Name: Protein Molecular Weight Markers, HPLC
Brand: Molecular Depot
SKU: B2021628
Price: 695.00 USD
Availability: InStock

<h2>What are protein ladders and size markers?</h2>
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Protein ladders and size markers are mixtures of purified proteins of precisely defined molecular weights, measured in kilodaltons (kDa), run in a dedicated gel lane during SDS-PAGE to provide a molecular weight scale for sizing proteins of interest in adjacent lanes. Prestained protein ladders carry colored dyes covalently coupled to each protein band, making them visible during electrophoresis to monitor protein separation progress in real time and to verify membrane transfer efficiency in western blotting. Unstained ladders provide more accurate molecular weight estimation after gel staining.
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<h2 dir="ltr">What you will find:</h2>
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<a href="https://mbpinc.net/reagents/electrophoresis/electrophoresis-reagents/protein-ladders/markers/prestained-protein-ladders">Prestained Protein Ladders: </a>including PureView&trade; Prestained Protein Ladder, Opti-Protein Marker, and Opti-Protein Ultra Marker for accurate protein size estimation and clear band identification in western blot and SDS-PAGE workflows
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<h2>How to choose protein ladders and markers</h2>
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Choose a prestained ladder to monitor SDS-PAGE and verify western blot transfer
Prestained protein ladders are visible in real time on the gel during electrophoresis, allowing you to monitor band migration and stop the run at the right time, and continue to be visible on the transfer membrane to confirm that bands have transferred completely before antibody incubation.
Use an unstained ladder when precise molecular weight determination is the priority
Unstained protein ladders give more accurate apparent molecular weight estimation because the dye used to color prestained ladders slightly alters each protein's apparent molecular weight, and the dye-free unstained version migrates more closely to the theoretical molecular weight determined by calibration against known standards.
Match your ladder's kDa range to your target protein's expected size
Choose a ladder whose coverage spans and brackets your target protein's expected molecular weight, since a protein that migrates above the largest ladder band or below the smallest cannot be accurately sized against that ladder.
Confirm ladder compatibility with your gel system and buffer chemistry
Pre-stained protein ladders have apparent molecular weights that differ depending on the SDS-PAGE buffer system used, such as Tris-Glycine versus Bis-Tris, and the gel percentage, because buffer pH affects SDS binding and protein charge, altering migration.
Use a two-color or multi-color ladder for quick gel orientation
Many prestained protein ladders include differently colored reference bands, typically used for quick visual orientation of the gel and transfer membrane.
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<h2>Specifications context</h2>
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Prestained protein ladders typically cover a range of 10 to 245 kDa depending on format, with reference bands for orientation. Ready-to-use ladders supplied in loading buffer are commonly used for routine SDS-PAGE and western blot workflows.
No more faint bands. <a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to get the right protein ladders/markers for your lab.

Protein Ladders/Markers

Molecular Depot

Key Specifications

Protein Molecular Weight Markers, HPLC

Protein Molecular Weight Markers, HPLC (B2021628) from Molecular Depot is a mixture of protein molecular weight markers for HPLC, supplied as 5 tests of lyophilized powder. HPLC molecular weight markers are used to calibrate size-exclusion columns, check column performance and estimate the native size of proteins and complexes. Available from MBP, a registered vendor at leading US research institutions.