Name: ProSpec Bio Recombinant Human Neurotrophin-3
Brand: ProSpec Bio
SKU: PSB-CYT-257
Availability: InStock

<h2 data-section-id="1osgbn" data-start="0" data-end="33">What are nerve growth factors?</h2>
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Nerve growth factor (NGF), most commonly beta-NGF (2.5S NGF in mouse-derived nomenclature), is a recombinant neurotrophin that binds the TrkA receptor and the p75 neurotrophin receptor (p75NTR) to promote neuronal survival, differentiation, and neurite outgrowth. Related entities include PC12 cell differentiation, dorsal root ganglion (DRG) explant culture, sympathetic neuron survival assays, brain-derived neurotrophic factor (BDNF) and neurotrophin family signaling, and TrkA/p75NTR receptor pathways.
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<h2 dir="ltr">What you'll find in this category: </h2>
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Neurotrophic Signaling Links: Highly purified proteins prepared to support specific neuronal growth pathways.
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Axonal Outgrowth Assets: Stable growth factors designed to induce and measure neurite branching in vitro.
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Neural Culture Standards: Batch-validated molecular tools optimized to maintain delicate primary neural cell populations.
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<h2 data-section-id="1aa4gck" data-start="721" data-end="750">How to choose NGF products</h2>
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Species match (human vs. mouse)
Recombinant human beta-NGF and mouse 2.5S NGF are both available. Mouse NGF is historically the most-cited form in classic PC12 differentiation literature, while human recombinant NGF is specified in protocols using human-derived neuronal models. Confirm the published protocol's species before ordering.
Carrier-free vs. carrier-added formulation
Carrier-free NGF (no BSA) is preferred for neurite outgrowth assays where carrier protein could interfere with imaging-based readouts or downstream proteomics. Carrier-added NGF provides improved stability for longer-term neuronal culture feeding schedules.
Concentration and assay type
PC12 cell differentiation protocols commonly use NGF at 50&ndash;100 ng/mL, while lower concentrations (1&ndash;10 ng/mL) may be used in survival assays for primary sensory or sympathetic neurons. Confirm the specific assay's published concentration before scaling an order.
Activity verification
Look for lot-specific bioactivity data from a neurite outgrowth or PC12 differentiation bioassay rather than purity alone, since NGF activity can vary between lots even at comparable SDS-PAGE purity.
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<h2 data-section-id="1fljvas" data-start="2818" data-end="2843">Specifications context</h2>
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NGF activity is reported as ED50 in a neurite outgrowth or PC12 differentiation bioassay, generally in the low ng/mL range, with purity by SDS-PAGE commonly above 95&ndash;98%. Pack sizes for academic neuroscience laboratories typically range from 10 micrograms to 100 micrograms because most differentiation protocols use relatively low working concentrations. As of 2026, neurite outgrowth assays using carrier-free NGF combined with high-content imaging remain a standard approach for quantifying neurotrophin response in both primary and iPSC-derived neuronal cultures.
This category is part of MBP's growth factors and hormones catalog alongside insulin-like growth factors, which also support neural progenitor signaling in some protocols, and fibroblast growth factors (FGF), which are commonly used in neural stem cell maintenance. Laboratories running combination neurotrophin protocols may also explore peptide hormones for related signaling reagents. MBP's specialist team can help source NGF combined with BDNF or NT-3 for multi-neurotrophin survival assays.
<a href="https://mbpinc.net/contact-us">Message the helpful MBP team</a> directly to find the ideal neurotrophic factor configurations for your neural runs.

Nerve Growth Factors

ProSpec Bio

Key Specifications

ProSpec Bio Recombinant Human Neurotrophin-3

Recombinant Human Neurotrophin-3 by ProSpec Bio is tag-free human neurotrophin-3 (NT-3) produced in E. coli as a non-covalent homodimer of two 120-amino-acid chains of 27.5 kDa, more than 95% pure. It shows an ED50 of 3.49 ng/mL in a neuroblastoma proliferation assay. It is supplied lyophilized in 2 µg, 10 µg and 1 mg sizes for neuronal culture and neurotrophin research.