenti-HPV-16 E6/E7 (Puro) Virus, High Titer

Lenti-HPV-16 E6/E7 (Puro) Virus, High Titer (LV617) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus co-expressing HPV-16 E6/E7 oncogenes and puromycin resistance, supplied as 2 × 100 µl for stable immortalization of primary human keratinocytes with puromycin selection. Shipped on dry ice; store at –80°C.

Unit Quantity

Application

Expression System Type

Vector Map

Note

Caution

Shipping Condition

<h2 data-section-id="1vscfio" data-start="222" data-end="262">What are TERT overexpression viruses?</h2>
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TERT overexpression viruses are recombinant lentiviral (or in some cases retroviral) particles carrying the human telomerase reverse transcriptase (hTERT) gene under a constitutive promoter, designed to extend telomere length and bypass replicative senescence in primary cells. Related entities include telomere maintenance, replicative senescence, population doubling limits, EF1-alpha and CMV promoters, and antibiotic/fluorescent selection markers (puromycin, hygromycin, GFP-Puro).
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Adeno-hTERT Antisense used in cancer research for inhibition of telomerase activity&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-hTERT Virus (High Titer) used as a standard tool for efficient immortalization of primary cells&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-EF1a-hTERT Virus uses the EF1a promoter for reliable, long-term expression.</li>
<li dir="ltr" role="presentation">Adeno-hTERT is used in transient TERT expression for high-efficiency adenoviral delivery</li>
<li dir="ltr" role="presentation">Lenti-hTERT is used as a streamlined vector for specialized genetic engineering.</li>
<li dir="ltr" role="presentation">Lenti-hTERT-2A-CDK4 Virus co-expression for complex cell types like myogenic cells.</li>
<li dir="ltr" role="presentation">Lenti-EF1a-hTERT-Hygro Virus for precise metabolic selection featuring hygromycin resistance&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-hTERT-Neo Virus for stable generation of cell lines, includes neomycin/G418 resistance&nbsp;</li>
</ul>
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<h2 data-section-id="t86qiz" data-start="932" data-end="983">How to choose TERT overexpression virus products</h2>
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Promoter selection
EF1-alpha promoter constructs are often preferred for primary cells where CMV promoter silencing over extended culture is a concern, though CMV-driven constructs offer strong initial expression; the choice may depend on the expected culture duration and cell type-specific promoter activity.
Selection marker type
Antibiotic resistance markers (puromycin, hygromycin, neomycin/G418, blasticidin) allow selection of stably transduced populations by drug treatment, while fluorescent-antibiotic fusion markers (e.g., GFP-Puro) additionally allow visual confirmation of expression and flow sorting; confirm the target cell line's existing markers don't conflict.
Titer and transduction efficiency
hTERT lentivirus is typically supplied at titers around 1x10^8 TU/mL; transduction efficiency depends on the target cell type's susceptibility to lentiviral infection, and some difficult-to-transduce primary cells may require optimization of multiplicity of infection (MOI) or use of transduction enhancers.
Species considerations
hTERT is human-specific; for non-human primary cells, confirm whether a species-matched TERT ortholog is available or whether SV40 large T antigen (with broader documented cross-species activity) is a more appropriate alternative for the target species.
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<h2 data-section-id="1fljvas" data-start="3151" data-end="3176">Specifications context</h2>
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TERT overexpression lentivirus is typically supplied at approximately 1x10^8 TU/mL in small-volume vials (e.g., 25 microliters per vial), shipped on dry ice and stored at -80&deg;C, with constructs validated by full-length sequencing and confirmed free of mycoplasma and common contaminants. Stable transduction and selection typically take 1-2 weeks before characterization, with full confirmation of telomere extension and extended proliferative capacity assessed over subsequent passages. As of 2026, hTERT lentivirus remains a standard tool for generating immortalized cell lines that retain much of the original primary cell's phenotype, distinguishing it from the more transformation-prone SV40 large T antigen approach.
This sub-category sits within MBP's cell immortalization catalog alongside SV40T antigen overexpression viruses and additional immortalization agents. Labs building custom expression constructs can also review MBP's cloning reagents for vector and competent cell options. MBP's specialist team can help confirm promoter and selection marker choices for a specific primary cell type before order placement.
Browse our collection and <a href="https://mbpinc.net/contact-us">contact the expert team at MBP</a> today to find premium-quality TERT viral vectors for your lab.&nbsp;

