Name: pLenti-III-mir-GFP Control Vector
SKU: m001
Price: 80.72 USD
Availability: InStock

<h2 dir="ltr">What Are miRNA Lentiviral Negative Controls?</h2>
 MicroRNAs (miRNAs) are approximately 22-nucleotide non-coding RNAs that regulate gene expression post-transcriptionally by binding to the 3&prime; UTR of target mRNAs, triggering mRNA degradation or translational repression via the RNA-induced silencing complex (RISC). Lentiviral delivery of miRNA expression constructs enables stable, long-term modulation of miRNA activity &mdash; essential where transient transfection approaches are insufficient.
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<h2 dir="ltr">What you will find:</h2>
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Control Viruses models used in miRNA overexpression studies, for negative control normalization
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Validation of 3'UTR target utilizing bioluminescent-based reporter assays.
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miRNA-Off Control Lentiviruses used to baseline microRNA knockdown effects.
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Blank 5&rsquo; UTR reporter vector optimized for gene expression normalization and translational regulation studies&nbsp;
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Reporter Stable Cell Lines models with Luciferase or GFP markers for reliable assay standardization.
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<h2 dir="ltr"> How to Choose a miRNA Negative Control</h2>
 Experiment Type Matching miRNA mimic (gain-of-function) experiments require a non-targeting miRNA mimic control. miRNA inhibitor/antagomiR (loss-of-function) experiments require a non-targeting inhibitor control. Lentiviral miRNA expression requires an empty vector or scrambled miRNA sequence. Mimic Control Design A non-targeting miRNA mimic control is a double-stranded RNA molecule resembling a mature miRNA duplex but with no significant homology to any known miRNA target in human, mouse, or rat transcriptomes &mdash; entering RISC the same way as a functional mimic. Inhibitor Control Design Non-targeting miRNA inhibitor (antagomiR) controls are single-stranded antisense sequences that enter the relevant cellular compartment without blocking any known miRNA, establishing the baseline for inhibitor-treated versus non-inhibitor comparisons. Vector Backbone for Lentiviral Delivery Stable lentiviral expression of miRNAs is used for long-term gain-of-function studies; the matched non-targeting lentiviral control must use the same promoter and vector backbone as the experimental construct to isolate sequence-specific effects. Inducible Format Availability Tet-On (doxycycline-responsive) non-targeting miRNA lentiviral controls are available for experiments requiring temporal control, used alongside inducible experimental constructs to control for doxycycline treatment itself. 
<h2 dir="ltr"> Specifications Context</h2>
 miRNA controls are designed to reflect the endogenous processing pathway of microRNAs, including Drosha and Dicer-mediated maturation before RISC loading. Because siRNA and miRNA constructs enter the RNA interference pathway differently, pathway-matched controls are important for accurate experimental interpretation. Non-targeting miRNA controls help distinguish sequence-specific effects from background effects associated with vector delivery and miRNA pathway activation. Available formats include lentiviral vectors, packaged viruses, reporter constructs, and stable cell line controls for miRNA and UTR-based studies. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and request a quote for efficient miRNA control requirements.

miRNA Control

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

Lentivector Packaging Protocol

Lentivirus Infection Protocol

pLenti-III-mir-GFP Control Vector

m001 by Applied Biological Materials is the insert-empty pLenti-III-mir-GFP control vector (500 ng) — the matched negative control plasmid for miRNA overexpression experiments using the pLenti-III-mir-GFP (m016) backbone. Retains the GFP reporter and miRNA scaffold with no insert. Package with a third-generation lentiviral system or transfect directly. Store at &minus;20°C.