Name: ProSpec Bio Recombinant Human Transforming Growth Factor-Beta 3 (24-412 a.a.)
Brand: ProSpec Bio
SKU: PSB-CYT-886
Availability: InStock

<h2 data-section-id="9vmnk5" data-start="0" data-end="40">What are transforming growth factors?</h2>
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Transforming growth factors include the TGF-beta superfamily isoforms (TGF-beta1, TGF-beta2, TGF-beta3) and TGF-alpha, recombinant proteins that regulate cell differentiation, immune suppression, fibrosis, and epithelial-mesenchymal transition (EMT). Related entities include SMAD signaling, fibrosis disease models, chondrogenic differentiation, regulatory T cell (Treg) induction, and EGFR signaling (for TGF-alpha).
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<h2 dir="ltr">What you'll find in this category: </h2>
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Matrix Formation Regulators: High-purity growth factors engineered to direct extracellular matrix deposition and remodeling.
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Cell Cycle Modulators: Stable proteins optimized to study cellular proliferation control and differentiation pathways.
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Morphogenesis Reference Tools: Batch-validated signaling assets prepared to guide tissue engineering and structural cell research.
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<h2 data-section-id="4el97f" data-start="651" data-end="703">How to choose transforming growth factor products</h2>
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TGF-beta isoform selection (1, 2, or 3)
TGF-beta1 is the most broadly used isoform for fibrosis models and Treg induction, TGF-beta3 is commonly specified in chondrogenic differentiation protocols for cartilage tissue engineering, and TGF-beta2 appears in some ocular and developmental biology studies. Confirm the published protocol's specified isoform rather than substituting TGF-beta1 by default.
Carrier-free vs. carrier-added formulation
Carrier-free TGF-beta is preferred for animal-component-free chondrogenic or stem cell differentiation protocols where bovine carrier protein could affect downstream matrix or proteomic analysis. Carrier-added formats provide better stability for general fibrosis or cell-line studies.
Working concentration range
TGF-beta isoforms are typically used at very low concentrations, often 1&ndash;10 ng/mL, in differentiation and fibrosis assays. Confirm the exact concentration in the published protocol, since TGF-beta is active at substantially lower doses than EGF or FGF2.
TGF-alpha vs. TGF-beta
TGF-alpha signals through the EGF receptor (EGFR) and is used in epithelial proliferation studies, distinct from the TGF-beta/SMAD pathway used in differentiation and fibrosis work. Confirm which pathway the experiment targets before ordering.
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<h2 data-section-id="1fljvas" data-start="3041" data-end="3066">Specifications context</h2>
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TGF-beta activity is reported as ED50 in a growth-inhibition or SMAD-reporter bioassay, often in the picogram-to-low-nanogram per mL range, reflecting its high potency relative to other growth factors. Pack sizes for academic labs commonly range from 2 micrograms to 50 micrograms given the low working concentrations, with purity by SDS-PAGE typically above 97%. As of 2026, chondrogenic differentiation protocols for cartilage tissue engineering continue to specify TGF-beta3 over TGF-beta1 as the isoform of choice for collagen type II expression.
This category sits within MBP's broader growth factors and hormones catalog alongside fibroblast growth factors (FGF) and epidermal growth factors (EGF), both relevant for differentiation media co-formulation. Labs studying inflammation alongside fibrosis pathways may also review tumor necrosis factors and interferons and interleukins. MBP's specialist team can confirm isoform-specific pack sizes for low-volume, high-potency reagents like TGF-beta before order placement.
<a href="https://mbpinc.net/contact-us">Message the helpful MBP team</a> directly to discover the ideal growth factor configurations for your specific workflows.

Transforming Growth Factors

ProSpec Bio

Key Specifications

ProSpec Bio Recombinant Human Transforming Growth Factor-Beta 3 (24-412 a.a.)

Recombinant Human Transforming Growth Factor-Beta 3 (24-412 a.a.) by ProSpec Bio is human transforming growth factor-beta 3 (TGF-beta3) (amino acids 24-412) produced in E. coli with a 23-amino-acid His tag at the N-terminus, a 412-amino-acid protein of 47.2 kDa that is more than 85% pure. It is supplied as a solution (0.5 mg/mL in 20 mM Tris-HCl (pH 8.0) and 10% glycerol) in 5 µg, 20 µg and 1 mg sizes for TGF-beta and chondrogenesis research.