Name: Passive Conjugation Kit
Brand: Molecular Depot
SKU: B2015391
Price: 1395.00 USD
Availability: InStock

<h2>What are bioconjugation kits?</h2>
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Bioconjugation kits provide the chemistry needed to covalently attach a label, such as an enzyme (HRP or AP), biotin, or a fluorophore, directly to your own antibody or other protein, rather than purchasing a pre-conjugated reagent from a catalog or relying on an indirect secondary antibody detection step. Most modern kits target free primary amine groups concentrated on the antibody's lysine residues, and the entire labeling reaction can occur within a single tube without a separate purification or clean-up step.
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<h2 dir="ltr">What you will find: </h2>
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Active Labeling Matrix Modifiers: Pre-measured enzyme or fluorophore complexes prepared for efficient, stable attachment to target amino groups or sulfhydryl sites.
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Gentle Reaction Buffers: Calibrated liquid environments engineered to accelerate cross-linking while keeping sensitive primary antibodies completely stable.
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High-Recovery Separation Columns: Rapid desalting or filtration steps designed to isolate your newly labeled conjugates and remove unreacted tags quickly.
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<h2>How to choose a bioconjugation kit</h2>
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Choose a label matched to your downstream detection method
HRP or AP enzyme conjugates suit colorimetric or chemiluminescent ELISA and western blot detection, biotin conjugates pair with a streptavidin-based detection step for signal amplification, and fluorophore conjugates suit flow cytometry, immunofluorescence, and other fluorescence-based imaging applications.
Confirm your antibody is in a clean, additive-free buffer
Common antibody stabilizers like BSA, glycine, and sodium azide contain free amine groups that compete with your antibody for the labeling reagent during conjugation, so removing or avoiding these additives before the reaction improves labeling efficiency and conjugate quality.
Decide between a standard and a rapid conjugation chemistry
Standard conjugation chemistries can take up to a few hours to complete, while rapid or "fast" kit variants from several manufacturers complete the same reaction in as little as 15 minutes, trading some flexibility for speed.
Confirm whether a purification step is needed after labeling
Several modern bioconjugation kits are designed so the entire reaction occurs in one tube without separating unbound label afterward, while other chemistries, particularly fluorophore labeling with reactive dyes, may still require removing free dye via a purification column to reduce background in downstream applications.
Plan your antibody quantity around the kit's reaction scale
Bioconjugation kits are typically designed for a specific antibody quantity per reaction, often in the microgram range, so confirming your available antibody amount matches the kit's intended scale avoids running out of material mid-protocol.
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<h2>Specifications context</h2>
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Antibody bioconjugation reactions that target the lysine residue's free amine groups typically achieve efficient labeling at room temperature within 15 minutes for rapid kit chemistries or up to 3 hours for standard chemistries, with several modern kit formats completing the entire reaction in one tube and no separation step required. As of 2026, single-step, no-purification bioconjugation chemistry continues to displace older multi-step antibody labeling protocols specifically because it reduces both hands-on time and antibody loss during the conjugation process.
<a href="https://mbpinc.net/contact-us">Message the helpful MBP team</a> directly to discover the ideal bioconjugation configuration for your specific molecular targets.

Bioconjugation Kits

Molecular Depot

Key Specifications

Passive Conjugation Kit

Passive Conjugation Kit by Molecular Depot is a passive conjugation kit for coupling proteins to bare citrate-stabilised gold particles of 40 and 80 nm at optical density 20. It is used to conjugate antibodies and proteins to gold nanoparticles, in lateral flow and immunochromatographic test development and in gold nanoparticle bioconjugation. Each unit contains 1 Kit. MBP supplies Molecular Depot immunoassay reagents to research labs in the United States, Canada and worldwide.