Name: Protein Sample Loading Buffer (2x, 6x)
Brand: Molecular Depot
SKU: B2014719
Price: 595.00 USD
Availability: InStock

<h2>What are electrophoresis buffers?</h2>
&nbsp;
Electrophoresis buffers are the aqueous conductive solutions that carry the electric current through the gel system and, for nucleic acid agarose gels, are also incorporated directly into the gel matrix as the dissolving buffer used to prepare the agarose. The two most widely used DNA electrophoresis buffers are TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA), which differ in buffering capacity, resolution characteristics, and downstream compatibility; MOPS is the standard buffer for RNA denaturing agarose gels; and Tris-Glycine or Bis-Tris-based systems are used for SDS-PAGE protein gel electrophoresis.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
TAE Buffer Concentrates: High-solubility Tris-Acetate-EDTA formulations optimized for effective separation of large genomic DNA fragments and efficient downstream cloning extraction.
</li>
<li dir="ltr" aria-level="1">
TBE Running Buffers: Premium Tris-Borate-EDTA solution mixes engineered for long, high-voltage gel runs and high-resolution sizing of small DNA pieces and PCR products.
</li>
<li dir="ltr" aria-level="1">
MOPS Denaturing Packs: Specialized 3-(N-morpholino)propanesulfonic acid buffer configurations calibrated for stable, high-resolution separation of RNA strands in formaldehyde matrices.
</li>
<li dir="ltr" aria-level="1">
Pre-Mixed Loading Dyes: High-density tracking formulations prepared with tracking markers to ensure uniform sample sinking and real-time migration monitoring.
</li>
</ul>
&nbsp;
<h2>How to choose electrophoresis buffers</h2>
&nbsp;
Choose TAE for routine DNA gel work and downstream gel extraction
TAE (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.3) is the most commonly used DNA electrophoresis buffer, providing good resolution for fragments larger than approximately 1 kb and allowing efficient recovery of DNA from gel slices since borate ions from TBE can interfere with some gel extraction and downstream enzymatic workflows.
Choose TBE for better resolution of small fragments and longer gel runs
TBE (90 mM Tris, 90 mM boric acid, 2 mM EDTA, pH 8.3) provides approximately 10 times higher buffering capacity than TAE and gives sharper bands for fragments below approximately 1 kb, making it better suited to long electrophoresis runs where TAE's lower buffering capacity can allow pH drift that affects resolution.
Use MOPS buffer for denaturing RNA agarose gels
MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA, pH 7.0) is the standard running buffer for formaldehyde-containing denaturing agarose gels used in RNA size analysis and northern blotting, since it maintains the pH stability needed for the denaturing conditions and is compatible with RNA detection.
Match your SDS-PAGE running buffer to your gel chemistry
Tris-Glycine running buffer (25 mM Tris, 192 mM glycine, 0.1% SDS) is the standard for conventional SDS-PAGE; Bis-Tris-based MOPS or MES running buffers are used with Bis-Tris polyacrylamide gel systems, each producing different apparent molecular weights for the same protein.
Prepare transfer buffer for western blotting from running buffer components
Western blot transfer buffer for wet or semi-dry transfer is typically prepared as Tris-Glycine base buffer with methanol added at 20% to facilitate protein release from the gel and binding to the membrane, with methanol concentration adjusted based on protein size and membrane type.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
TBE has approximately 10 times the buffering capacity of TAE because boric acid is a stronger buffer in the gel electrophoresis pH range, and this higher capacity means TBE sustains consistent electrophoresis conditions across longer runs before pH drift affects resolution. As of 2026, pre-made concentrated buffer stocks at 10X or 50X remain the most convenient format for routine use, diluted to 1X before each experiment, alongside pre-formulated SDS-PAGE running and transfer buffer options for protein gel workflows.
<a href="https://mbpinc.net/contact-us">Contact the helpful team at MBP</a> today to request a quote for your specific research requirements.

Electrophoresis Buffers

Molecular Depot

Key Specifications

Protein Sample Loading Buffer (2x, 6x)

Protein Sample Loading Buffer (2x, 6x) by Molecular Depot is a ready-to-use protein sample loading buffer supplied as 2x and 6x concentrates, containing Tris-HCl, glycerol, SDS and bromophenol blue. The glycerol makes the sample dense enough to sink into the well, the SDS denatures and charges the proteins, and the bromophenol blue tracks the migration front. It is used to prepare protein samples for SDS-PAGE and Western blotting. Each unit contains 10 mL. MBP supplies Molecular Depot electrophoresis reagents to research labs in the United States, Canada and worldwide.