Name: 3L PC Wide-mouth High Efficient Erlenmeyer Flasks
SKU: 786501
Availability: InStock

Erlenmeyer Flasks

<h2 dir="ltr">What are Cell Culture Erlenmeyer Flasks?</h2>
 Cell culture Erlenmeyer flasks (also called shake flasks or conical flasks) are single-use, sterile plastic vessels with a conical body, flat base, and narrow neck used for suspension cell culture on orbital shakers and incubator shakers, microbial culture, and media preparation. Unlike rectangular T-flasks designed for static monolayer culture, Erlenmeyer flasks are engineered for shaking: their wide conical base maximizes liquid surface area for oxygen transfer, while the narrow neck minimizes evaporation and contamination risk. Choose PC Erlenmeyer flasks for autoclavable reuse and high-temperature media preparation; choose PETG for single-use suspension culture and processes requiring BPA-free materials.
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<h2 dir="ltr">What you will find:</h2>
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<a href="erlenmeyer-flasks/petg-erlenmeyer-flasks">PETG Erlenmeyer flasks: </a>strong choice for cell culture applications that need excellent clarity, reliable handling, and convenient single-use performance. They are lightweight, durable, and ideal for shaker culture, media preparation, and general suspension cell workflows.
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<a href="erlenmeyer-flasks/pc-erlenmeyer-flasks">PC Erlenmeyer flasks: </a>built for applications that require greater strength and higher impact resistance. They are well-suited for demanding lab environments, including vigorous shaking, transport, and workflows where added durability is important.
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<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/consumables/cell-culture/erlenmeyer-flasks/erlenmeyer-flask-accessories">Erlenmeyer Flask Accessories: </a>Includes bi-directional, inverted, and multifunctional transfer caps, plus silicon tube assemblies for 2 L, 3 L, 3 L wide-mouth, and 5 L high-efficiency flasks, supporting sterile transfer and closed-system workflows.</li>
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<h2 dir="ltr"> How to Choose Erlenmeyer Flasks for Cell Culture</h2>
 PC vs. PETG Material PC (polycarbonate) Erlenmeyer flasks are autoclavable at 121 &deg;C (typically 5&ndash;10 cycles), shatter-proof, crystal-clear, and rated from &minus;135 &deg;C to +135 &deg;C. PC is ideal for labs that sterilize media or wash in-house. PC contains BPA. PETG Erlenmeyer flasks are single-use, BPA-free, rated from &minus;80 &deg;C to +60 &deg;C, and cannot be autoclaved. They provide a contamination-free, cleaning-free alternative to glass and PC for high-throughput suspension culture operations. Flat Base vs. Baffled Base Flat-base Erlenmeyer flasks provide gentler swirling for slow-growth cell lines and media mixing. Baffled-base flasks have three or four angled internal baffles that interrupt the swirling flow, increasing turbulence, raising dissolved oxygen (DO) by up to 2&times; compared to flat-base flasks at the same orbital speed, and reducing cell sedimentation. Baffled flasks are the standard for CHO-S, HEK293-F, Sf9, microbial, and yeast cultures requiring high oxygen transfer rates. NEST Scientific offers baffled and flat-base versions in both PC and PETG across all core volumes. Vented vs. Plug Seal Cap Vented caps contain a 0.22 &micro;m hydrophobic PTFE membrane that allows free O₂/CO₂ gas exchange while excluding microbial contamination. The NEST vented cap features an oversized vent area that improves gas exchange beyond standard-size membrane caps, supporting oxygen-demanding suspension cultures at higher cell densities. Plug seal caps create a fully sealed environment for media storage, static incubation, or transport outside the incubator. Always use vented caps for actively growing suspension cultures on an orbital shaker. Volume and Fill Ratio Erlenmeyer flasks should be filled to no more than 20&ndash;25% of the nominal flask volume for suspension shaker culture to maintain adequate headspace for oxygen transfer. A 500 mL flask running at 20% fill holds 100 mL of culture; a 1,000 mL flask holds 200 mL. Overfilling reduces oxygen transfer efficiency and creates foam that blocks the vented cap membrane. For media storage (no shaking), the fill volume can approach 80% of nominal. Shaker Compatibility NEST Scientific Erlenmeyer flasks (125 mL&ndash;3,000 mL) are compatible with standard laboratory orbital shakers, including those from Infors HT, New Brunswick Scientific (Innova), and Thermo Scientific MaxQ. Confirm flask neck O.D. and base diameter against your shaker's flask holder clips or clamp system before ordering large quantities. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade polycarbonate, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PETG Erlenmeyer flasks are E-beam sterilized and certified DNase/RNase-free, pyrogen-free per lot. Vented cap PTFE membranes are 0.22 &micro;m with an oversized vent area that increases gas exchange efficiency vs. standard-diameter membrane caps. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Flask catalog sizes extend to high-efficiency 2 L and 5 L PC formats (NEST High Efficiency Erlenmeyer Flask line) for production-scale suspension culture. MBP stocks the NEST Scientific Erlenmeyer flask line across all major volumes and configurations. 
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Support accurate mixing and culturing with premium Erlenmeyer flasks designed for modern laboratories. <a href="https://www.mbpinc.net/contact-us" title="Contact the MBP Team" style="color: #236fa1; text-decoration: underline;">Contact the MBP team</a>&nbsp;for a formal quote.

