Name: Nickel NTA Agarose Beads
Brand: Molecular Depot
SKU: B2015058
Price: 695.00 USD
Availability: InStock

<h2 data-section-id="ibnw6z" data-start="191" data-end="252">What are agarose beads and resins for immunoprecipitation?</h2>
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Agarose beads and resins are porous particles, often cross-linked agarose or Sepharose, coated with Protein A, Protein G, or a combination of both, used as the solid-phase support that captures an antibody-antigen complex during immunoprecipitation. Their porous structure gives them a high surface area and antibody-binding capacity, which is why agarose remains the higher-capacity option for preparative-scale immunoprecipitation even as magnetic beads have gained popularity for routine, smaller-scale work.
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<h2 dir="ltr">What you will find: </h2>
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Cross-Linked Spherical Beads: Highly uniform, spherical matrix structures engineered to maximize total surface exposure and improve target contact during incubation steps.
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High-Capacity Capture Matrices: Density-optimized bead structures prepared to hold heavy target loads securely without showing physical deformation under standard centrifugal forces.
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Pre-Activated Chemical Links: Coated bead selections prepared with stable functional groups to capture target biomolecules out of crude sample mixtures rapidly.
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<h2 data-section-id="19pjrjp" data-start="927" data-end="968">How to choose agarose beads and resins</h2>
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Choose Protein A or Protein G coating based on antibody species and class
Protein A generally costs less and binds rabbit antibodies well, while Protein G binds a broader range of antibody classes and species, including most mouse antibodies, so the choice should match your specific antibody's source and class.
Confirm binding capacity needs for your scale of preparation
Agarose's porous structure gives it higher antibody-binding capacity than magnetic beads, which matters when scaling up an immunoprecipitation for downstream applications needing larger quantities of purified protein.
Decide whether centrifugation steps fit your workflow
Agarose requires low-speed centrifugation to pellet the beads between wash steps, which is standard practice but can introduce some bead loss and variability compared with magnetic separation, a tradeoff worth weighing against the higher binding capacity agarose offers.
Consider a combined Protein A/G agarose resin for mixed antibody panels
A combined Protein A/G resin binds a broader range of antibody species and classes than either protein alone, which simplifies sourcing when a lab runs immunoprecipitations with antibodies from multiple species.
Confirm storage and handling requirements
Agarose bead slurries are typically supplied in a buffer with a preservative such as sodium azide and should be stored at 4&deg;C without freezing, since freezing can disrupt the bead structure and reduce binding capacity.
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<h2 data-section-id="1fljvas" data-start="3089" data-end="3114">Specifications context</h2>
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Agarose beads are most frequently used in protein purification because of their high porosity, which provides a large surface area for antibody binding even though that same porosity can become a limitation when the antibody quantity available cannot meet the resin's binding capacity. Agarose remains the preferred support over magnetic beads specifically for preparative-scale work where total protein yield matters more than the convenience of magnetic separation.
<a href="https://mbpinc.net/contact-us">Request a targeted product quote</a> from the MBP sales desk today to support your long-term protein purification schedules.

Agarose Beads

Molecular Depot

Key Specifications

Nickel NTA Agarose Beads

Nickel NTA Agarose Beads by Molecular Depot is a nickel nitrilotriacetic acid (NTA) agarose resin with a binding capacity of about 50 mg per mL. Nitrilotriacetic acid holds a nickel ion through four coordination positions and leaves two free, and a run of histidines on a tagged protein occupies those two, which is how the tag is captured. It is used to purify histidine-tagged recombinant proteins, to immobilise tagged proteins for pull-down assays and in affinity chromatography. Each unit contains 5 mL. MBP supplies Molecular Depot immunoassay reagents to research labs in the United States, Canada and worldwide.