The D404 Zymo ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
|Applicable For||Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.|
|Elution Volume||≥ 10 µl|
|Processing Time||15 min|
|Purity||Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0|
|Size Range||Up to ~200 kb|
|Yield||Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.|
Q1: I accidently left my P3 Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.