
T5549 Normal Human Primary Hepatic Kupffer Cells by ABM — resident liver macrophages expressing CD68, TNF-α, IL-6, CD34, F4/80; 5×10⁵ cells/1.0 ml, BSL-II. Seeding 13,000–15,000 cells/cm²; 100×g centrifuge; 48h undisturbed recovery; max 7-day culture; no subculture. G999 ECM flasks and PriGrow TM5549 required. MTA required.