Name: ΦX174 RF DNA
Brand: Molecular Depot
SKU: B2016430
Price: 695.00 USD
Availability: InStock

<h2 dir="ltr">What Are Molecular Biology Reagents?</h2>
 Molecular biology forms the technical foundation of virtually every life-science research workflow &mdash; from gene expression analysis and genotyping to cloning, sequencing, and functional genomics. MBP Inc.'s molecular biology category centers on PCR reagents and kits (standard Taq, hot-start, high-fidelity, and master mix formats), alongside cloning enzymes, nucleic acid purification kits, and gel electrophoresis supplies. Reliable molecular biology workflows depend on high-quality reagents and kits. Select the products best suited to your research and analytical requirements.
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<h2 dir="ltr">What you will find:</h2>
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<a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors" title="Enzymes and Inhibitors:">Enzymes and Inhibitors:</a> Highly stable ligases, Zymolyase&trade; enzymes, and RNase inhibitors optimized for payload preservation and preventing template degradation.
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<a href="https://mbpinc.net/reagents/molecular-biology/pcr-reagents-and-kits" title="PCR Reagents &amp; Kits:">PCR Reagents &amp; Kits:</a> High-fidelity master mixes and magnetic-bead systems like Magbio HighPrep PCR to minimize sequence bias while ensuring high-sensitivity amplification.
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<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-cleanup-kits" title="Nucleic Acid Cleanup Kits:">Nucleic Acid Cleanup Kits:</a> Specialized tools such as the Zymoclean&trade; Gel RNA Recovery and RNA Clean &amp; Concentrator&trade; kits for removing primers and salts to provide transfection-grade purity.
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Nucleic Acids, Libraries &amp; Arrays: Dependable molecular standards, oligonucleotides, and structural building blocks calibrated for consistent genomic workflows.
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<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits" title="Nucleic Acid Extraction Kits:">Nucleic Acid Extraction Kits:</a> Advanced silica-column and magnetic bead technologies, including Magbio HighPrep and Quick-DNA/RNA&trade; kits, for rapid isolation from tissue, blood, viral, plant, or FFPE samples.
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<h2 dir="ltr">How to Choose Molecular Biology Reagents</h2>
 Application Type Routine genotyping and colony screening suit standard Taq PCR; specificity-sensitive applications (low-abundance targets, multiplex PCR) benefit from hot-start formulations; cloning, sequencing, and site-directed mutagenesis require high-fidelity proofreading polymerases. Format Convenience 2x master mixes pre-combine polymerase, dNTPs, buffer, and MgCl2 into a single tube, requiring only template and primers &mdash; ideal for high-throughput workflows. Individual enzyme and buffer components suit custom protocol optimisation. Fidelity Requirements Standard Taq has no proofreading activity and suits applications tolerant of occasional misincorporation. High-fidelity polymerases (Phusion, Q5, KOD, Pfu equivalents) reduce error rates roughly 50-100-fold, essential for cloning into expression vectors or sequencing-bound amplicons. Specificity Needs Hot-start formulations (antibody-mediated or chemically modified) remain inactive at room temperature during reaction setup, preventing non-specific primer extension and primer dimer formation common with standard Taq. Throughput and Scale High-throughput genotyping and screening favour ready-to-use 2x master mixes with consistent lot-to-lot performance; lower-throughput custom work may favour individual component flexibility. 
<h2 dir="ltr"> Specifications Context </h2>
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Molecular biology workflows depend on high-quality reagents for reliable performance in PCR, cloning, sequencing, and gene expression applications. Reagent selection directly impacts yield, specificity, and downstream reproducibility. As of 2026, kit-based workflows and master mixes are widely used to reduce handling variation and improve consistency in high-throughput experiments.&nbsp;Most enzymes, PCR/qPCR reagents, and nucleic acid kits require &minus;20 &deg;C storage to maintain stability, and lot-specific quality control data (CoA) is commonly used to ensure batch-to-batch consistency. Standardizing extraction, cleanup, and amplification reagents across workflows helps reduce variability and simplifies troubleshooting in multi-step experimental designs.&nbsp;Product availability reflects MBP&rsquo;s catalogue as of mid-2026.
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Contact our <a href="https://mbpinc.net/contact-us" title="expert team at MBP" style="color: #236fa1;">expert team at MBP</a> today to request a quote tailored to your laboratory requirements.

Molecular Biology

Molecular Depot

Key Specifications

ΦX174 RF DNA

ΦX174 RF DNA by Molecular Depot is a bacteriophage phiX174 replicative form DNA, the double-stranded circular intermediate of the phage genome. It is supplied as a solution and stored at -20°C. It is used as a substrate in restriction digestion and sequencing, as a size standard and control template and in molecular biology research. Each unit contains 50 µg. MBP supplies Molecular Depot research reagents to research labs in the United States, Canada and worldwide.