Mammalian Cell Culture

<h2>What is PriGrow Media?</h2>
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PriGrow Media is a proprietary series of basal cell culture media developed and distributed by MBP in partnership with ABM (Applied Biological Materials), where each individual PriGrow formulation, such as PriGrow I, PriGrow IV, or PriGrow X, has a distinct composition of HEPES and bicarbonate buffers, nutrient concentrations, and basal supplements specifically optimized to support the growth of a defined set of specialized cell lines or primary cell types. Unlike standard media like DMEM or RPMI that are broadly formulated for a wide range of cell lines, each PriGrow formulation is a targeted solution for cell types that do not grow well, or grow inconsistently, in standard mammalian cell culture media.
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<h2 dir="ltr">What you will find:</h2>
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Endothelial and epithelial formulations optimized to preserve critical barrier functions and&nbsp;unique physiological phenotypes in specialized vascular cell lines.
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Myogenic media for the smooth and skeletal muscle cells in vitro proliferation, functional maintenance, and structural preservation
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Hepatic induction systems for supporting liver cell research, drug metabolism profiling, and long-term functional assays.
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Serum-free, chemically defined solutions for regulation of multi-lineage differentiation, progenitor line expansion, and high-yield recovery after thawing.
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<h2>How to use PriGrow Media</h2>
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Identify the correct PriGrow variant for your cell type
Each MBP primary or specialized cell line product lists the recommended PriGrow variant in its data sheet. Using the wrong variant may result in suboptimal growth or inconsistent results, so matching the variant to the cell type is the critical first step.
Add FBS at 10% to make the basal PriGrow medium into complete media
PriGrow media are supplied as serum-free basal media and must be supplemented with 10% fetal bovine serum to provide the growth factors, carrier proteins, and lipid supplements that cells require for robust growth. Simply adding FBS at 10% of the final volume to the PriGrow basal medium produces complete media ready for use.
Optionally add antibiotics to complete PriGrow media
Penicillin-streptomycin with or without amphotericin B can be added to complete PriGrow media to reduce contamination risk, though antibiotic-free culture is standard in many labs to avoid masking low-level contamination and maintain cell health in long-term culture.
Warm PriGrow media to 37&deg;C before adding to cells
Like all cell culture media, PriGrow basal media and complete PriGrow media should be pre-warmed in a water bath at 37&deg;C before use to avoid cold-shock stress on cells that could affect growth and viability during media changes or passaging.
Maintain cultures at 37&deg;C under 5% CO₂
PriGrow media are formulated for standard mammalian cell culture conditions of 37&deg;C and 5% CO₂, which maintains physiological pH in the bicarbonate-buffered medium, unless the specific cell type protocol calls for different conditions.
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<h2>Specifications context</h2>
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PriGrow media contain phenol red as a standard pH indicator and are provided as serum-free basal media, with each formulation's HEPES and bicarbonate buffer ratios, nutrient levels, and supplemental additives matched to the cell types it is optimized for. As of 2026, specialized primary cell media like PriGrow remain a distinct product category from standard broad-spectrum media, since primary cells and cell-type-specific in vitro models require media compositions that better match in vivo nutritional conditions than DMEM or RPMI provides.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-quality PriGrow media for your lab.

PriGrow Media

TM001-MSDS

TM001-JRP030-6-Certificate of Analysis\n

TM001-JRR054-6-Certificate of Analysis

Prigrow I Medium

TM001 by ABM (Applied Biological Materials) — PriGrow I Medium, 500 mL serum-free and antibiotic-free basal cell culture medium. pH 7.15–7.25, osmolarity 270–310 mOsm/kg H₂O, endotoxin ≤1 EU/mL. Lot-tested for sterility, pH, osmolality, endotoxin, and cell performance. Store at 2–8°C.

