Name: TruFi™ II Ultra 2x PCR Mix
SKU: AZ-1920
Availability: InStock

High-Fidelity Master Mix

<h2 dir="ltr">What Is a High-Fidelity PCR Master Mix?</h2>
 A high-fidelity PCR master mix is a 2x concentrated, pre-formulated solution containing a proofreading DNA polymerase (Phusion, Q5, KOD, or Pfu-equivalent), dNTPs, MgCl2, and reaction buffer &mdash; requiring only the addition of template and primers to set up a reaction. The proofreading polymerase's 3' to 5' exonuclease activity corrects misincorporated nucleotides during synthesis, reducing the error rate roughly 50-100-fold compared to standard Taq master mixes. Choose a high-fidelity master mix whenever the amplicon will be cloned, mutagenised, or sequenced &mdash; not for routine genotyping, where standard Taq is sufficient and lower cost.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="uyjmeg" data-start="418" data-end="539">Streamlined post-amplification analysis with high-fidelity 2X red mix formulations with direct gel-loading capability</li>
<li data-section-id="1rh0fvx" data-start="540" data-end="630">Pre-optimized high-fidelity 2X PCR master mixes containing proofreading DNA polymerase</li>
<li data-section-id="6drhog" data-start="631" data-end="735">Reduced non-specific amplification and primer-dimer formation compared to standard Taq-based systems</li>
<li data-section-id="1pa7u4r" data-start="736" data-end="826">High-fidelity amplification suitable for cloning workflows requiring sequence accuracy</li>
<li data-section-id="1n3ow6j" data-start="827" data-end="925">2X master mix formats designed for reproducible amplification across a range of template types</li>
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<h2 dir="ltr"> How to Choose a High-Fidelity Master Mix</h2>
 Downstream Application Cloning into expression vectors, site-directed mutagenesis, and sequencing-bound amplicons all benefit from the reduced error rate of a high-fidelity master mix. Routine genotyping or colony screening does not require this level of accuracy and is typically served by standard Taq master mixes at a lower cost. Template Difficulty (GC Content, Length) Some high-fidelity master mix formulations are specifically optimised for GC-rich templates or long amplicons (&gt;5 kb), where standard high-fidelity formulations may show reduced yield. Check product-specific template compatibility notes before ordering for difficult targets. Extension Time and Cycling Protocol High-fidelity polymerases often have different optimal extension times per kilobase compared to standard Taq &mdash; some extend faster, shortening overall cycling time for long amplicons. Follow the manufacturer's recommended cycling parameters rather than reusing a standard Taq protocol. Cloning Method Compatibility Some high-fidelity master mixes produce blunt-ended products suited to Gibson Assembly or blunt cloning strategies; others may need additional A-tailing for TA cloning. Confirm compatibility with your intended downstream cloning method before selecting a product. Format Convenience vs. Component Flexibility The 2x master mix format minimises pipetting steps and improves reproducibility for routine high-fidelity workflows. Researchers needing to titrate individual components (e.g., additional MgCl2 for a specific template) may prefer the individual high-fidelity enzyme plus separate buffer instead. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x high-fidelity master mix is set up identically to a standard 2x Taq master mix &mdash; combined with template and primers at the manufacturer-specified ratio to reach 1x working concentration &mdash; but the included proofreading polymerase detects and removes misincorporated bases immediately after insertion, reducing the per-cycle error rate to approximately 10⁻⁶ per nucleotide. This level of accuracy matters specifically because any error introduced during amplification is propagated into the final cloned construct or sequencing read. High-fidelity master mixes are typically stored at &minus;20 &deg;C; lot-specific CoAs documenting activity and quality control are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get pre-optimized High-Fidelity Master Mix solutions for your lab.

User Manual

Product Literature

TruFi™ II Ultra 2x PCR Mix

TruFi II Ultra 2x PCR Mix by Azura Genomics — &gt;75× fidelity vs Taq; 10 sec/kb extension; &gt;15 kb long PCR; 2× with buffer, MgCl₂, and dNTPs included. Available in 100 (AZ-1920), 500 (AZ-1921), and 1000 (AZ-1922) reaction formats.