The D404 Zymo ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spinâ„¢ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 10 µl |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0 |
| Size Range | Up to ~200 kb |
| Yield | Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture. |
Q1: I accidently left my P3 Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
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