Researchers use cell culture plates in laboratories to develop optimal conditions for cell culture. So, cell culture plates are ideal for the cell cultures’ growth. These plates or dishes are available in different shapes, including flat, V-shaped, or round at the base. These are transparent plates, and this attribute allows visual examination. These plates often have lids that help researchers protect the samples and place them in multiple wells for storage and research.
This blog focus on one of the essential molecular bioproducts for labs, cell culture plates. So, we discuss the tissue culture, treated and untreated cell culture plates and their different types.
Cell Culture Plates And Tissue Culture
The term ‘tissue culture’ is interchangeably used for cell cultures and organs. Cell culture excludes cells from a plant or animal and ensures their later growth in an optimum simulated environment.
Tissue culture refers to a method of growing tissue in a culture medium that is outside the organism (plant or animal). Culture plates are the choice formats when researchers need direct access to cells. Sterility of the cell culture plates used in the process is necessary.
An optically transparent, nontoxic and biologically inert surface is the requirement for anchorage-dependent cells, enabling cells to attach and grow. Cell culture plates are among the most critical group of molecular bioproducts often used in modern lab settings.
The plates are designed to make handling and stacking performance easier. So, they are safe to use in labs. The plate has a handling ring that ensures safety during the cells’ expansion and transportation.
Every stage of cell culture depends on the cell culture plates that deliver the most authentic and reproducible results. In addition, the uses and applications of bioscience have been growing at a fast pace. These plates have thus become one of the essential lab equipment that meets uncountable bio-production demands generated worldwide. The surface of cell culture plates has evolved throughout the last century. These plates are available in various sizes, formats, and surfaces.
Cell culture plates allow researchers to perform their work with utmost confidence. They often use the lot-to-lot cell line testing technique to make sure consistency. It all means cell culture plates must be of excellent optical quality to get reproducible results and healthy cells. Â
Researchers use some specialized cell culture plates for mammalian cell growth. Other dishes are available for bacterial culture. Generally, manufacturers treat cell culture plates with the Vacuum Plasma method to get a reliable and hydrophilic surface for cell attachment. The flat growth surfaces help researchers maximize usable area for growth and transparency.
The Difference Between Treated And Untreated Cell Culture Plates
The primary purpose of cell culture plates is to ensure cell integrity. Thus, researchers worldwide prefer these plates and consider them ideal for cell harvesting and culture. There is a hydrophobic surface in untreated cell culture plates. The plasma-treated plates become hydrophilic, allowing cells to attach and grow.
While hydrophilic surfaces have a strong affinity for water, hydrophobic surfaces resist or fail to amalgam with water.
Types Of Cell Culture PlatesÂ
- High-Clarity Polystyrene Cell Culture Plates – These plates have various capacities: 35, 60, 100, and 150 mm. These plates ensure distortion-free observation, as each has a flat transparent surface. They are available in both treated and non-treated conditions.
- The Vacuum Plasma Tissue Culture Plates – These plates with 100 mm capacity and gripping rings are ideal for excellent cell adherence. They are available in easy-tear sterile PA/PE packaging. Their handling and storage are easy, as they are stackable.
- Coverslip Preparation Plates – These plates come with tweezers and gas exchange vents. They are non-Pyrogenic, meaning they do not produce heat when inserted into the organ in diagnostic medical imaging.Â
Final Words
One of the most crucial cellular and molecular biology techniques is cell culture since it helps researchers investigate the biology, biochemistry, physiology, and metabolic processes of organs’ cells and diseased cells. In this process, the role of cell culture plates is pivotal. These plates preserve cells by preventing contamination that may cause morphological or physiological changes.
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