Name: pRetroG-CMV-GFP-IRES-GFP Vector
SKU: RV004
Price: 120.65 USD
Availability: InStock

<h2 dir="ltr"> What Are Retroviral Control &amp; Reporter Constructs?</h2>
 Every retroviral over-expression experiment requires a matched set of control constructs to confirm that transduction was successful, the packaging system produced infectious virus at an adequate titer, and the empty vector backbone does not itself alter the measured phenotype. Retroviral control and reporter vectors use the same LTR and backbone configuration as experimental over-expression vectors. Reliable controls and reporters improve confidence in transduction and expression studies. Select the control/reporter system appropriate for your experiment.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="w0hi5b" data-start="4179" data-end="4269">Retro-CMV Blank Retroviruses used as baseline controls for retroviral delivery systems</li>
<li data-section-id="ut4vdp" data-start="4270" data-end="4370">Fluorescent Reporter Retroviruses (GFP, RFP) for real-time monitoring of transduction efficiency</li>
<li data-section-id="izipmv" data-start="4371" data-end="4456">Bicistronic GFP Reporter Vectors for co-expression and experimental normalization</li>
<li data-section-id="we28ta" data-start="4457" data-end="4544">Epitope-tagged Control Vectors supporting protein detection and expression analysis</li>
<li data-section-id="47lirb" data-start="4545" data-end="4642">Stop-codon Reporter Plasmids for assessing baseline expression and vector background activity</li>
<li data-section-id="1d2i69z" data-start="4643" data-end="4735">CMV-driven control plasmids used in retroviral expression and stable cell line workflows</li>
</ul>
<h2 dir="ltr"> How to Choose Retroviral Control &amp; Reporter Constructs</h2>
 Empty Vector Backbone Matching The empty vector control must match the experimental vector in backbone, LTR configuration, internal promoter, and selection marker &mdash; for pBABE experiments, pBABE-puro empty is correct; for pLNCX2 experiments, an empty pLNCX2 vector is required. Mismatched controls introduce confounding variables. Transduction Efficiency Verification GFP-expressing retroviral controls (pBABE-GFP, pMSCV-GFP) enable real-time FACS-based quantification before proceeding with experimental constructs &mdash; Adequate efficiency is typically assessed empirically based on cell type and experimental design, ensuring that packaging and infection conditions are suitable for downstream applications. Histochemical Detection Needs pLAPSN, derived from the pLXSN backbone, expresses bacterial alkaline phosphatase (AP), detectable histochemically with substrates such as NBT/BCIP &mdash; useful when fluorescence-based detection is not available or appropriate, particularly in fixed tissue sections. In Vivo Tracking Requirements Retroviral GFP and luciferase reporter cell lines support in vivo tracking of transplanted cells in mouse models, including haematopoietic reconstitution studies and tumour xenograft experiments via bioluminescence imaging. Multiplexing Considerations Retroviral GFP reporters are compatible with red-channel markers (RFP, mCherry, tdTomato) in multiplexed imaging and FACS &mdash; ensure control and experimental constructs use spectrally distinct reporters. 
<h2> Specifications Context</h2>
 Retroviral control and reporter systems using Retro-CMV and pRetroG-CMV vectors deliver CMV-driven expression cassettes packaged as replication-incompetent retroviral particles for transduction of actively dividing mammalian cells. These systems support stable genomic integration of GFP/RFP reporters, HA-tagged constructs, bicistronic IRES-based expression formats, and empty vector controls for baseline comparison and experimental normalization. Following transduction, integrated expression is maintained through cell division under selection or reporter-based enrichment, enabling consistent gene expression analysis, functional studies, and stable cell line generation in retroviral workflows. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get advanced retroviral control/reporters for your lab today.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

pRetroG-CMV-GFP-IRES-GFP Vector

RV004 by Applied Biological Materials is a CMV-promoter retroviral transfer vector (1.0 µg) with a bicistronic GFP-IRES-GFP expression cassette. The dual GFP design provides a strong fluorescence transduction control and validates IRES-mediated internal translation in dividing mammalian cells. Package with Retro-Easy™ (E-513) and use ViralEntry™ (G515) to maximize transduction efficiency. Store at &minus;20°C.