Mammalian Cell Culture

<h2 data-section-id="pdko8n" data-start="0" data-end="50">What are recombinant proteins for cell culture?</h2>
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Recombinant proteins for cell culture are biologically active growth factors, cytokines, and signaling molecules produced using recombinant DNA technology to control specific cellular functions in vitro. Growth factors such as FGF (fibroblast growth factor), EGF (epidermal growth factor), and TGF-&beta; (transforming growth factor beta) regulate cell growth, proliferation, survival, and differentiation. Cytokines including IL-2, IL-7, IL-15, and IL-21 influence immune cell activation, expansion, and function. These proteins are widely used in serum-free and chemically defined media, stem cell and iPSC culture, immune cell expansion, organoid development, hybridoma production, and cell and gene therapy workflows where precise control over cellular signaling is required.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Includes cytokines, chemokines, growth factors, and signaling proteins to support proliferation, differentiation, and maintenance of&nbsp;mammalian cells</li>
<li>Rat, human, and mouse recombinant proteins compatible with translational research models and diverse mammalian cell culture&nbsp;</li>
<li>Optimizing cell metabolic activity, signalling pathways, and experimental reproducibility in vitro using recombinant proteins&nbsp;</li>
<li>Suitable for organoid development, expansion of cells, activation of immune cells, neuronal studies, and protein expression workflow optimization.</li>
</ul>
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<h2 data-section-id="4x59d8" data-start="1050" data-end="1118">How to choose recombinant proteins, cytokines, and growth factors</h2>
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Quality grade: RUO vs. preclinical vs. GMP
Research-use-only (RUO) proteins are suitable for most academic and industrial research applications. Preclinical-grade proteins provide enhanced documentation and quality control for studies supporting clinical development. GMP-grade proteins are manufactured under controlled quality systems with complete traceability and lot-to-lot bioactivity verification, making them appropriate for cell therapy, gene therapy, and other regulated applications.
Expression system and post-translational modifications
The production system can significantly affect protein activity. HEK293 and other mammalian expression systems preserve native human post-translational modifications such as glycosylation and phosphorylation, which are often necessary for full biological function. Bacterial expression systems such as E. coli may offer lower cost and higher yields but cannot reproduce many of these modifications.
Carrier-free formulation
Carrier-free recombinant proteins are formulated without stabilizing proteins such as bovine serum albumin (BSA). This eliminates potential experimental interference from carrier proteins and supports serum-free, animal-component-free, and GMP-compatible workflows. Carrier-free formulations are especially important when studying sensitive signaling pathways or developing clinical manufacturing processes.
Endotoxin level and purity
Endotoxins can stimulate inflammatory responses and alter cell behavior, particularly in immune cell cultures. For T-cell expansion, stem cell culture, and other sensitive applications, low-endotoxin or endotoxin-free proteins with documented purity above 95% are preferred to ensure reproducible results.
Stability format: standard vs. thermostable
Standard recombinant proteins gradually lose activity during prolonged incubation at 37&deg;C. Thermostable variants, such as engineered FGF2 formulations, maintain biological activity for longer periods and reduce the need for frequent media supplementation. These products can improve workflow efficiency in stem cell maintenance and differentiation protocols.
Storage and reconstitution
Lyophilized recombinant proteins offer extended shelf life and are typically stored frozen until use. After reconstitution, proteins should be aliquoted into single-use portions whenever possible to minimize repeated freeze-thaw cycles that can reduce activity. Proper storage conditions vary by product and should follow the manufacturer's recommendations.
<h2 data-section-id="rxnuuy" data-start="3659" data-end="3704">&nbsp;</h2>
<h2 data-section-id="1fljvas" data-start="4451" data-end="4476">Specifications context</h2>
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Modern recombinant cytokines and growth factors are available in a wide range of formats including carrier-free, low-endotoxin, animal-component-free, and GMP-grade formulations. Many GMP-grade cytokines such as IL-2, IL-7, IL-15, and IL-21 undergo lot-specific bioactivity testing using validated reference standards to ensure consistent biological performance. Mammalian-cell-expressed proteins are increasingly preferred for applications requiring native structure and maximum biological activity.
As of 2026, carrier-free, low-endotoxin recombinant proteins produced in mammalian expression systems have become the preferred choice for stem cell culture, immune cell expansion, organoid development, and advanced biomanufacturing workflows.
For basal media used alongside recombinant proteins, see Classic Media. For additional supplementation reagents, see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized recombinant proteins for your lab.

Recombinant Proteins

MSDS

Growth Factor-eFlyer

Growth Factors and Cytokines-Brochure

Growth Factors and Cytokines-FAQ

Role of Growth Factors in Cell Differentiation-Poster

Recombinant Mouse FGF2

Recombinant Mouse FGF2 (Basic FGF) Z200015 by ABM—tag-free, E. coli-expressed mitogen, 16.5 kDa, &gt;95% purity, ED50 ≤0.2 ng/mL. Available from MBP INC.

