Name: Recombinant Canine Animal Free IL-4
Brand: Molecular Depot
SKU: B2017800
Price: 4195.00 USD
Availability: InStock

<h2 data-section-id="f727ot" data-start="0" data-end="41">What are interferons and interleukins?</h2>
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Interferons (IFN-alpha, IFN-beta, IFN-gamma) and interleukins (IL-1 through IL-18) are signaling cytokines that direct immune cell communication, antiviral response, and inflammation. Related entities include tumor necrosis factor (TNF-alpha), colony-stimulating factors (G-CSF, GM-CSF), Janus kinase (JAK) signaling, and T-cell, NK-cell, and macrophage activation assays.
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<h2 dir="ltr">What you'll find in this category: </h2>
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Antiviral Defense Modulators: Premium interferons prepared to study cellular resistance mechanisms and viral replication paths.
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Leukocyte Communication Links: Pure interleukins designed to direct immune cell activation, differentiation, and crosstalk.
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Immune Response Standards: Batch-validated signaling tools built to maintain steady, predictable baselines in co-culture models.
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<h2 data-section-id="1p9v7w8" data-start="587" data-end="632">How to choose interferons and interleukins</h2>
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Species and isoform match
Confirm the protocol calls for human, mouse, or rat recombinant protein and the correct isoform (e.g., IFN-gamma vs. IFN-alpha2a, IL-2 vs. IL-15). A mismatched species cytokine will not activate the intended receptor in most cell lines.
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Carrier protein presence
Carrier-free formats (no BSA) are preferred for applications where trace bovine protein could confound downstream assays, such as ELISA development or animal-component-free (ACF) stem cell culture. Carrier-added formats improve stability for general cell culture supplementation.
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Endotoxin level
Endotoxin-free or low-endotoxin (typically &lt;0.1 ng per microgram of protein) preparations matter for immune cell assays, since lipopolysaccharide contamination can independently activate macrophages and skew interleukin readouts.
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Activity verification (bioassay vs. SDS-PAGE)
Look for lot-specific bioactivity data (e.g., ED50 in a proliferation or reporter assay), not only purity by SDS-PAGE, when reproducibility across lots is critical for a multi-month study.
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<h2 data-section-id="1fljvas" data-start="2347" data-end="2372">Specifications context</h2>
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Concentrations for cytokine stimulation assays are typically expressed in ng/mL, with working ranges from 0.1 ng/mL to 100 ng/mL depending on the receptor's sensitivity and the readout (proliferation vs. reporter gene). Specific activity is reported in units/mg or IU/mg for interferons, following international reference standards used to calibrate antiviral potency. As of 2026, labs increasingly request carrier-free, endotoxin-tested lots when cytokines feed into single-cell or multi-omics workflows where background activation must be minimized.
For related signaling reagents, see Epidermal Growth Factors (EGF), Transforming Growth Factors, Tumor Necrosis Factors (TNF-alpha), Fibroblast Growth Factors (FGF), and the broader Growth Factors &amp; Hormones category.
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Interferons & Interleukins

Molecular Depot

Key Specifications

Recombinant Canine Animal Free IL-4

Recombinant Canine Animal Free IL-4 by Molecular Depot is a recombinant canine interleukin-4 produced without animal-derived components. IL-4 helps regulate immune responses and inflammation in dogs. It is used to differentiate canine macrophages and dendritic cells in culture, in Th2 polarisation studies and in veterinary immunology research. Each unit contains 1 mg. MBP supplies Molecular Depot research reagents to research labs in the United States, Canada and worldwide.