A 96-well deep well plate is a type of laboratory plate that is based on 96-well deep microplates but has deeper wells to increase the space of each well. They are the centerpiece in labs and research centers nationwide that should be more frequently addressed for both short- and long-term sample storage and are the best option for preparation and mixing. Although their applications are many, they are commonly used in research and life science applications such as enzyme assays, reagent reservoirs, and secure sample storage and proteomics studies.
However, working with and loading the 96 deep-well storage plate is an open challenge. Your mind can start to trick you no matter how many pipettes or plates you have loaded for your research or work. So, here we will talk about some tips and techniques when using a 96-Deep Well Plate which you can start including in your lab routine.
1.   Match Your Pipette Tips To The Matching Wells
For deep well plates, a new pipette box is the best. As you go, match well to the boxes. In case of running out, have a backup box on reserve to get rid of any damage if you want more. To keep a well-count track, use pipette tips.
2.   Note It In Excel
Prepare an excel sheet to make 96 deep well plate maps and the master mix. For primers and samples, each well has a name. Logically put up all your master mixes, and if you are using multiple primer sets, color code for each of them. Whenever you come to the lab, always bring this sheet with you, and mark it off in the sheet as you go.
Alternatively, you can write the reagent amounts on a sticky note and use that as your sample key while loading. Select a system to go through them, e.g., numerically or alphabetically, depending on how they’re organized, and stick to it at all costs. To prepare the mix, arrange everything on your rack in the correct order, then, after finishing, transfer it to the furthest corner.
3.   Tape Off The Complete Plate
Tape off the whole plate, excluding the area where you are loading actively. This way works across the plate; move the tape every time a section is done. To stay on track, you can give labeling to your tape (like 1 – 12 or A – H).
For instance, First get the tape and slowly cover columns 3 and 4 while loading Gene A master mix into columns 1 and 2 of your deep well plate. To keep organized, you can also perform this single column at a time which helps keep you focused in tough middle wells. Note steadily hold down the plate when removing your tape for no splashing.
Conclusion
Here are some significant Tips and techniques for overcoming 96 Deep Well Plate Mistakes. Working with deep well plates is a sensitive task due to the multiple wells involved, which can be tricky and complex while loading. Following these tips will be highly beneficial for you.
MBP Inc. is a high-quality molecular biology products provider. Our product ranges are 2.0 ml Deep Well Plates for Hamilton, pipette tips, etc. Get in touch with us or visit our website to learn more about our products.