Organism

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Aliases

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Accession Number

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Reconstitution

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Shipping Condition

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Recombinant Mouse FGF2, 50 µg

Recombinant Mouse FGF2, 250 µg

Recombinant Mouse FGF2, 1.0 mg

Recombinant Mouse FGF8

Recombinant Mouse FGF8 by Applied Biological Materials — E. coli-expressed fibroblast growth factor with &gt;95% purity, ED50 0.05–0.5 ng/mL (NIH/3T3 proliferation), and endotoxin &lt;1.0 EU/µg. Available in multiple sizes for stem cell, neurodevelopment, and FGFR signaling research.

Recombinant Mouse FGF8, 50 µg

Recombinant Mouse FGF8, 1.0 mg

Recombinant Mouse FLT3 Ligand (FLT3LG)

Recombinant Mouse FLT3 Ligand (FLT3LG), catalog Z200055 by ABM. E. coli-expressed, 19 kDa, >95% purity, lyophilized. Bioactive FLT3/CD135 ligand, ED50 ~10 ng/mL. Endotoxin &lt;1.0 EU/µg.

Recombinant Mouse FLT3 Ligand (FLT3LG), 10 µg

Recombinant Mouse FLT3 Ligand (FLT3LG), 1.0 mg

Recombinant Mouse G-CSF (CSF3)

Recombinant Mouse G-CSF (CSF3) Z200605 by ABM — 19.2 kDa E. coli, &gt;95% pure by SDS-PAGE, ED(50) 10.0–60.0 pg/mL (cell proliferation assay), reconstitute at 100 µg/mL in sterile deionized water, &lt;1.0 EU/µg endotoxin. Ships from MBP INC to USA, Canada, and international labs.

Unit Quantity

Recombinant Mouse GH1

Z200065 by ABM — Recombinant Mouse GH1, 22 kDa, tag-free, expressed in E. coli. Purity &gt;95% by SDS-PAGE, endotoxin &lt;1.0 EU/µg, ED50 &lt;0.5 ng/mL (myoblast proliferation). Available in 50 µg, 200 µg, and 1.0 mg.

Recombinant Mouse GH1, 50 µg

Recombinant Mouse GH1, 200 µg

Recombinant Mouse GH1, 1.0 mg

Recombinant Mouse GM-CSF (CSF2)

Recombinant Mouse GM-CSF/CSF2 (Z200075) by ABM — 14 kDa E. coli-expressed, >95% pure, ED50 &lt;0.1 ng/mL, essential for BMDC/BMDM generation. Order from MBP INC.

Recombinant Mouse GM-CSF (CSF2), 20 µg

Recombinant Mouse GM-CSF (CSF2), 100 µg

Recombinant Mouse GM-CSF (CSF2), 1.0 mg

Recombinant Mouse GRO gamma (CXCL3)

Recombinant Mouse GRO gamma (CXCL3) — Z200415 by Applied Biological Materials. E. coli-expressed, &gt;95% pure by SDS-PAGE, 8 kDa, endotoxin &lt;1.0 EU/µg. CXCR2 ligand; chemoattracts 293/CXCR2 transfectants at 10–100 ng/mL. Available in 20 µg, 200 µg, and 1.0 mg.

Recombinant Mouse GRO gamma (CXCL3), 20 µg

Recombinant Mouse GRO gamma (CXCL3), 200 µg

Recombinant Mouse GRO gamma (CXCL3), 1.0 mg

Recombinant Mouse IFNG

Recombinant Mouse IFNG (Z200085) by ABM — 17 kDa, E. coli, Mouse (M. musculus), &gt;95% purity, tag-free. ED50 ≤1.0 ng/mL. Th1 macrophage activation and antiviral immunity research.

Recombinant Mouse IFNG, 100 µg

Recombinant Mouse IFNG, 1.0 mg

Recombinant Mouse IGF1

Z200625 by Applied Biological Materials (ABM) is a recombinant mouse IGF-1 expressed in E. coli, 7.9 kDa, with greater than 95% purity by SDS-PAGE, endotoxin below 1.0 EU/µg, and an ED50 below 0.1 ng/mL in mouse 3T3 cell proliferation assays. Shares 94% identity with human IGF-1.

Recombinant Mouse IL10

Recombinant Mouse IL10 (Z200115) by ABM — 19 kDa E. coli-expressed anti-inflammatory cytokine, >95% pure, ED50 &lt;2.0 ng/mL, CSIF/If2a. Order from MBP INC.

Recombinant Mouse IL10, 10 µg

Recombinant Mouse IL10, 1.0 mg

Recombinant Mouse IL11

Z200125 by Applied Biological Materials is recombinant mouse IL11 (M. musculus), a tag-free cytokine expressed in E. coli. At 19 kDa with &gt;95% purity, endotoxin &lt;1.0 EU/µg, and ED50 &lt;0.5 ng/mL, this gp130 pathway ligand supports hematopoiesis, bone density maintenance, and mucosal protection assays. Lyophilized from 0.2 µm filtered PBS.

Recombinant Mouse IL11, 10 µg

Recombinant Mouse IL11, 100 µg

Recombinant Mouse IL11, 1.0 mg

Flyer

Brochure

Recombinant Mouse IL13

Recombinant Mouse IL-13 by Applied Biological Materials — E. coli-expressed Th2 cytokine with &gt;95% purity, ED50 &le; 2.0 ng/mL (TF-1 proliferation), and endotoxin &lt;1.0 EU/µg. Available in multiple sizes for allergy, fibrosis, and macrophage polarization research.