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Accurate pipetting is necessary to carry out efficient experiments. The use of pipettes requires adequate knowledge and discretion. The lack of understanding and techniques of pipetting can result in poor turnout. The cell culture experiment has many chances of contamination during the procedure. Consequently, the inaccuracies lead to unfavourable conditions, thereby affecting the final result.
Contamination is the most significant factor that influences the outcome of the cell culture. The technicians and scientists must follow a pattern that prevents defilement and keeps the cells intact. This way, the experiment will no longer be affected by the potential damages.
Principle Of Cell Culture:
The foremost principle of cell culture is the aseptic technique. This technique protects the cells from catching bacteria and germs. It is the most significant technique because, without it, the cells can be severely affected. The aseptic technique is the only method of keeping the cells safe and free from contamination. Contaminated cells can change the subject of the study because they lose their original state. Their properties and functions become different and change quickly. Subsequently, it provides an inappropriate result than anticipated. The most common contaminants that the scientists come across are the following:- Bacteria
- Yeast
- Mould
- Plastic leachables
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Pipette-to-sample Contamination:
- Try to use hygienic and sterilized filter tips to prevent the pipette from catching germs.
- Use a separate pipette for each sample.
- Autoclave the pipette or use ethanol for sanitization.
- Try to select autoclave compatible pipettes.
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Sample-to-pipette Contamination:
- The use of filter tips can prevent the sample from getting inside the pipette.
- The most suitable position of pipetting is vertical.
- Store the pipettes in an upright position.
- Make sure to lean the bottle for effective pipetting.
- Release the button slowly while pipetting. It’ll keep the sample from entering into the pipette.
- Use a pipette that comes with a soft ejection feature.
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Sample-to-sample Contamination:
- Do not use the same tip for multiple samples.
- Ensure that the pipette is properly sterilized.
- Check that the pipette is leak-proof and contains the sample safely.
It is vital to use separate and pre-sterilized filter tips to avoid any potential damage to the cells. It is the most convenient method to conduct the experiment efficiently. Moreover, the quality of pipette tips greatly affects the contamination. Therefore, it is necessary not to compromise on the material and quality of the pipettes.
Tips For Scientists To Avoid Contamination: It is of crucial importance that scientists have adequate knowledge and information related to avoiding contamination. They must be aware of the procedures and techniques relevant to the experiment. This way, the chances of any errors and defects throughout the procedures become fewer.
The following tips are beneficial for scientists.
- Efficient laboratory practice.
- Planning of the future steps and management.
- Having all the essential products necessary for the experiment.
- Prioritizing aseptically technique.
To sum up, by following the tips above, the contamination of cells becomes avoidable. Another significant factor attached to this experiment is using high-quality products. Molecular Biology Products (MBP INC) is a reliable online dealer of laboratory products and Molecular Biology Kits. We aim to provide products that meet global standards. Our team responds to all the queries of the customers and offers them unmatchable services.
Citation: Carter. C. (October 1st, 2019). Select Science: Ways to avoid cell contamination: Pipetting tips you need to know. Retrieved August 4th, 2021 from https://www.selectscience.net/editorial-articles/ways-to-avoid-cell-contamination-pipetting-tips-you-need-to-know/?artID=49826
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