
T4182 Human Primary Trabecular Meshwork Cells are IOP-regulating ocular cells for POAG pathobiology, aqueous humor outflow, anti-glaucoma drug discovery, and TM ECM remodeling research. Critical protocol differences: Poly-L-lysine flasks; do NOT centrifuge at thawing; 0.05% trypsin:PBS (1:1) RT; 1000 rpm centrifugation; up to 12 passages; PDT 36h. Supplied by ABM as 5×105 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.