TERT Overexpression Viruses

Lenti-hTERT (No Selection Marker) virus, High Titer

Lenti-hTERT (No Selection Marker) Virus, High Titer (LV674) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing hTERT without a selection marker, supplied as 2 × 100 µl for stable genomic integration and long-term cell immortalization. Shipped on dry ice; store at –80°C.

Lenti-hTERT Virus, High Titer

Lenti-hTERT Virus, High Titer (LV615) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing hTERT with puromycin resistance, supplied as 2 × 100 µl for stable primary cell immortalization by telomere maintenance with puromycin selection. Shipped on dry ice; store at –80°C.

Lenti-hTERT-2A-CDK4 Virus, High Titer

Lenti-hTERT-2A-CDK4 Virus, High Titer (LV677) by Applied Biological Materials is a 10⁹ IU/ml bicistronic lentivirus co-expressing hTERT and CDK4 via 2A self-cleaving peptide, supplied as 2 × 100 µl for single-vector dual-pathway cell immortalization (telomere maintenance + pRb bypass). Shipped on dry ice; store at –80°C.

Lenti-hTERT-Neo Virus, High Titer

Lenti-hTERT-Neo Virus, High Titer (LV622) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing hTERT with neomycin (G418) resistance, supplied as 2 × 100 µl for stable integration and selection-based enrichment of immortalized primary cells. Shipped on dry ice; store at –80°C.

<h2 dir="ltr"> What Are Lentiviral Cloning Vectors?</h2>
 The transfer plasmid is the heart of every lentiviral construct &mdash; it defines the transgene cargo, promoter driving expression, selection marker, and structural LTR elements that enable stable genomic integration. Selecting the right cloning vector backbone is the first and most consequential decision in any lentiviral project.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li style="font-family: arial, helvetica, sans-serif;">Expression vectors featuring CMV, EF1&alpha;, PGK, and UbC promoter configurations for transgene expression studies</li>
<li>Fluorescent reporter vectors incorporating GFP, RFP, and CFP for cell tracking and visualization</li>
<li>Promoterless vectors for promoter characterization and regulatory element analysis</li>
<li>HA-tag and His-tag expression vectors for protein detection and purification workflows</li>
<li>Bicistronic and tricistronic vector systems for multi-gene expression applications</li>
<li>miRNA- and siRNA-compatible cloning vectors for RNA interference studies</li>
<li>Functional vectors including selectable-marker and HSVtk-based constructs for specialized research applications</li>
</ul>
<h2 dir="ltr"> How to Choose a Lentiviral Cloning Vector</h2>
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Vector Design
Expression vectors, reporter vectors, promoterless systems, tagged constructs, and multicistronic backbones are designed for different experimental objectives. Vector selection should be guided by the intended application, transgene design, and downstream analysis requirements.
Promoter Selection
Promoters influence the strength and pattern of transgene expression. Available vector configurations include CMV, EF1&alpha;, PGK, and UbC promoters, as well as promoterless backbones for studies focused on promoter activity and regulatory elements.
Reporter Requirements
Fluorescent reporters such as GFP, RFP, and CFP enable visualization of transduced cells, monitoring of expression, and assessment of transduction efficiency. Reporter selection should align with the imaging platform and experimental workflow.
Expression Configuration
Single-gene, bicistronic, and tricistronic vector designs provide different levels of flexibility for co-expression studies. Multi-cassette systems can simplify experiments requiring coordinated expression of multiple genes or reporter elements.
Insert Size Considerations
Lentiviral vectors can accommodate relatively large expression cassettes compared with several other viral delivery systems. However, larger inserts may reduce viral production efficiency and should be evaluated during vector design and validation.
<h2 dir="ltr"> Specifications Context</h2>
 Modern research lentiviral vectors are commonly designed with self-inactivating (SIN) long terminal repeats (LTRs), an important biosafety feature that reduces promoter activity within the integrated viral LTR following transduction. Many lentiviral vector systems also incorporate regulatory elements that support efficient transgene expression and vector performance. Vector architecture, promoter selection, reporter configuration, and insert size can all influence downstream cloning, viral production, and expression outcomes. Product availability reflects MBP's catalogue as of mid-2026. 
The search for reliable lentiviral cloning vectors is over. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the perfect match today.

