Name: AAV-CMV-DIO-Blank-SV40-GFP Vector
SKU: AAV104
Price: 258.91 USD
Availability: InStock

<h2 dir="ltr">What Are AAV DIO/FLEX Vectors?</h2>
 The DIO (Double-floxed Inverted Open reading frame) architecture &mdash; also called FLEX (FLip-EXcision) or Cre-ON &mdash; is the standard approach for restricting AAV transgene expression to defined cell populations in Cre-driver transgenic mouse lines. The transgene ORF is placed in an antisense (inverted) orientation flanked by two pairs of heterotypic loxP recombination sites until Cre recombinase is present.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DIO CMV Expression Vectors use the CMV promoter for Cre-dependent and highly specific transgene expression.</li>
<li dir="ltr" role="presentation">Selection-Ready DIO Plasmids used for efficient selection of antibiotics in transduced cells, and feature SV40-Puro markers.</li>
<li dir="ltr" role="presentation">Fluorescent Reporter DIO Vectors carry copGFP or GFP for visually verifying recombination.</li>
<li dir="ltr" role="presentation">Customizable DIO Blank Backbones for insertion of specific genes of interest into a conditional framework.</li>
</ul>
<h2 dir="ltr"> How to Choose an AAV DIO/FLEX Vector</h2>
 Packaging Capacity Constraint AAV DIO vectors are limited to expression cassettes of approximately 4.2 kb due to the ~4.7 kb AAV genome capacity, including the loxP recombination architecture itself &mdash; a key consideration for larger transgenes. Cre-Driver Line Compatibility The vector must be paired with a Cre-driver transgenic mouse line expressing Cre in the cell population of interest, or with co-delivery of a Cre-expressing vector for cell-culture validation. Serotype for Target Region As with standard AAV over-expression, serotype selection determines tissue tropism &mdash; critical for targeting specific brain regions or cell types in neuroscience applications. Permanence of Activation Once Cre flips the ORF into sense orientation and excises one site from each loxP pair, the transgene is permanently locked in the ON orientation &mdash; heritable expression is maintained without continued Cre activity. Validation Strategy Before in vivo injection, co-transfection of the AAV DIO vector with a Cre expression plasmid in HEK293T cells confirms efficient inversion and expected expression; transfecting the DIO vector alone confirms the absence of leaky expression. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
AAV DIO (Double-Floxed Inverted Orientation) vectors are widely used for cell-type-specific conditional gene expression in Cre-driver animal models, supporting applications such as circuit mapping, optogenetics, chemogenetics, and in vivo functional studies. Because transgene expression depends on Cre-mediated recombination, researchers should verify Cre-driver specificity and recombination efficiency in their chosen model before large-scale experiments. Low-level Cre-independent ("leaky") expression can occasionally occur in DIO systems and should be evaluated using appropriate control vectors. The practical packaging capacity of AAV is approximately 4.7 kb, which may limit the size of the transgene cassette that can be accommodated. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get a quote for premium AAV DIO vector requirements.

Cre-Inducible Gene Expression (DIO) Vectors

Vector Brochure

AAV–General Guideline to Serotype Selection

AAV-Infection Guideline

Lentivirus-Infection Guideline

AAV-CMV-DIO-Blank-SV40-GFP Vector

AAV-CMV-DIO-Blank-SV40-GFP Control Vector (AAV104) by Applied Biological Materials is a 1.0 µg insert-empty Cre-inducible DIO AAV plasmid with CMV-DIO blank backbone and constitutive SV40-GFP reporter, for use as a GFP-monitored negative control in Cre-On AAV DIO expression experiments. Store at –20°C or below.