Name: Ultra-Pure BlasTaq™ 2X PCR MasterMix
SKU: G885
Price: 180.57 USD
Availability: InStock

<h2 dir="ltr">What Is Hot Start Taq DNA Polymerase?</h2>
 Hot-start Taq DNA polymerase is the enzyme of choice for PCR applications where non-specific amplification or primer dimer formation has been problematic with standard Taq. By keeping the polymerase inactive until a high-temperature activation step, hot-start formulations prevent the room-temperature primer extension that drives non-specific products in standard Taq reactions. Choose a polymerase suited to your assay requirements.
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<h2 dir="ltr">What you will find:</h2>
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<li data-section-id="1hsv5i3" data-start="233" data-end="391">Hot-start Taq DNA polymerases are designed to support multiplex PCR by reducing non-specific amplification and primer-primer interactions during reaction setup.</li>
<li data-section-id="bhgh5r" data-start="392" data-end="516">Formulations suitable for high-throughput PCR workflows requiring a stable room-temperature setup before thermal activation.</li>
<li data-section-id="1h2z623" data-start="517" data-end="640">Improved specificity for amplification of low-abundance targets compared with conventional non-hot-start Taq polymerases.</li>
<li data-section-id="1nifv8r" data-start="641" data-end="743">Cleaner amplicon generation for downstream applications such as cloning, sequencing, and genotyping.</li>
<li data-section-id="bof93a" data-start="744" data-end="892">Enzyme and buffer systems formulated to support amplification of challenging templates, including GC-rich regions and complex genomic DNA samples.</li>
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<h2 dir="ltr"> How to Choose Hot Start Taq DNA Polymerase</h2>
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Inactivation Mechanism Antibody-mediated formulations use a heat-sensitive antibody that reversibly binds and inhibits the enzyme until denatured during the initial high-temperature step. Chemically modified formulations use covalent modifications to active-site residues that are reversed by heat during cycling. Standard vs. Hot-Start Comparison Standard Taq DNA polymerase is a formulation of recombinant Taq that remains active at ambient temperatures, permitting non-specific primer extension during reaction setup &mdash; hot-start formulations are reversibly inactivated at ambient temperature specifically to prevent this. Buffer Compatibility As with standard Taq, optimised 10x PCR buffer formulations are designed to complement the specific hot-start enzyme's activation and activity profile. Activation Time Considerations Confirm whether your chosen hot-start formulation activates within a standard initial denaturation step or requires an extended/dedicated activation incubation, as this affects total cycling protocol time. Application Fit Hot-start Taq is recommended wherever specificity is a concern: multiplex PCR, low-template-abundance amplification, diagnostic PCR, and any workflow where primer dimer formation has been observed with standard Taq. 
<h2 dir="ltr"> Specifications Context</h2>
 Hot-start Taq DNA polymerase is a recombinant Taq formulation that remains inactive during reaction setup, preventing the non-specific primer extension that standard Taq can permit before thermal cycling begins. This improved specificity is particularly valuable for multiplex PCR, low-abundance templates, and applications where non-specific products could affect result interpretation. Hot-start enzymes are typically stored at &minus;20 &deg;C and commonly supplied with matched PCR buffer. Because antibody-mediated and chemically modified formats can require different activation conditions, initial denaturation parameters should be verified when introducing a new enzyme. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote and identify the most suitable hot start Taq DNA polymerase for your workflow.

Hot Start Taq DNA Polymerase

DataSheet

MSDS

PCR Product Guide

Ultra-Pure BlasTaq™ 2X PCR MasterMix

G885 Ultra-Pure BlasTaq&trade; 2X PCR MasterMix by Applied Biological Materials is an ultra-pure, human DNA-free 2X fast PCR master mix with BlasTaq&trade; DNA Polymerase (6 kb/min; 40&ndash;70% faster than Taq) and integrated gel loading dye, for sensitive PCR applications requiring contamination-free reagents in forensic, clinical, metagenomic, and food safety workflows.