Cloning Vectors

Selection-Drug Killing Curve

Lenti-III-2A-GFP Vector

LV070 by Applied Biological Materials is a third-generation lentiviral vector (10 µg) co-expressing a gene of interest and GFP via a T2A self-cleaving peptide (Thosea asigna) or separate promoter. T2A ribosomal skipping produces two separate proteins — no GFP fusion. Enables real-time fluorescence tracking and FACS enrichment of transgene-expressing cells without antibiotic selection. Store at &minus;20°C.

Vector

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Lenti-III-Blank Lentivirus

LVP687 by Applied Biological Materials is a pre-packaged Lenti-III-Blank lentiviral control virus. Blank insert, Lenti-III backbone. Supplied at 108 IU/mL as 3 × 250 µL. Matched high-titer negative control for Lenti-III series expression experiments. No reporter — confirm transduction by qPCR or parallel GFP control. Store at −80°C; shipped on dry ice.

Titer

Lenti-III-CFP-C Vector

LV669 by Applied Biological Materials is a third-generation lentiviral transfer vector (10 µg) with a C-terminal CFP fusion tag (excitation ~434 nm, emission ~477 nm) and Puromycin selection marker, from the Lenti-III-HA backbone series. Spectrally distinct from GFP, YFP, and RFP for multi-color imaging and FRET experiments. Shipped on Ice Pack; store at &minus;20°C.

Lenti-III-CFP-N Vector

LV668 by Applied Biological Materials is the Lenti-III-CFP-N Vector — 10 µg lentiviral plasmid for stable N-terminal CFP-tagged protein expression. Modified Lenti-III-HA backbone; Puromycin selection. CFP for cyan fluorescence tracking and FRET (donor). Requires packaging. Shipped on ice pack; store at −20°C or below.

Lenti-III-EF1a-GFP Control Vector

LV066 by Applied Biological Materials is a 1.0 µg EF1α-promoter lentiviral GFP control plasmid vector. Matched backbone control for EF1α-driven lentiviral expression experiments. Requires lentiviral packaging. For a ready-to-use pre-packaged version, see LV046. Store at −20°C or below.

Lentiviral System FAQ

Enhanced Lentivirus Safety Features-Replication Incompetency

Suggested MOI for Common Cancer Cell Lines

Lenti-III-EF1alpha-GFP Control Virus

LV046 by Applied Biological Materials is a pre-packaged EF1α-promoter GFP lentiviral control virus. Supplied at 107 IU/mL as 3 × 250 µL. Matched positive transduction control for EF1α-driven lentiviral expression experiments. Ready to transduce. Store at −80°C; shipped on dry ice.

Lenti-III-EF1α Vector

LV043 by Applied Biological Materials is a third-generation lentiviral transfer vector (10 µg) in the Lenti-III-HA backbone using an EF1&alpha; promoter instead of CMV for strong, constitutive transgene expression in cell types where CMV is silenced by methylation — including stem cells and iPSCs. Preferable to CMV for long-term silencing-resistant stable expression. Store at &minus;20°C.