
T5421 Human Primary Kidney Glomerular Endothelial Cells by ABM — CD31/VE-Cadherin positive; cobblestone; 5×10⁵ cells/1.0 ml, BSL-II. Unique protocols: 120×g thaw; 0.05% trypsin subculture; do NOT centrifuge post-trypsin; 1:2 split; daily media when >70% confluent. Gelatin-coated G299 + TM104 required. MTA required.