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MSDS

Prigrow II Medium

Prigrow II Medium (TM002) by Applied Biological Materials (ABM) is a 500 mL serum-free, antibiotic-free cell culture medium for primary mammalian cells. pH 7.0–7.6, osmolarity 264–292 mOsm/kg, endotoxin ≤1 EU/mL. QC tested for sterility, pH, osmolarity, endotoxin, adherence rate, morphology, and metabolic activity. Stored at 2–8°C.

TM003 - MSDS

Prigrow III Medium

TM003 by Applied Biological Materials is Prigrow III Medium (500 mL), a serum-free, antibiotic-free mammalian cell culture medium with lot-tested pH 7.0–7.4, osmolarity 315–350 mOsm/kg, and endotoxin ≤1 EU/mL — qualified per lot for sterility, morphology, and cellular metabolism.

Prigrow IV Medium

PriGrow IV Medium (TM004) by Applied Biological Materials is a serum-free, antibiotic-free specialty basal medium for immortalized human endometrial stromal cells, with pH 7.0–7.6, osmolarity 287–317 mOsm/kg, and endotoxin &le; 1 EU/mL.

Prigrow IX Medium

TM019 by Applied Biological Materials is Prigrow IX Medium, a 500 mL serum-free, antibiotic-free basal medium with phenol red, for mammalian primary cell culture — lot-confirmed pH 7.0–7.4, osmolarity 290–318 mOsm/kgH₂O, and endotoxin ≤1 EU/mL.

Prigrow V Medium

Prigrow V Medium TM015 by ABM: 500 ml serum-free, antibiotic-free basal medium for mammalian cell culture. pH 6.95–7.15, osmolarity 270–305 mOsm/kgH2O, endotoxin ≤0.25 EU/ml. Lot-tested for sterility, pH, osmolality, and endotoxin. Store at 2–8°C. Research use only.

Prigrow VI Medium

TM016 by Applied Biological Materials is Prigrow VI Medium (500 mL), a serum-free, antibiotic-free mammalian cell culture medium with lot-tested pH 7.0–7.4, osmolarity 306–346 mOsm/kg, and endotoxin ≤0.05 EU/mL — qualified per lot for sterility, morphology, and cellular metabolism.

Prigrow VII Medium

TM017 by Applied Biological Materials is Prigrow VII Medium, a 500 mL serum-free, antibiotic-free basal medium for mammalian primary cell culture, with lot-confirmed pH 6.9–7.3, osmolarity 280–308 mOsm/kgH₂O, and endotoxin ≤0.25 EU/mL.

Prigrow VIII Medium

TM018 by Applied Biological Materials is Prigrow VIII Medium, a 500 mL serum-free, antibiotic-free basal medium for mammalian primary cell culture, with lot-confirmed pH 6.91–7.27, osmolarity 281–317 mOsm/kgH₂O, and endotoxin ≤1 EU/mL.

Prigrow X.1 Medium

Prigrow X.1 Medium TM010 by ABM: 500 ml protein-free, antibiotic-free medium for corneal progenitor cells, corneal epithelial cells, and keratinocytes. BPE + 0.07 mM calcium supplement. Basal at 4–8°C, supplement at −20°C; stable 30 days post-mix. Lot-tested for adherence, sterility, pH, osmolality, and endotoxin.

Prigrow XI Medium

Prigrow XI Medium (TM011) by Applied Biological Materials (ABM) is a 450 mL sterile, clear cell culture medium with endotoxin ≤1 EU/mL, stored at 2–8°C and shipped on ice packs. Part of ABM's PriGrow series of specialised cell culture media.

Prigrow XII Medium

TM012 by Applied Biological Materials (ABM) is Prigrow XII Medium, a 500 mL serum-free and antibiotic-free liquid medium for primary mammalian cell culture, with pH 7.0–7.6, osmolarity 282–312 mOsm/kgH₂O, and endotoxin ≤1 EU/mL. Each lot is tested for sterility, pH, osmolality, and cellular performance.