



T5997 Enhanced Primary Human Liver Sinusoidal Endothelial Cells by ABM — expanded LSECs; MMR+/CD31+; 5×10⁵ cells/1.0 ml, BSL-II. Seeding 10,000 cells/cm²; max 1–2 passages; subculture at 70–80%. TrypLE Express (PBS wash first); 280×g; TM111 thaw → TM112 growth media; G422 coating. MTA required.