Name: ZymoBIOMICS MagBead DNA/RNA
SKU: R2135
Availability: InStock

<h2>What are Magnetic Bead Microbial DNA/RNA Co-Extraction Kits?</h2>
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Magnetic bead microbial DNA/RNA co-extraction kits purify both genomic DNA and total RNA, including small RNAs &gt;=17 nt, from microbial community samples - stool, soil, water, biofilm, swabs, and body fluids using BashingBead mechanical lysis in a 96-deep-well plate followed by binding of nucleic acids to paramagnetic silica-coated beads, magnetic separation during successive wash steps to remove PCR inhibitors, and elution in &gt;=50 ul of ZymoBIOMICS DNase/RNase-Free Water. The entire purification phase requires no centrifugation and integrates directly with KingFisher Flex, Accuris IsoPure 96, and AllSheng AutoPure 96 liquid-handling platforms. Choose the magnetic bead format for microbial DNA/RNA co-extraction when processing 48+ community samples per session, when a compatible automation platform is available, or when reproducibility across hundreds of samples is required for a human microbiome cohort, environmental monitoring, or clinical trial.
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<h2 dir="ltr">What you will find:</h2>
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Quick-DNA/RNA HT Kit for high-throughput isolation of total nucleic acids from liquid clinical and biological specimens using a magnetic bead&ndash;based extraction system.
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZymoBIOMICS MagBead DNA/RNA Kit for unbiased recovery of microbiome DNA and RNA without chemical bias, featuring an innovative lysis system.
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA Pathogen MagBead Kit for rapid isolation of viral, bacterial, and protozoan nucleic acids from challenging vector samples using an optimized buffer
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<h2>How to Choose a Magnetic Bead Microbial DNA/RNA Co-Extraction Kit</h2>
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Automation Script Availability
Confirm the kit manufacturer provides a validated, instrument-specific script for your platform before purchase. Zymo Research provides automation scripts for KingFisher Flex, KingFisher Apex, Accuris IsoPure 96, and AllSheng AutoPure 96; Tecan Fluent and Hamilton STAR scripts require adaptation. Script development and validation add 2-4 weeks; factor this into project timelines.
Combined vs. Separate Analyte Fractions
The ZymoBIOMICS MagBead DNA/RNA kit elutes a combined total nucleic acid fraction. For studies requiring separate DNA and RNA fractions from the same extraction (parallel metagenomics and metatranscriptomics), apply post-extraction enzymatic depletion (DNase I on the RNA aliquot, RNase A on the DNA aliquot) or use the ZymoBIOMICS DNA/RNA Miniprep kit, which uses column-switching architecture for native separation.
96-Well Bead-Beating Setup
The lysis step requires a 96-well bead mill compatible with ZR-96 BashingBead Lysis Racks. Validated instruments include GenoGrinder 2000, Retsch MM400 with a 96-tube rack adapter, and Bertin Precellys. The bead-beating step is the primary manual step in an otherwise automated workflow; confirm the lab has a compatible bead mill before adopting the kit.
Inhibitor Load by Sample Type
Stool samples have moderate inhibitor loads (bile salts, starch metabolites) that ZymoBIOMICS wash steps reliably remove. Soil samples -- especially high-humic tropical or peat soils - carry heavier inhibitor loads that may require reducing input mass to 10-50 mg per well and validating A260/A230 &gt;= 1.5 on initial test extractions. If A260/A230 remains below 1.5, apply an additional cleanup step with Zymo OneStep PCR Inhibitor Removal Columns before library preparation.
DNA/RNA Shield Sample Collection
For multi-site human microbiome cohort studies, use DNA/RNA Shield fecal collection tubes (Zymo R1101) as the collection device. Samples stabilized at room temperature for up to 30 days in Shield are directly loadable into the ZR-96 BashingBead Rack, streamlining the pre-extraction workflow and enabling consistent RNA preservation across diverse collection sites.
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<h2>Specifications Context</h2>
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ZymoBIOMICS MagBead DNA/RNA kit produces total nucleic acid eluted into &gt;=50 ul with A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and DNA fragment sizes suitable for 16S rRNA amplicon sequencing and shotgun metagenomics. Input specification: approximately 2 &times; 10^8 bacterial cells, 2 &times; 10^7 yeast cells, or ~40 mg human stool per well. A published 2024 high-throughput fecal microbiome DNA extraction study demonstrated that bead-beating at &gt;=3,000xg significantly improves detection of hard-to-lyse Gram-positive genera - Blautia, Bifidobacterium, and Ruminococcus compared with no bead-beating, confirming that mechanical lysis is non-negotiable for unbiased community co-extraction. ZymoBIOMICS MagBead workflows are used in NIH-funded human microbiome research and are the standard extraction approach for multi-site gut microbiome cohort studies at institutions registered with MBP, including Howard Hughes Medical Institute and MD Anderson Cancer Center.
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<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> and get high-yield mag bead systems for your high-throughput extraction workflows.

Mag Beads

MSDS

DataSheet

Protocol

ZymoBIOMICS MagBead DNA/RNA

R2135 by Zymo Research is the ZymoBIOMICS&#8482; MagBead DNA/RNA kit for simultaneous co-purification of DNA and total RNA from complex microbiome samples in a 96-well magnetic bead format. With a binding capacity of 15 µg DNA/RNA per 30 µl beads and elution in 50 µl, R2135 delivers NGS-ready nucleic acids (A260/A280 &gt;1.8) from fecal, soil, water, biofilm, and swab samples, with compatibility for automated liquid handling platforms and robotic processors.