Name: Raf1-RBD Agarose
Brand: Molecular Depot
SKU: B2018662
Price: 2095.00 USD
Availability: InStock

<h2>What is agarose for gel electrophoresis?</h2>
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Agarose is a linear polysaccharide derived from agar that, when dissolved in hot buffer and allowed to cool, forms a porous gel matrix whose pore size is determined by the agarose concentration. DNA and RNA molecules migrate through this matrix under an electric field at a rate inversely related to their size, allowing fragments of different lengths to be separated and visualized as distinct bands.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Azura Agarose for routine electrophoresis of nucleic acids with low electroendosmosis (EEO) performance</li>
<li dir="ltr" role="presentation">Benchmark Agarose for PCR product analysis and high-quality nucleic acid separation</li>
<li dir="ltr" role="presentation">Benchmark Specialty Agarose for specialized gel applications requiring enhanced resolution and performance control</li>
<li dir="ltr" role="presentation">Standard Agarose for routine use in gel electrophoresis workflows</li>
<li dir="ltr" role="presentation">EZ Pack&trade; Agarose Tablets for agarose concentration control and convenient gel preparation&nbsp;</li>
</ul>
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<h2>How to choose agarose type and concentration</h2>
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Use standard agarose at 0.7&ndash;1% for routine PCR and restriction digest checks on larger fragments
A 0.7&ndash;1% standard agarose gel is commonly used for routine PCR product verification and restriction digest analysis when fragments are generally in the 1&ndash;10 kb size range.
Use 1.5&ndash;2% standard agarose for resolving smaller fragments in the 200 bp&ndash;2 kb range
Higher agarose concentrations from 1.5% to 2% are appropriate for resolving smaller PCR products, SNP genotyping amplicons, or restriction digests where size differences below 500 bp need to be clearly distinguished.
Use high-resolution agarose at 2&ndash;4% for very small fragments or close sizing
High-resolution agarose formulations can differentiate closely sized DNA fragments in the 50&ndash;500 bp range more effectively than standard agarose, making them useful for applications such as microsatellite or STR analysis.
Choose low-melting-point agarose when you need to recover DNA from gel slices
Low-melting-point (LMP) agarose can be melted at approximately 65&deg;C after electrophoresis, allowing DNA-containing gel slices to be processed more easily for downstream enzymatic reactions or purification workflows.
Confirm agarose purity (EEO grade) for sensitive applications
Electro-endosmosis (EEO) reflects agarose purity. Low-EEO agarose supports more consistent DNA migration and helps minimize contaminants that may interfere with downstream PCR amplification, restriction digestion, or other enzymatic applications.
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<h2>Specifications context</h2>
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Standard agarose gels are typically prepared by dissolving agarose powder in TAE or TBE buffer through heating, followed by cooling to approximately 55&deg;C before pouring into casting trays. Higher-concentration gels solidify more rapidly and generally benefit from prompt casting.
As of 2026, low-EEO agarose grades remain the preferred choice for sensitive applications such as Southern blotting, gel extraction followed by PCR, and restriction enzyme digestion workflows where contaminants can inhibit downstream reactions.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> and get premium agarose gel for your research today.

Agarose

Molecular Depot

Key Specifications

Raf1-RBD Agarose

Raf1-RBD Agarose, catalog B2018662, is the Ras-binding domain (RBD) of Raf-1 immobilized on agarose, with typical uses including Ras activation (pull-down) assays. Available from MBP, a registered vendor at leading US research institutions.