<h2 dir="ltr">What are PC Erlenmeyer Flasks?</h2>
 PC (polycarbonate) Erlenmeyer flasks are autoclavable, shatter-proof, optically clear shake flasks manufactured from USP Class VI medical-grade polycarbonate for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. Polycarbonate provides a working temperature range of &minus;135 &deg;C to +135 &deg;C and withstands 5&ndash;10 autoclave cycles at 121 &deg;C &mdash; the defining advantage of PC over PETG for labs that steam-sterilize media or require reusable, re-sterilizable shake flasks. PC Erlenmeyer flasks are the correct choice when autoclavability, reuse, or processing at elevated temperature (&gt;60 &deg;C) is required; for single-use BPA-free workflows, PETG Erlenmeyer flasks are the alternative.
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<h2 dir="ltr">What you will find:</h2>
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Vent Filter Caps featuring 0.22&micro;m hydrophobic membranes to facilitate continuous, sterile gas exchange while preventing contamination and evaporation.
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Seal Caps designed for airtight liquid storage, transport, and non-aerated incubation steps.
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Flat Bottom Flasks optimized for uniform heating and stability on standard orbital shakers.
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Baffled Bottom Flasks engineered with integrated "flutes" to increase turbulence and oxygen mass transfer, ideal for high-density yeast and bacterial cultures.
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Volume Scalability with a comprehensive range from 125mL pilots to 5L high-efficiency production vessels, allowing for a seamless transition from bench-scale research to bulk bioprocessing.
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High-Efficiency Wide-Mouth Designs facilitate&nbsp;rapid filling and easy access for large-volume pipetting and automated dispensers.
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<h2 dir="ltr"> How to Choose PC Erlenmeyer Flasks</h2>
 Flat Base vs. Baffled Base for Your Cell Line Flat-base PC flasks produce laminar rotational flow during orbital shaking, providing low-shear mixing appropriate for shear-sensitive primary cells, some hybridoma lines, and media preparation. Baffled-base PC flasks generate turbulence during shaking that substantially increases dissolved oxygen (DO) transfer &mdash; critical for fast-growing CHO-S, HEK293-F, Expi293, bacterial (E. coli, B. subtilis), and yeast cultures where DO depletion limits cell density and productivity. NEST Scientific offers both base geometries across all standard volumes from 125 mL to 3,000 mL, with High Efficiency baffled formats extending to 5,000 mL for production-scale operations. Vented Cap vs. Plug Seal Cap Vented caps (0.22 &micro;m hydrophobic PTFE membrane, HDPE housing) allow continuous gas exchange during shaker culture while maintaining a sterile barrier. The NEST oversized vented cap membrane provides greater gas transfer area than standard-dimension vented caps, supporting high-density cultures. Plug seal caps are gas-impermeable HDPE caps used for static media storage in PC flasks, transport between incubators, and autoclave cycles (caps must be loosened 1&ndash;1.5 turns before autoclaving to prevent pressure buildup). Never use a sealed plug cap on a flask in an orbital shaker incubator &mdash; medium acidification and DO depletion occur within hours. Volume Selection and Fill Ratio NEST Scientific PC flasks are available in 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, 3,000 mL, and 5,000 mL (High Efficiency). Working fill for shaker culture: 20&ndash;25% of nominal volume. Working volumes: 25&ndash;30 mL in 125 mL; 50&ndash;60 mL in 250 mL; 100&ndash;125 mL in 500 mL; 200&ndash;250 mL in 1,000 mL; 400&ndash;500 mL in 2,000 mL. Scale-up from T-flask to Erlenmeyer: transition from T75 (15&ndash;25 mL static) to a 125 mL PC baffled flask when moving to suspension-adapted cell lines requiring orbital shaking. Autoclave Protocol for PC Flasks PC Erlenmeyer flasks can be autoclaved at 121 &deg;C for 15&ndash;20 minutes (standard gravity cycle). Loosen cap 1&ndash;1.5 turns before autoclaving; retighten after cooling. Repeated autoclaving causes progressive hydrolytic degradation of carbonate linkages &mdash; inspect for crazing, discoloration, or dimensional distortion after each cycle and retire flask at first sign of damage. Do not use autoclaved PC flasks for vacuum applications. BPA Consideration Polycarbonate contains bisphenol A (BPA) as an inherent part of its monomer chemistry. For applications involving estrogen receptor&ndash;positive cell lines (MCF-7, T47D, ZR-75), primary endocrine cells, or long-term culture runs exceeding 14 days, PETG Erlenmeyer flasks (BPA-free) are the safer choice. For standard bacterial, yeast, CHO, and HEK293 suspension culture, trace BPA from USP Class VI PC does not affect results in published culture protocols. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade PC resin in ISO 8 cleanrooms, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PC tensile strength: 55&ndash;75 MPa; maintains impact resistance &gt;50 J/m at &minus;40 &deg;C. Vented cap 0.22 &micro;m hydrophobic PTFE membrane confirmed non-pyrogenic. Flask base diameter and neck geometry are designed for compatibility with standard orbital shaker clips from Infors HT, New Brunswick Innova, and Thermo Scientific MaxQ platforms &mdash; confirm flask O.D. against your specific shaker clamp before bulk ordering. NEST Scientific PC Erlenmeyer flasks up to 5 L (High Efficiency line) are stocked by MBP.
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Connect with the <a href="https://mbpinc.net/contact-us" title="MBP team" style="color: #236fa1; text-decoration: underline;">MBP team</a> today to secure the exact volumes and cap configurations required for your laboratory&rsquo;s next breakthrough.

PC Erlenmeyer Flasks

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3L PC Wide-mouth High Efficient Erlenmeyer Flasks

NEST Scientific 3L wide-mouth PC Erlenmeyer flasks, E-beam SAL 10⁻⁶, USP Class VI, autoclavable. Four variants: baffled seal cap (786505), baffled vent cap (786515), flat-base seal cap (786501), flat-base vent cap (786511). DNase/RNase-free. 1/pk, 4/cs.