Name: Yeast Nitrogen Base (YNB) with Ammonium Sulphate, without Thiamine HCl
Brand: Molecular Depot
SKU: B2025801
Price: 495.00 USD
Availability: InStock

<h2 data-section-id="177zc5g" data-start="0" data-end="42">What is yeast and fungal culture media?</h2>
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Culture media in this subcategory are the specific growth formulations used for yeast and fungal work, including YPD for routine propagation, Sabouraud dextrose agar for isolating yeasts and molds, and synthetic defined (SD) media built from a minimal base for auxotrophic selection in yeast genetics. This is narrower than the parent Yeast &amp; Fungal Culture category, which also covers dropout supplements and transformation reagents; this subcategory is specifically the growth media itself.
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<h2 dir="ltr">What you will find: </h2>
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Enriched Proliferation Blends: Precision formulations adjusted with active growth modifiers to drive rapid cell division and maximize organogenesis across diverse target cultures.
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High-Energy Nutrient Bases: Calibrated carbon and carbohydrate matrices prepared to sustain the heavy metabolic demands of rapidly multiplying tissue structures.
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Proportionate Essential Salts: Balanced macro- and micronutrient combinations optimized to prevent tissue exhaustion and maintain excellent cellular health during intense growth phases.
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<h2 data-section-id="z8b1xq" data-start="680" data-end="741">How to choose a yeast and fungal culture media formulation</h2>
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Match the formulation to routine growth or genetics work
YPD suits routine propagation of most yeast species without genetic selection, while SD base paired with the correct dropout supplement is needed when a protocol calls for auxotrophic selection of transformants.
Confirm whether the workflow needs fungal isolation rather than propagation
Sabouraud dextrose agar's acidic pH and high dextrose concentration favor fungal growth while suppressing many bacteria, which makes it the standard choice for isolating fungi from mixed or environmental samples rather than propagating a known strain.
Decide on antibiotic-supplemented plates for contaminated samples
Sabouraud dextrose agar formulations with gentamicin and chloramphenicol further suppress bacterial growth when isolating fungi from non-sterile clinical or environmental specimens, at typical concentrations around 50 &micro;g/mL each.
Choose liquid, agar, or powder format for the batch size
Powdered media suits labs preparing larger batches themselves, while pre-poured agar plates save preparation time for routine isolation, maintenance, or screening work.
Confirm sporulation or differential media needs
Some genetics protocols require a separate sporulation medium to induce meiosis in diploid strains, which has a different nutrient composition than standard vegetative growth media like YPD. Selective and sporulation media formulations are designed specifically for these workflows.
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<h2 data-section-id="1fljvas" data-start="2856" data-end="2881">Specifications context</h2>
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Sabouraud dextrose agar is recommended by the European Pharmacopoeia for isolating Candida albicans from non-sterile specimens, and off-the-shelf formulations with gentamicin (50 &micro;g/mL) and chloramphenicol (50 &micro;g/mL) are common for environmental and clinical-adjacent research isolation work. As of 2026, synthetic defined media paired with dropout supplements remains the standard approach for auxotrophic selection in yeast genetics, distinct from the undefined, complete formulations used for routine propagation.
<a href="https://mbpinc.net/contact-us">Request a specialized bulk quote</a> from the MBP sales desk today to support your high-volume cultivation goals.

Culture Media

Molecular Depot

Key Specifications

Yeast Nitrogen Base (YNB) with Ammonium Sulphate, without Thiamine HCl

Yeast Nitrogen Base (YNB) with Ammonium Sulphate, without Thiamine HCl (B2025801) from Molecular Depot is yeast nitrogen base with ammonium sulphate, without thiamine HCl, a microbiological medium component. Yeast nitrogen base with ammonium sulphate, without thiamine HCl is used to prepare defined synthetic media for yeast culture, selection and nutrient limitation experiments. Available from MBP, a registered vendor at leading US research institutions.