Name: Steroid Free Human Serum
Brand: Molecular Depot
SKU: B2015071
Price: 995.00 USD
Availability: InStock

<h2 data-section-id="u0rtwb" data-start="0" data-end="39">What is the Serum &amp; Plasma category?</h2>
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Serum &amp; Plasma covers fetal bovine serum (FBS, also called fetal calf serum/FCS) &mdash; the most widely used growth supplement for mammalian cell culture media &mdash; along with related plasma products. FBS supplies a complex mixture of growth factors, proteins, trace elements, vitamins, and hormones that basal media (DMEM, RPMI, and others) entirely lack, making it the default supplement at typically 10% (v/v) across mammalian cell culture protocols. FBS quality is differentiated by manufacturing grade (standard/Premium through Premium Select), treatment (heat-inactivated, gamma-irradiated), origin certification (USDA-approved), and the depth of lot-specific testing documented in the Certificate of Analysis.
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<h2 dir="ltr">What you will find:</h2>
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Cell culture media supplementation using Fetal Bovine Serum (FBS)&nbsp;
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Prevent complement-mediated cell lysis using Heat-inactivated animal serum&nbsp;
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Translational and immunology research using Sterile human plasma derivatives&nbsp;
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Sensitive embryonic stem cell propagation using Endotoxin-free serum lots&nbsp;
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<h2 data-section-id="1nxdu4e" data-start="1082" data-end="1124">How to choose serum and plasma products</h2>
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FBS grade tier
Premium FBS is sterility-, virus-, and performance-tested with a biochemical profile, demonstrating excellent performance across a broad spectrum of cell types &mdash; the standard choice for most research. Premium Select represents the highest available grade: lowest endotoxin and hemoglobin levels, plus hormone analysis, suitable for the most demanding and sensitive cell lines where standard Premium FBS may show batch-to-batch performance variation. Specialty grades are optimized for specific applications &mdash; hybridoma culture, embryonic stem cells, Tet-On/Tet-Off tetracycline-controlled expression systems, and hormone-dependent cell research (insulin- and estrogen-related studies) where standard FBS hormone content could confound results.
Heat inactivation: when it's appropriate
Heat inactivation (56&deg;C for 30 minutes) destroys complement proteins &mdash; necessary for immunological applications where active complement would interfere (e.g., complement-mediated cytotoxicity assays). The trade-off: heat inactivation generally reduces growth-promoting properties and increases crystalline/flocculent precipitate formation (salts, proteins, lipids, fibrin). Choose standard FBS for routine culture; choose heat-inactivated specifically when complement inactivation is a documented requirement of your assay.
Gamma irradiation for biosecurity
Gamma-irradiated FBS provides an additional layer of viral inactivation beyond filtration and standard testing, selected when research requires the highest level of biosecurity for safer, more consistent outcomes. This addresses a different concern than heat inactivation (viral vs. complement) and can be combined with other treatments depending on product line.
USDA-approved origin and traceability
USDA-approved testing and origin certification verifies virus and disease safety of source material for US import, and USDA APHIS certification enables US-origin serum use in products manufactured for export. Collection and processing under USDA requirements ensures traceability back to collection date and location &mdash; relevant documentation for regulatory pathways toward cell therapy manufacturing. Characterized FBS products extend this with traceability back to original source, sometimes with additional independent third-party verification.
Quality testing depth
Standard quality testing covers sterility (no detectable bacterial/fungal growth per current USP), mycoplasma (negative), virus panel (per 9 CFR 113.53 for USDA-regulated products), endotoxin (e.g., &lt;0.1 EU/mL), and hemoglobin (e.g., &lt;15 mg/dL, indicating collection cleanliness). Premium-tier products add hormone analysis and biochemical profiling (gamma globulin, glucose, IgG).
Manufacturing process and filtration
Quality manufacturing includes closed-system collection, positive-pressure HEPA-filtered cleanroom processing, and triple sequential 0.1 &micro;m (100 nm) filtration, documented within ISO 9001:2015-certified facilities.
Storage and handling
FBS requires frozen storage (-20&deg;C) with documented freeze/thaw/storage protocols to preserve growth performance consistency. Once thawed, store at 2&ndash;8&deg;C short-term and avoid repeated freeze-thaw cycles &mdash; aliquot into single-use volumes after first thaw. "One Shot" small-bottle formats (e.g., 50 mL) eliminate aliquoting and the freeze-thaw cycle entirely.
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<h2 data-section-id="1fljvas" data-start="5263" data-end="5288">Specifications context</h2>
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Certified FBS at &lt;0.1 EU/mL endotoxin and &lt;15 mg/dL hemoglobin represents documented quality benchmarks from commercial suppliers. Triple 0.1 &micro;m sequential filtration and closed-system, HEPA-filtered cleanroom processing under ISO 9001:2015 are standard manufacturing documentation for quality-tier FBS. As of 2026, characterized FBS with full source traceability (including third-party verification options) is increasingly specified for translational research labs anticipating eventual transition toward regulated cell therapy pathways.
For human-derived xeno-free alternatives to FBS, see Blood Products &amp; Biofluids (human platelet lysate). For individual growth factors and hormones, see Growth Factors &amp; Hormones.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP Inc. and get high-purity serum and plasma reagents for your lab.

Serum & Plasma

Molecular Depot

Key Specifications

Steroid Free Human Serum

Steroid Free Human Serum by Molecular Depot is a human serum from which steroids have been removed, delipidated and heat inactivated. A steroid-stripped serum provides the full serum background without the endogenous hormones, which is what a steroid immunoassay calibrator and a hormone-free culture condition both require. It is used as a matrix for steroid hormone calibrators and controls, as a hormone-free supplement in cell culture and in endocrinology research. Each unit contains 1 mL. MBP supplies Molecular Depot serum and plasma products to research labs in the United States, Canada and worldwide.