
LB Agar G247 — 500 g ready-to-autoclave powder (35–40 g/L, 121 °C 15 min). Non-selective rich agar medium for E. coli colony growth. Add antibiotic supplements at 55 °C before pouring plates (~25 ml per 90 mm dish). For transformation plating, antibiotic selection, and blue/white screening. Store cool and dry.