Name: AAV Packaging Mix (Serotype 4)
SKU: AAV1004
Price: 370.64 USD
Availability: InStock

<h2 dir="ltr">What Are AAV Purification &amp; Packaging Kits?</h2>
 Producing high-titer, high-purity recombinant AAV is a prerequisite for reproducible in vitro and in vivo gene delivery experiments. Impure AAV preparations containing cell debris, empty capsids, and residual transfection reagents reduce functional titer, increase immune activation, and compromise experimental reproducibility. Consistent AAV production requires efficient packaging and purification. Select the kit designed for your vector workflow.
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<h2 dir="ltr">What you will find:</h2>
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AAV Packaging Mix(1-6) for serotype-specific and efficient gene delivery and AAV production&nbsp;
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AAV Serotype Blast&trade; Kit for greater viral yield and enhanced AAV production workflows
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AAViralEntry&trade; Transduction Enhancer for improved uptake of AAV and better delivery in mammalian cells
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AAV Serotype Blast&trade; Kit for reliable viral vector constructs and AAV production
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<h2 dir="ltr"> How to Choose AAV Purification &amp; Packaging Kits</h2>
 Helper-Free Packaging Components Helper-free triple-transfection requires the transgene plasmid (gene of interest flanked by AAV2 ITRs), the rep/cap plasmid (supplying Rep replication proteins and the serotype-specific capsid), and a helper plasmid supplying adenoviral helper functions &mdash; confirm all three components are available for your chosen serotype. Purification Method Iodixanol gradient purification separates full and empty capsids by density without ultracentrifugation in some protocols, while precipitation-filtration kits offer a simpler benchtop alternative for labs without gradient centrifugation capability. Empty vs. Full Capsid Ratio Empty AAV capsids (lacking packaged genome) contribute to particle count without functional activity and can increase immune response in vivo &mdash; purification methods that enrich for full capsids improve both potency and safety profile. Serotype-Specific Rep/Cap Plasmids Each AAV serotype requires its corresponding rep/cap plasmid; confirm the packaging kit includes (or is compatible with) the rep/cap plasmid for your target serotype. Scale of Production Kit formats differ for small-scale research production versus larger-scale preparations intended for multiple in vivo cohorts &mdash; match kit scale to your experimental throughput needs. 
<h2 dir="ltr"> Specifications Context</h2>
 Helper-free triple transfection eliminates the need for co-infection with a replication-competent helper adenovirus, improving safety and simplifying production for standard BSL-2 laboratories. Iodixanol gradient and precipitation-filtration purification approaches are designed to produce research-grade rAAV without requiring ultra-centrifuge access, broadening accessibility for labs setting up AAV production for the first time. As with all recombinant AAV work, production requires institutional biosafety committee (IBC) approval at BSL-2 containment in most jurisdictions. Labs producing AAV for the first time should also plan for functional titering after purification by qPCR for genome copies and by transduction assay for infectious titer, since these two measures can differ substantially, and the functional titer is the more relevant figure for planning experimental MOIs. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP to confirm packaging system compatibility and serotype availability for your application.

Purification & Packaging Kits

Purfication & Packaging Kits

DataSheet

AAV Packaging Protocol

AAV Packaging Mix (Serotype 4)

AAV1004 by Applied Biological Materials is a 400 µL second-generation AAV serotype 4 packaging plasmid mix (pAAV-Helper + pAAV-R2-C4). Add transfer plasmid + co-transfect into HEK293T for rAAV4 production. AAV4 tropism includes ependymal and choroid plexus cells. Store at −20°C or below.