Name: RNA Clean & Concentrator™-25
SKU: R1017
Availability: InStock

<h2>What are RNA Cleanup Kits?</h2>
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RNA cleanup kits purify and concentrate pre-extracted or synthesized RNA by removing enzymes, buffer components, unincorporated NTPs, DNA contamination, organic solvents, and environmental inhibitors (humic acids, polyphenols) from RNA solutions using silica-membrane spin columns with a simple bind-wash-elute protocol in under 10 minutes. Cleanup kits are the mandatory intermediate step between primary RNA extraction or enzymatic RNA synthesis and sensitive downstream applications - including rRNA depletion, NGS library preparation, in vitro translation, and mRNA vaccine formulation - where residual enzymes or inhibitors would cause assay failure. Zymo RNA Clean &amp; Concentrator kits elute RNA in as little as 6 ul, concentrating inhibitor-free RNA from volumes up to 500 ul into a minimal volume suitable for low-input sequencing library preparation. Use an RNA cleanup kit after any enzymatic reaction that generates RNA (DNase I treatment, IVT, capping, polyadenylation) and after environmental RNA extraction when A260/A230 is below 1.5 - never skip this step and proceed directly to library preparation or rRNA depletion.
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<h2 dir="ltr">What you will find:</h2>
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RNA Clean &amp; Concentrator&trade; (RCC): Effective spin-column systems (5, 25, 100 preps) created to concentrate low RNA concentrations into ultra-pure volumes while eliminating dyes and inhibitors.
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ZR-96 RNA Clean &amp; Concentrator&trade;: 96-well plate formats that are scalable and tailored for high-throughput lab settings while maintaining genomic integrity.
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Magbead RNA Cleanup: Sophisticated magnetic bead technology designed for automated processes, guaranteeing reproducibility-verified outcomes and uniform recovery of total RNA.
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Small RNA Retrieval: Refined methods that enhance the preservation of microRNA and various small RNA types, crucial for precise isoform identification and bioinformatics evaluation.
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<h2>How to Choose an RNA Cleanup Kit</h2>
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Binding Capacity (Format Selection)
Match the kit format to your expected RNA input mass to avoid overloading: Zymo RCC-5 (&lt;=10 ug, elute &gt;=6 ul) for routine RT-qPCR and small-batch RNA-seq cleanup; RCC-25 (&lt;=50 ug, elute &gt;=25 ul) for standard IVT and RNA-seq preparations; RCC-100 (&lt;=100 ug, elute &gt;=30 ul) for large-scale IVT or mRNA synthesis; NEB Monarch 500 ug format for high-yield preparative IVT in mRNA vaccine development. Overloading reduces yield nonlinearly; test a small input aliquot first if the expected yield is uncertain.
DNase I-Included vs. DNase-Free Formats
Zymo RCC kits catalog numbers R1013 and R1014 include DNase I for gDNA removal during cleanup - combining the DNase treatment and concentration into a single column workflow. For RNA samples where gDNA contamination is confirmed (co-extracted total RNA, environmental RNA), use the DNase I-included format. For IVT RNA (no genomic DNA present) or post-DNase RNA that only needs cleanup, use the standard RCC format without DNase.
Size-Selective Recovery
Adjust ethanol ratio in the binding step for size-selective cleanup: total RNA (&gt;=17 nt) uses a high ethanol ratio; large RNA-only (&gt;200 nt) uses a lower ratio; small RNA (17-200 nt) is collected from the flow-through of the large RNA step and rebound on a second column at high ethanol. This two-step selection from one sample enables preparation of paired large RNA and small RNA fractions for simultaneous mRNA-seq and miRNA-seq library preparation without separate primary extraction.
Environmental RNA Inhibitor Removal
For soil or fecal RNA with A260/A230 below 1.5, RCC-5 cleanup consistently raises A260/A230 to &gt;1.8 by binding RNA and washing away humic acids, polyphenolics, and fulvic acids. Run the cleanup before proceeding to rRNA depletion - inhibitor-contaminated RNA causes inefficient depletion, wasting expensive reagents. Add an RNA quantification step (Qubit RNA HS Assay) before and after cleanup to confirm both recovery and inhibitor removal.
mRNA vs. Total RNA
For mRNA-specific applications (mRNA vaccine, mRNA for in vitro translation), cleanup removes abortive transcripts, DNA template, and IVT enzymes from the desired full-length mRNA. For total RNA from cells or tissue (containing rRNA, mRNA, tRNA, and small RNA), the cleanup removes extraction reagent contamination before rRNA depletion or library preparation.
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<h2>Specifications Context</h2>
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Zymo RNA Clean &amp; Concentrator kits use a single-buffer system with Zymo-Spin IC column technology, recovering total RNA &gt;=17 nt, large RNA &gt;200 nt, or small RNA 17-200 nt by adjusting ethanol conditions. RNA is eluted in as little as 6 ul of RNase-free water, ready for RT-qPCR, RNA-seq, hybridization, and sequencing without further processing. The kits remove enzymes, NTPs, unincorporated nucleotides, humic acid, polyphenolics, dyes, salts, and organic solvents from RNA solutions. RNA cleanup is a standard workflow step in metatranscriptomics (before rRNA depletion from environmental RNA), mRNA vaccine development (IVT product cleanup before formulation), and RNA-seq library preparation (cleanup after DNase I treatment) at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
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Ensure your RNA quality and eliminate interference&mdash;<a href="https://mbpinc.net/contact-us">get in touch with MBP Inc.</a> now to obtain a quote for your RNA purification reagents.

RNA Cleanup Kits

MSDS

DataSheet

Protocol

RNA Clean & Concentrator™-25

R1017 by Zymo Research is the RNA Clean &amp; Concentrator™-25 (50 preps), a 5-minute spin-column kit that recovers &gt;90% of total RNA ≥17 nt from enzymatic reactions, DNase-treated samples, IVT products, and TRIzol extracts, with elution in as little as 25 µL for NGS, RT-qPCR, and microarray applications.

RNA Clean & Concentrator™-25

SKU : R1017